دورية أكاديمية
أعرض في EDS

Iron Oxidation by a Fused Cytochrome-Porin Common to Diverse Iron-Oxidizing Bacteria.

التفاصيل البيبلوغرافية
العنوان: Iron Oxidation by a Fused Cytochrome-Porin Common to Diverse Iron-Oxidizing Bacteria.
المؤلفون: Keffer JL; Department of Earth Sciences, University of Delawaregrid.33489.35, Newark, Delaware, USA., McAllister SM; School of Marine Science and Policy, University of Delawaregrid.33489.35, Newark, Delaware, USA., Garber AI; Department of Earth Sciences, University of Delawaregrid.33489.35, Newark, Delaware, USA., Hallahan BJ; Department of Earth Sciences, University of Delawaregrid.33489.35, Newark, Delaware, USA., Sutherland MC; Department of Biological Sciences, University of Delawaregrid.33489.35, Newark, Delaware, USA., Rozovsky S; Department of Chemistry and Biochemistry, University of Delawaregrid.33489.35, Newark, Delaware, USA., Chan CS; Department of Earth Sciences, University of Delawaregrid.33489.35, Newark, Delaware, USA.; School of Marine Science and Policy, University of Delawaregrid.33489.35, Newark, Delaware, USA.
المصدر: MBio [mBio] 2021 Aug 31; Vol. 12 (4), pp. e0107421. Date of Electronic Publication: 2021 Jul 27.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101519231 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2150-7511 (Electronic) NLM ISO Abbreviation: mBio Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, D.C. : American Society for Microbiology
مواضيع طبية MeSH: Cytochromes/*metabolism , Iron/*metabolism , Porins/*metabolism , Proteobacteria/*metabolism, Biochemical Phenomena ; Cytochromes/genetics ; Ferric Compounds/metabolism ; Ferrous Compounds/metabolism ; Oxidation-Reduction ; Phylogeny ; Proteobacteria/enzymology ; Proteobacteria/genetics
مستخلص: Iron (Fe) oxidation is one of Earth's major biogeochemical processes, key to weathering, soil formation, water quality, and corrosion. However, our understanding of microbial contribution is limited by incomplete knowledge of microbial iron oxidation mechanisms, particularly in neutrophilic iron oxidizers. The genomes of many diverse iron oxidizers encode a homolog to an outer membrane cytochrome (Cyc2) shown to oxidize iron in two acidophiles. Phylogenetic analyses show Cyc2 sequences from neutrophiles cluster together, suggesting a common function, though this function has not been verified in these organisms. Therefore, we investigated the iron oxidase function of heterologously expressed Cyc2 from a neutrophilic iron oxidizer Mariprofundus ferrooxydans PV-1. Cyc2 PV-1 is capable of oxidizing iron, and its redox potential is 208 ± 20 mV, consistent with the ability to accept electrons from Fe 2+ at neutral pH. These results support the hypothesis that Cyc2 functions as an iron oxidase in neutrophilic iron-oxidizing organisms. The results of sequence analysis and modeling reveal that the entire Cyc2 family shares a unique fused cytochrome-porin structure, with a defining consensus motif in the cytochrome region. On the basis of results from structural analyses, we predict that the monoheme cytochrome Cyc2 specifically oxidizes dissolved Fe 2+ , in contrast to multiheme iron oxidases, which may oxidize solid Fe(II). With our results, there is now functional validation for diverse representatives of Cyc2 sequences. We present a comprehensive Cyc2 phylogenetic tree and offer a roadmap for identifying cyc2/ Cyc2 homologs and interpreting their function. The occurrence of cyc2 in many genomes beyond known iron oxidizers presents the possibility that microbial iron oxidation may be a widespread metabolism. IMPORTANCE Iron is practically ubiquitous across Earth's environments, central to both life and geochemical processes, which depend heavily on the redox state of iron. Although iron oxidation, or "rusting," can occur abiotically at near-neutral pH, we find neutrophilic iron-oxidizing bacteria (FeOB) are widespread, including in aquifers, sediments, hydrothermal vents, pipes, and water treatment systems. FeOB produce highly reactive Fe(III) oxyhydroxides that bind a variety of nutrients and toxins; thus, these microbes are likely a controlling force in iron and other biogeochemical cycles. There has been mounting evidence that Cyc2 functions as an iron oxidase in neutrophiles, but definitive proof of its function has long eluded us. This work provides conclusive biochemical evidence of iron oxidation by Cyc2 from neutrophiles. Cyc2 is common to a wide variety of iron oxidizers, including acidophilic and phototrophic iron oxidizers, suggesting that this fused cytochrome-porin structure is especially well adapted for iron oxidation.
