دورية أكاديمية
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Cytotoxicity and Apoptosis Induced by Chenopodium ambrosioides L. Essential Oil in Human Normal Liver Cell Line L02 via the Endogenous Mitochondrial Pathway Rather Than the Endoplasmic Reticulum Stress.

التفاصيل البيبلوغرافية
العنوان: Cytotoxicity and Apoptosis Induced by Chenopodium ambrosioides L. Essential Oil in Human Normal Liver Cell Line L02 via the Endogenous Mitochondrial Pathway Rather Than the Endoplasmic Reticulum Stress.
المؤلفون: Wang XY; West China School of Public Health, Sichuan University, Chengdu 610017, China.; School of Medical Technology, Suzhou Vocational Health College, Suzhou 215009, China., Hao JM; College of Life Science, Sichuan Normal University, Chengdu 610101, China., Ren QR; College of Life Science, Sichuan Normal University, Chengdu 610101, China., Li HY; College of Life Science, Sichuan Normal University, Chengdu 610101, China., Wu JS; College of Life Science, Sichuan Normal University, Chengdu 610101, China., Zhu XH; College of Life Science, Sichuan Normal University, Chengdu 610101, China., Chen JY; West China School of Public Health, Sichuan University, Chengdu 610017, China., Wang YN; College of Life Science, Sichuan Normal University, Chengdu 610101, China., Zhang LS; West China School of Public Health, Sichuan University, Chengdu 610017, China.
المصدر: International journal of environmental research and public health [Int J Environ Res Public Health] 2021 Jul 13; Vol. 18 (14). Date of Electronic Publication: 2021 Jul 13.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101238455 Publication Model: Electronic Cited Medium: Internet ISSN: 1660-4601 (Electronic) Linking ISSN: 16604601 NLM ISO Abbreviation: Int J Environ Res Public Health Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI, c2004-
مواضيع طبية MeSH: Chenopodium ambrosioides* , Oils, Volatile*/toxicity, Apoptosis ; Cell Line ; Endoplasmic Reticulum Stress ; Female ; Humans ; Liver
مستخلص: Chenopodium ambrosioides L. ( C. ambrosioides) has been used as dietary condiments and as traditional medicine in South America. The oil of Chenopodium ambrosioides L. ( C. ambrosioides) can be used as a natural antioxidant in food processing. It also has analgesic, sedating, and deworming effects, and can be used along with the whole plant for its medical effects: decongestion, as an insecticide, and to offer menstruation pain relief. This study was conducted to investigate the cytotoxicity and apoptosis effects of an essential oil from C. ambrosioides in vitro. The cytotoxicity evaluation of the essential oil from C. ambrosioides on human normal liver cell line L02 was assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. AO/EB dual fluorescent staining assay and Annexin V-FITC were used for apoptosis analysis. The changes in mitochondrial membrane potential (MMP) were analyzed with 5,5,6,6'-tetrachloro-1,1,3,3,-tetraethyl-imidacarbocyanine iodide (JC-1) dye under a fluorescence microscope. The level of apoptosis related protein expression was quantified by Western blot. The L02 cells were treated with the essential oil from C. ambrosioides at 24, 48, and 72 h, and the IC 50 values were 65.45, 58.03, and 35.47 μg/mL, respectively. The AO/EB staining showed that viable apoptotic cells, non-viable apoptotic cells, and non-viable non-apoptotic cells appeared among the L02 cells under the fluorescence microscope. Cell cycle arrest at the S phase and cell apoptosis increased through flow cytometry in the L02 cells treated with the essential oil. MMP decreased in a concentration-dependent manner, as seen through JC-1 staining under the fluorescence microscope. In the L02 cells as shown by Western blot and qPCR, the amount of the apoptosis-related proteins and the mRNA expression levels of cytochrome C, Bax, Caspase-9, and Caspase-3 increased, Bcl-2 decreased, and Caspase-12, which is expressed in the endoplasmic reticulum, showed no obvious changes in protein amount or mRNA expression level. The essential oil form C. ambrosioides had a cytotoxic effect on L02 cells. It could inhibit L02 cell proliferation, arrest the cell cycle at the S phase, and induce L02 cell apoptosis through the endogenous mitochondrial pathway.
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فهرسة مساهمة: Keywords: Chenopodium ambrosioides L.; apoptosis; cytotoxicity; endogenous mitochondrial pathway; essential oil
المشرفين على المادة: 0 (Oils, Volatile)
تواريخ الأحداث: Date Created: 20210724 Date Completed: 20210811 Latest Revision: 20210811
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8304090
DOI: 10.3390/ijerph18147469
PMID: 34299918
قاعدة البيانات: MEDLINE
الوصف
تدمد:1660-4601
DOI:10.3390/ijerph18147469