دورية أكاديمية
Using Whole Genome Sequences to Investigate Adenovirus Outbreaks in a Hematopoietic Stem Cell Transplant Unit.
| العنوان: | Using Whole Genome Sequences to Investigate Adenovirus Outbreaks in a Hematopoietic Stem Cell Transplant Unit. |
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| المؤلفون: | Myers CE; Cambridge Clinical Microbiology and Public Health Laboratory, Public Health England, Cambridge, United Kingdom., Houldcroft CJ; Department of Medicine, University of Cambridge, Cambridge, United Kingdom., Roy S; Division of Infection and Immunity, University College London, London, United Kingdom., Margetts BK; Division of Infection, Immunity and Inflammation, Great Ormond Street Institute of Child Health, University College London, London, United Kingdom., Best T; Department of Microbiology, Virology and Infection Prevention and Control, Great Ormond Street Hospital for Children National Health Service Foundation Trust, London, United Kingdom., Venturini C; Division of Infection and Immunity, University College London, London, United Kingdom., Guerra-Assunção JA; Division of Infection and Immunity, University College London, London, United Kingdom., Williams CA; Division of Infection and Immunity, University College London, London, United Kingdom., Williams R; Division of Infection and Immunity, University College London, London, United Kingdom., Dunn H; Department of Microbiology, Virology and Infection Prevention and Control, Great Ormond Street Hospital for Children National Health Service Foundation Trust, London, United Kingdom., Hartley JC; Department of Microbiology, Virology and Infection Prevention and Control, Great Ormond Street Hospital for Children National Health Service Foundation Trust, London, United Kingdom., Rao K; Department of Microbiology, Virology and Infection Prevention and Control, Great Ormond Street Hospital for Children National Health Service Foundation Trust, London, United Kingdom., Rolfe KJ; Cambridge Clinical Microbiology and Public Health Laboratory, Public Health England, Cambridge, United Kingdom., Breuer J; Division of Infection and Immunity, University College London, London, United Kingdom.; Department of Microbiology, Virology and Infection Prevention and Control, Great Ormond Street Hospital for Children National Health Service Foundation Trust, London, United Kingdom. |
| المصدر: | Frontiers in microbiology [Front Microbiol] 2021 Jul 02; Vol. 12, pp. 667790. Date of Electronic Publication: 2021 Jul 02 (Print Publication: 2021). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
| مستخلص: | A recent surge in human mastadenovirus (HAdV) cases, including five deaths, amongst a haematopoietic stem cell transplant population led us to use whole genome sequencing (WGS) to investigate. We compared sequences from 37 patients collected over a 20-month period with sequences from GenBank and our own database of HAdVs. Maximum likelihood trees and pairwise differences were used to evaluate genotypic relationships, paired with the epidemiological data from routine infection prevention and control (IPC) records and hospital activity data. During this time period, two formal outbreaks had been declared by IPC, while WGS detected nine monophyletic clusters, seven were corroborated by epidemiological evidence and by comparison of single-nucleotide polymorphisms. One of the formal outbreaks was confirmed, and the other was not. Of the five HAdV-associated deaths, three were unlinked and the remaining two considered the source of transmission. Mixed infection was frequent (10%), providing a sentinel source of recombination and superinfection. Immunosuppressed patients harboring a high rate of HAdV positivity require comprehensive surveillance. As a consequence of these findings, HAdV WGS is being incorporated routinely into clinical practice to influence IPC policy contemporaneously. Competing Interests: JB has declared that the adenovirus bait designs are used with permission from Agilent. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Myers, Houldcroft, Roy, Margetts, Best, Venturini, Guerra-Assunção, Williams, Williams, Dunn, Hartley, Rao, Rolfe and Breuer.) |
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| فهرسة مساهمة: | Keywords: adenovirus; epidemiology; nosocomial transmission; pediatric infectious disease; whole genome sequencing |
| تواريخ الأحداث: | Date Created: 20210719 Latest Revision: 20210720 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC8284422 |
| DOI: | 10.3389/fmicb.2021.667790 |
| PMID: | 34276599 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-302X |
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| DOI: | 10.3389/fmicb.2021.667790 |