دورية أكاديمية
أعرض في EDS

Mouse experiments demonstrate differential pathogenicity and virulence of Trypanosoma brucei rhodesiense strains.

التفاصيل البيبلوغرافية
العنوان: Mouse experiments demonstrate differential pathogenicity and virulence of Trypanosoma brucei rhodesiense strains.
المؤلفون: Kipkorir LW; Department of Biological Sciences, Egerton University, P. O Box, 536-20115, Egerton, Kenya., John TK; Biotechnology Research Institute - Kenya Agricultural and Livestock Research Organisation, Chemotherapy Division, Primate Section, P.O Box, 362-00902, Kikuyu, Kenya; Department of Animal Sciences, Meru University of Science and Technology, P.O Box, 972-60200, Meru, Kenya., Owino OB; KEMRI-Wellcome Trust Research Programme, CGMRC, P. O Box, 230-80108, Kilifi, Kenya; Department of Public Health and Primary Care, Leuven Biostatistics and Statistical Bioinformatics Centre, Kapucijnenvoer 35, Blok D, Bus 7001, B-3000, Leuven, Belgium., John O; Biotechnology Research Institute - Kenya Agricultural and Livestock Research Organisation, Chemotherapy Division, Primate Section, P.O Box, 362-00902, Kikuyu, Kenya., Robert S; Department of Veterinary and Clinical Studies, Egerton University, P. O Box, 536-20115, Egerton, Kenya., Daniel M; International Centre of Insect Physiology and Ecology, P. O Box, 30772-000100, Nairobi, Kenya., Owino AV; Department of Biochemistry and Molecular Biology, Egerton University, P. O Box, 536-20115, Egerton, Kenya; International Centre of Insect Physiology and Ecology, P. O Box, 30772-000100, Nairobi, Kenya. Electronic address: vincent.adunga@egerton.ac.ke.
المصدر: Experimental parasitology [Exp Parasitol] 2021 Sep; Vol. 228, pp. 108135. Date of Electronic Publication: 2021 Jul 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 0370713 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2449 (Electronic) Linking ISSN: 00144894 NLM ISO Abbreviation: Exp Parasitol Subsets: MEDLINE
أسماء مطبوعة: Publication: Orlando, FL : Academic Press
Original Publication: New York.
مواضيع طبية MeSH: Trypanosoma brucei rhodesiense/*pathogenicity , Trypanosomiasis, African/*parasitology , Tsetse Flies/*parasitology, Animals ; Eating ; Female ; Linear Models ; Liver/pathology ; Male ; Mice ; Organ Size ; Parasitemia/parasitology ; Spleen/pathology ; Trypanosoma brucei rhodesiense/isolation & purification ; Virulence
مستخلص: Trypanosoma brucei rhodesiense is the causative agent for Rhodesian human African trypanosomiasis. The disease is considered acute, but varying clinical outcomes including chronic infections have been observed. The basis for these different clinical manifestations is thought to be associated with a combination of parasite and host factors. In the current study, Trypanosoma brucei rhodesiense strains responsible for varying infection outcomes were sought using mouse model. Clinical rHAT parasite isolates were subjected to PCR tests to confirm presence of the serum resistance associated (SRA) gene. Thereafter, four T. b. rhodesiense isolates were subjected to a comparative pathogenicity study using female Swiss white mice; the parasite strains were compared on the basis of parasitaemia, host survival time, clinical and postmortem biomarkers of infection severity. Isolates identified to cause acute and chronic disease were compared for establishment in insect vector, tsetse fly. The mouse survival time was significantly different (Log-rankp = 0.0001). With mice infected with strain KETRI 3801 exhibiting the shortest survival time (20 days) as compared to those infected with KETRI 3928 that, as controls, survived past the 60 days study period. In addition, development of anaemia was rapid in KETRI 3801 and least in KETRI 3928 infections, and followed the magnitude of survival time. Notably, hepatosplenomegaly was pronounced with longer survival. Mouse weight and feed intake reduced (KETRI 3801 > KETRI 2636 > EATRO 1762) except in KETRI 3928 infections which remained similar to controls. Comparatively, acute to chronic infection outcomes is in the order of KETRI 3801 > KETRI 2636 > EATRO 1762 > KETRI 3928, indicative of predominant role of strain dependent factors. Further, KETRI 3928 strain established better in tsetse as compared to KETRI 3801, suggesting that transmission of strains causing chronic infections could be common. In sum, we have identified Trypanosoma brucei rhodesiense strains that cause acute and chronic infections in mice, that will be valuable in investigating pathogen - host interactions responsible for varying disease outcomes and transmission in African trypanosomiasis.
(Copyright © 2021. Published by Elsevier Inc.)
