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Exploring the Significance of the Exon 4-Skipping Isoform of the ZNF217 Oncogene in Breast Cancer.

التفاصيل البيبلوغرافية
العنوان: Exploring the Significance of the Exon 4-Skipping Isoform of the ZNF217 Oncogene in Breast Cancer.
المؤلفون: Bellanger A; Université Lyon 1, Lyon, France.; CRCL-Centre de Recherche en Cancérologie de Lyon-Inserm U1052-CNRS U5286, Lyon, France., Le DT; Université Lyon 1, Lyon, France.; INSERM, UMR1033 LYOS, Lyon, France., Vendrell J; Département de Pathologie et Oncobiologie, Laboratoire de Biologie des Tumeurs Solides, CHU Montpellier, Univ. Montpellier, Montpellier, France., Wierinckx A; Université Lyon 1, Lyon, France.; CRCL-Centre de Recherche en Cancérologie de Lyon-Inserm U1052-CNRS U5286, Lyon, France.; ProfileXpert, SFR-Est, CNRS UMR-S3453, INSERM US7, Lyon, France., Pongor LS; Department of Bioinformatics, Semmelweis University, Budapest, Hungary.; TTK 'TermészetTudományi Kutatóközpont' Momentum Cancer Biomarker Research Group, Institute of Enzymology, Budapest, Hungary., Solassol J; Département de Pathologie et Oncobiologie, Laboratoire de Biologie des Tumeurs Solides, CHU Montpellier, Univ. Montpellier, Montpellier, France.; Institut de Recherche en Cancérologie de Montpellier (IRCM), INSERM U1194, Univ. Montpellier, Montpellier, France., Lachuer J; Université Lyon 1, Lyon, France.; CRCL-Centre de Recherche en Cancérologie de Lyon-Inserm U1052-CNRS U5286, Lyon, France.; ProfileXpert, SFR-Est, CNRS UMR-S3453, INSERM US7, Lyon, France., Clezardin P; Université Lyon 1, Lyon, France.; INSERM, UMR1033 LYOS, Lyon, France., Győrffy B; Department of Bioinformatics, Semmelweis University, Budapest, Hungary.; TTK 'TermészetTudományi Kutatóközpont' Momentum Cancer Biomarker Research Group, Institute of Enzymology, Budapest, Hungary., Cohen PA; Université Lyon 1, Lyon, France.; CRCL-Centre de Recherche en Cancérologie de Lyon-Inserm U1052-CNRS U5286, Lyon, France.; INSERM, UMR1033 LYOS, Lyon, France.; ProfileXpert, SFR-Est, CNRS UMR-S3453, INSERM US7, Lyon, France.
المصدر: Frontiers in oncology [Front Oncol] 2021 Jul 02; Vol. 11, pp. 647269. Date of Electronic Publication: 2021 Jul 02 (Print Publication: 2021).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101568867 Publication Model: eCollection Cited Medium: Print ISSN: 2234-943X (Print) Linking ISSN: 2234943X NLM ISO Abbreviation: Front Oncol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Lausanne : Frontiers Research Foundation]
مستخلص: Oncogene alternative splicing events can create distinct functional transcripts that offer new candidate prognostic biomarkers for breast cancer. ZNF217 is a well-established oncogene but its exon 4-skipping isoform (ZNF217-ΔE4) has never been investigated in terms of clinical or biological relevance. Using in silico RNA-seq and RT-qPCR analyses, we demonstrated for the first time the existence of ZNF217-ΔE4 transcripts in primary breast tumors, and a positive correlation between ZNF217-ΔE4 mRNA levels and those of the wild-type oncogene ( ZNF217-WT ). A pilot retrospective analysis revealed that, in the Luminal subclass, the combination of the two ZNF217 variants (the ZNF217-ΔE4-WT gene-expression signature) provided more information than the mRNA expression levels of each isoform alone. Ectopic overexpression of ZNF217-ΔE4 in breast cancer cells promoted an aggressive phenotype and an increase in ZNF217-WT expression levels that was inversely correlated with DNA methylation of the ZNF217 gene. This study provides new insights into the possible role of the ZNF217-ΔE4 splice variant in breast cancer and suggests a close interplay between the ZNF217-WT and ZNF217-ΔE4 isoforms. Our data suggest that a dual signature combining the expression levels of these two isoforms may serve as a novel prognostic biomarker allowing better stratification of breast cancers with good prognosis and aiding clinicians in therapeutic decisions.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Bellanger, Le, Vendrell, Wierinckx, Pongor, Solassol, Lachuer, Clezardin, Győrffy and Cohen.)
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فهرسة مساهمة: Keywords: ZNF217; breast cancer; isoform; prognosis; splice variant
تواريخ الأحداث: Date Created: 20210719 Latest Revision: 20210720
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8283766
DOI: 10.3389/fonc.2021.647269
PMID: 34277402
قاعدة البيانات: MEDLINE
الوصف
تدمد:2234-943X
DOI:10.3389/fonc.2021.647269