دورية أكاديمية
GC/MS analysis, anti-leishmanial and relaxant activity of essential oil of Chenopodium ambrosioides (L.) from Malakand region.
| العنوان: | GC/MS analysis, anti-leishmanial and relaxant activity of essential oil of Chenopodium ambrosioides (L.) from Malakand region. |
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| المؤلفون: | Ali N; Department of Pharmacology, Institute of Basic Medical Sciences, Khyber Medical, University, Peshawar, KPK, Pakistan., Nabi M; Department of Pharmacology, Institute of Basic Medical Sciences, Khyber Medical, University, Peshawar, KPK, Pakistan., Shoaib M; Department of Pharmacy, University of Malakand Chakdara, Dir Lower., Shah I; Department of Pharmacy, University of Malakand Chakdara, Dir Lower., Ahmed G; Drug Regulatory Authority of Pakistan, Islamabad., Shakirullah -; Department of Pharmacology, Institute of Basic Medical Sciences, Khyber Medical, University, Peshawar, KPK, Pakistan., Ziauddin -; Department of Pharmacology, Institute of Basic Medical Sciences, Khyber Medical, University, Peshawar, KPK, Pakistan., Shah SWA; Department of Pharmacy, University of Malakand Chakdara, Dir Lower., Ghias M; Department of Pharmacy, University of Malakand Chakdara, Dir Lower., Khan S; Department of Pharmacy, University of Malakand Chakdara, Dir Lower., Ali W; Department of Pharmacy, Abasyn University, Peshawar, Khyber Pakhtunkhwa, Pakistan. |
| المصدر: | Pakistan journal of pharmaceutical sciences [Pak J Pharm Sci] 2021 Mar; Vol. 34 (2), pp. 577-583. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Faculty of Pharmacy, University of Karachi Country of Publication: Pakistan NLM ID: 9426356 Publication Model: Print Cited Medium: Internet ISSN: 1011-601X (Print) Linking ISSN: 1011601X NLM ISO Abbreviation: Pak J Pharm Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Karachi : Faculty of Pharmacy, University of Karachi, [1988- |
| مواضيع طبية MeSH: | Chenopodium ambrosioides*, Antiprotozoal Agents/*pharmacology , Jejunum/*drug effects , Leishmania tropica/*drug effects , Muscle, Smooth/*drug effects , Oils, Volatile/*pharmacology , Parasympatholytics/*pharmacology, Animals ; Gas Chromatography-Mass Spectrometry ; Oils, Volatile/chemistry ; Plant Oils/chemistry ; Plant Oils/pharmacology ; Rabbits |
| مستخلص: | Chenopodium ambrosioides is abundantly available in Malakand region. As constituents and concentrations of essential oils vary based on its geographical location, we carried our current study to extract and evaluate its possible relaxant activity in rabbits' jejunum and anti-leishmanial activity against promastigotes of Leishmania tropica. The essential oil was obtained from aerial fresh parts through steam distillation followed by GC/MS analysis. Antispasmodic activity was performed on spontaneous and KCl induced contractions. Curves for calcium concentration response (CCRCs) were prepared with and without different concentrations of essential oils and verapamil - a standard calcium channel blocker as per our reported procedures. GC/MS analysis indicated that the essential oil contains 4-carene (56.59%) and o-cymene (41.46%), the two most abundant compounds previously reported from this species. The LD50 value for acute toxicity is 279.66±2.2mg/kg. The essential oil have significant antileishmanial activity with LC50 of Log10 (1.83±0.0026) ×10-6mg/ml, potent relaxant activity on rabbits' jejunal preparations with respective EC50 = 1.46±0.15mg/ml for spontaneous activity. For KCl (80mM) induced contractions, EC50=0.26±0.02mg/ml. In CCRCs, the oil produced a right shift as exhibited by verapamil. More, its relaxant activity, which is mediated through calcium channel blocking mechanism, proves a rationale for its traditional use in gut spasm. |
| المشرفين على المادة: | 0 (Antiprotozoal Agents) 0 (Oils, Volatile) 0 (Parasympatholytics) 0 (Plant Oils) |
| تواريخ الأحداث: | Date Created: 20210719 Date Completed: 20220120 Latest Revision: 20220120 |
| رمز التحديث: | 20240628 |
| PMID: | 34275832 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1011-601X |
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