دورية أكاديمية
أعرض في EDS

Ribosome heterogeneity in Drosophila melanogaster gonads through paralog-switching.

التفاصيل البيبلوغرافية
العنوان: Ribosome heterogeneity in Drosophila melanogaster gonads through paralog-switching.
المؤلفون: Hopes T; School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.; LeedsOmics, University of Leeds, Leeds, UK., Norris K; School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.; LeedsOmics, University of Leeds, Leeds, UK.; Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK., Agapiou M; School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.; LeedsOmics, University of Leeds, Leeds, UK.; Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK., McCarthy CGP; School of Life Sciences, Faculty of Medicine and Health Sciences, The University of Nottingham, Nottingham NG7 2RD, UK., Lewis PA; School of Cellular and Molecular Medicine, Faculty of Life Sciences, University of Bristol, Bristol BS8 1TD, UK., O'Connell MJ; School of Life Sciences, Faculty of Medicine and Health Sciences, The University of Nottingham, Nottingham NG7 2RD, UK., Fontana J; School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.; Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK., Aspden JL; School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.; LeedsOmics, University of Leeds, Leeds, UK.
المصدر: Nucleic acids research [Nucleic Acids Res] 2022 Feb 28; Vol. 50 (4), pp. 2240-2257.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
مواضيع طبية MeSH: Drosophila melanogaster*/genetics , Drosophila melanogaster*/metabolism , Ribosomes*/genetics , Ribosomes*/metabolism, Animals ; Female ; Male ; Ovary/metabolism ; Protein Biosynthesis ; Ribosomal Proteins/metabolism ; Testis/metabolism
مستخلص: Ribosomes have long been thought of as homogeneous macromolecular machines, but recent evidence suggests they are heterogeneous and could be specialised to regulate translation. Here, we have characterised ribosomal protein heterogeneity across 4 tissues of Drosophila melanogaster. We find that testes and ovaries contain the most heterogeneous ribosome populations, which occurs through a combination of paralog-enrichment and paralog-switching. We have solved structures of ribosomes purified from in vivo tissues by cryo-EM, revealing differences in precise ribosomal arrangement for testis and ovary 80S ribosomes. Differences in the amino acid composition of paralog pairs and their localisation on the ribosome exterior indicate paralog-switching could alter the ribosome surface, enabling different proteins to regulate translation. One testis-specific paralog-switching pair is also found in humans, suggesting this is a conserved site of ribosome heterogeneity. Overall, this work allows us to propose that mRNA translation might be regulated in the gonads through ribosome heterogeneity, providing a potential means of ribosome specialisation.
(© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.)
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معلومات مُعتمدة: 105615/Z/14/Z United Kingdom WT_ Wellcome Trust; MR/N000471/1 United Kingdom MRC_ Medical Research Council; BB/S007407/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; United Kingdom WT_ Wellcome Trust; BB/M011151/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
المشرفين على المادة: 0 (Ribosomal Proteins)
تواريخ الأحداث: Date Created: 20210720 Date Completed: 20220415 Latest Revision: 20220531
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8887423
DOI: 10.1093/nar/gkab606
PMID: 34283226
قاعدة البيانات: MEDLINE
الوصف
تدمد:1362-4962
DOI:10.1093/nar/gkab606