References: Parasit Vectors. 2015 Oct 12;8:528. (PMID: 26458386)
Trends Parasitol. 2013 Apr;29(4):188-96. (PMID: 23507033)
PLoS Negl Trop Dis. 2014 May 22;8(5):e2747. (PMID: 24853062)
PLoS Negl Trop Dis. 2010 Dec 07;4(12):e906. (PMID: 21151878)
Methods Mol Biol. 2004;262:53-86. (PMID: 14769956)
Virulence. 2014;5(8):775-85. (PMID: 25483864)
Cell. 2016 Jan 14;164(1-2):246-257. (PMID: 26771494)
Allergy Asthma Clin Immunol. 2016 Jan 22;12:4. (PMID: 26807135)
PLoS Pathog. 2010 Mar 05;6(3):e1000793. (PMID: 20221444)
Infect Immun. 2000 Dec;68(12):6511-8. (PMID: 11083759)
Clin Exp Immunol. 2005 Mar;139(3):405-10. (PMID: 15730385)
Kinetoplastid Biol Dis. 2005 Jul 14;4:5. (PMID: 16018802)
Exp Parasitol. 1976 Dec;40(3):427-31. (PMID: 976425)
Front Immunol. 2017 Sep 07;8:1105. (PMID: 28936213)
PLoS Pathog. 2015 Jun 12;11(6):e1004964. (PMID: 26070118)
Blood. 1997 Sep 15;90(6):2244-52. (PMID: 9310475)
Infect Immun. 1999 Jun;67(6):3128-32. (PMID: 10338530)
Exp Parasitol. 2005 May;110(1):30-8. (PMID: 15804376)
Adv Parasitol. 2003;53:1-83. (PMID: 14587696)
Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):12133-8. (PMID: 19587241)
Mol Microbiol. 2006 Jun;60(5):1194-204. (PMID: 16689795)
Lancet. 2001 Dec 15;358(9298):2017-9. (PMID: 11755607)
Infect Immun. 1995 Apr;63(4):1563-6. (PMID: 7890423)
Infect Immun. 2010 Mar;78(3):1096-108. (PMID: 20086091)
Acta Trop. 1990 Oct;48(1):9-15. (PMID: 1980807)
PLoS Pathog. 2017 Apr 10;13(4):e1006324. (PMID: 28394929)
PLoS Negl Trop Dis. 2012;6(2):e1515. (PMID: 22348165)
Trop Med Parasitol. 1987 Sep;38(3):167-70. (PMID: 3432950)
Nature. 2014 Jan 30;505(7485):681-685. (PMID: 24336212)
Parasitol Today. 1990 Jan;6(1):14-7. (PMID: 15463248)
Elife. 2014 May 20;3:e02324. (PMID: 24844706)
J Med Case Rep. 2014 Feb 14;8:53. (PMID: 24529084)
Parasite Immunol. 1994 Jan;16(1):51-4. (PMID: 7908735)
Kinetoplastid Biol Dis. 2004 Sep 17;3(1):3. (PMID: 15377385)
Trop Med Parasitol. 1988 Mar;39(1):56-8. (PMID: 3387828)
PLoS Negl Trop Dis. 2008;2(5):e238. (PMID: 18846231)
Science. 2000 Nov 10;290(5494):1166-8. (PMID: 11073456)
Infect Immun. 2002 Mar;70(3):1342-51. (PMID: 11854219)
Parasitology. 1995 Sep;111 ( Pt 3):289-300. (PMID: 7567097)
Curr Opin Microbiol. 2008 Aug;11(4):345-51. (PMID: 18621142)
J Immunol. 2005 Aug 15;175(4):2501-9. (PMID: 16081822)
Proc Biol Sci. 2001 Nov 7;268(1482):2235-43. (PMID: 11674871)
Parasitol Res. 2005 Feb;95(3):186-92. (PMID: 15619129)
PLoS Negl Trop Dis. 2010 Nov 02;4(11):e868. (PMID: 21072230)
Mol Biochem Parasitol. 1997 Dec 1;90(1):269-80. (PMID: 9497048)
Med Vet Entomol. 2006 Dec;20(4):388-92. (PMID: 17199750)
Am J Trop Med Hyg. 2002 Dec;67(6):684-90. (PMID: 12518862)
Int J Parasitol. 1998 Feb;28(2):219-40. (PMID: 9512986)
Front Cell Infect Microbiol. 2013 Sep 18;3:53. (PMID: 24066283)
PLoS Pathog. 2013;9(4):e1003318. (PMID: 23637607)
PLoS Negl Trop Dis. 2011 Mar 01;5(3):e968. (PMID: 21407802)
Lancet Neurol. 2013 Feb;12(2):186-94. (PMID: 23260189)
Tropenmed Parasitol. 1982 Dec;33(4):255-9. (PMID: 7164167)
J Cell Sci. 1997 Nov;110 ( Pt 21):2661-71. (PMID: 9427384)
معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust; 101166 United Kingdom WT_ Wellcome Trust; 203077 United Kingdom WT_ Wellcome Trust
فهرسة مساهمة: Keywords: African trypanosome; Pathogenesis; Sleeping sickness; Trypanosoma brucei rhodesiense.; Virulence
تواريخ الأحداث: Date Created: 20210720 Date Completed: 20210826 Latest Revision: 20220910
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7613321
DOI: 10.1016/j.exppara.2021.108135
PMID: 34284027
قاعدة البيانات: MEDLINE
الوصف
تدمد:1090-2449
DOI:10.1016/j.exppara.2021.108135