دورية أكاديمية
Proteus mirabilis from community-acquired urinary tract infections (UTI-CA) shares genetic similarity and virulence factors with isolates from chicken, beef and pork meat.
| العنوان: | Proteus mirabilis from community-acquired urinary tract infections (UTI-CA) shares genetic similarity and virulence factors with isolates from chicken, beef and pork meat. |
|---|---|
| المؤلفون: | Sanches MS; Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil., Rodrigues da Silva C; Microorganism Research Center, Department of Dermatological, Infectious and Parasitic Diseases, Health Sciences Center, Medical School of São José Do Rio Preto, São José Do Rio Preto, São Paulo, Brazil., Silva LC; Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil., Montini VH; Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil., Lopes Barboza MG; Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil., Migliorini Guidone GH; Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil., Dias de Oliva BH; Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil., Nishio EK; Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil., Faccin Galhardi LC; Virology Laboratory, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil., Vespero EC; Department of Pathology, Clinical and Toxicological Analysis, Health Sciences Center, University Hospital of Londrina, State University of Londrina, Paraná, Brazil., Lelles Nogueira MC; Microorganism Research Center, Department of Dermatological, Infectious and Parasitic Diseases, Health Sciences Center, Medical School of São José Do Rio Preto, São José Do Rio Preto, São Paulo, Brazil., Dejato Rocha SP; Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil. Electronic address: rochaspd@uel.br. |
| المصدر: | Microbial pathogenesis [Microb Pathog] 2021 Sep; Vol. 158, pp. 105098. Date of Electronic Publication: 2021 Jul 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; Orlando : Academic Press, c1986- |
| مواضيع طبية MeSH: | Pork Meat* , Red Meat* , Urinary Tract Infections*, Animals ; Cattle ; Chickens ; Chlorocebus aethiops ; Humans ; Meat ; Proteus mirabilis/genetics ; Swine ; Vero Cells ; Virulence Factors/genetics |
| مستخلص: | Proteus mirabilis is an opportunistic pathogen associated with a variety of infections in humans, especially those in the urinary tract. The isolation of this pathogen in foods of animal origin such as meat is poorly documented and should not be neglected, in view of the zoonotic risk that this can pose to human health. Thus, the objective of this study was to evaluate the prevalence, virulence profile, and similarity between P. mirabilis strains isolated from chicken, beef, and pork meat and those causing community-acquired urinary tract infections (UTI-CA), in order to better understand the role of this bacterium as a zoonotic pathogen. P. mirabilis was isolated from the three types of meat and was found to be more prevalent in chicken. All isolates exhibited several genotypic and phenotypic virulence characteristics, such as adhesion capacity in HEp-2 cell culture, biofilm formation, cytotoxicity in Vero cells and genes that express fimbriae (mrpA, pmfA, ucaA, atfA), hemolysin (hpmA), proteases (zapA and ptA) and siderophore receptor (ireA). UTI-CA strains showed a higher prevalence of ucaA and ireA genes, whereas those from the chicken meat had a higher prevalence of the atfA gene compared with the isolates from the beef and pork meat. It was observed that chicken meat and UTI-CA strains mainly formed very strong biofilms, whereas strains isolated from beef and pork formed more weak and moderate biofilms. Several strains from meat showed close genetic similarity to those from UTI-CA and had the same virulence profiles. Thus, meats may be an important source of the dissemination of P. mirabilis responsible for causing UTIs in the community. (Copyright © 2021 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aggregative adhesion; Biofilm; Cytotoxicity; Virulence genes; Zoonotic risk |
| المشرفين على المادة: | 0 (Virulence Factors) |
| تواريخ الأحداث: | Date Created: 20210719 Date Completed: 20210818 Latest Revision: 20210818 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.micpath.2021.105098 |
| PMID: | 34280499 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-1208 |
|---|---|
| DOI: | 10.1016/j.micpath.2021.105098 |