References: Nature. 1970 Aug 15;227(5259):680-5. (PMID: 5432063)
Genome Announc. 2017 Mar 30;5(13):. (PMID: 28360175)
Microbiology (Reading). 2011 Jun;157(Pt 6):1551-1564. (PMID: 21511765)
Bioinformatics. 2006 Jul 1;22(13):1658-9. (PMID: 16731699)
Environ Sci Technol. 2010 Jan 1;44(1):15-23. (PMID: 20000681)
Nat Struct Mol Biol. 2007 Jan;14(1):85-7. (PMID: 17187075)
Front Microbiol. 2012 Mar 16;3:96. (PMID: 22438853)
Adv Microb Physiol. 2016;68:87-138. (PMID: 27134022)
Appl Environ Microbiol. 2020 Nov 24;86(24):. (PMID: 33008825)
Curr Protoc Bioinformatics. 2016 Jun 20;54:5.6.1-5.6.37. (PMID: 27322406)
J Biol Chem. 2008 Sep 19;283(38):25803-11. (PMID: 18632666)
Cell. 2020 Apr 30;181(3):665-673.e10. (PMID: 32289252)
J Mol Biol. 2018 Jul 20;430(15):2237-2243. (PMID: 29258817)
Res Microbiol. 2018 Dec;169(10):618-627. (PMID: 29864487)
Nat Protoc. 2015 Jun;10(6):845-58. (PMID: 25950237)
Front Microbiol. 2020 Jan 31;11:37. (PMID: 32082281)
FEBS Lett. 2014 Mar 3;588(5):701-4. (PMID: 24440354)
J Mol Biol. 1999 Sep 17;292(2):195-202. (PMID: 10493868)
Appl Environ Microbiol. 2016 Apr 18;82(9):2656-2668. (PMID: 26896135)
FEMS Microbiol Ecol. 2019 Apr 1;95(4):. (PMID: 30715272)
Front Microbiol. 2015 Apr 28;6:332. (PMID: 25972843)
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W244-8. (PMID: 15980461)
Genome Announc. 2014 Aug 21;2(4):. (PMID: 25146142)
Biochem Soc Trans. 2012 Dec 1;40(6):1261-7. (PMID: 23176465)
Trends Microbiol. 2011 Jul;19(7):330-40. (PMID: 21664821)
Bioinformatics. 2014 May 1;30(9):1312-3. (PMID: 24451623)
Structure. 2003 May;11(5):547-55. (PMID: 12737820)
Methods Mol Biol. 2011;705:123-50. (PMID: 21125384)
J Bacteriol. 1987 Mar;169(3):929-33. (PMID: 2434461)
ISME J. 2011 Apr;5(4):717-27. (PMID: 21107443)
mBio. 2019 Nov 5;10(6):. (PMID: 31690680)
BMC Bioinformatics. 2008 Jan 23;9:40. (PMID: 18215316)
Annu Rev Microbiol. 2010;64:561-83. (PMID: 20565252)
ISME J. 2017 Aug;11(8):1900-1914. (PMID: 28362721)
mSystems. 2020 Feb 18;5(1):. (PMID: 32071158)
Biophys J. 2015 Oct 6;109(7):1429-38. (PMID: 26445443)
PeerJ. 2020 Feb 26;8:e8584. (PMID: 32149022)
Environ Microbiol. 2011 Nov;13(11):2915-29. (PMID: 21895918)
ISME J. 2008 May;2(5):542-50. (PMID: 18463612)
J Bacteriol. 2007 Mar;189(5):1765-73. (PMID: 17189359)
ISME J. 2021 May;15(5):1271-1286. (PMID: 33328652)
Environ Sci Technol. 2021 Jul 6;55(13):9362-9371. (PMID: 34110796)
J Biol Chem. 2009 Oct 16;284(42):28865-73. (PMID: 19661057)
Appl Environ Microbiol. 2015 Sep 1;81(17):5927-37. (PMID: 26092463)
ISME J. 2017 Nov;11(11):2624-2636. (PMID: 28820506)
Nucleic Acids Res. 2019 Jul 2;47(W1):W402-W407. (PMID: 31251384)
Microbiol Mol Biol Rev. 2003 Dec;67(4):593-656. (PMID: 14665678)
Nat Biotechnol. 2019 Apr;37(4):420-423. (PMID: 30778233)
J Bacteriol. 1979 Dec;140(3):902-10. (PMID: 118160)
Front Microbiol. 2013 Sep 12;4:254. (PMID: 24062729)
Front Microbiol. 2017 Jul 18;8:1280. (PMID: 28769885)
Anal Biochem. 1984 Feb;136(2):509-14. (PMID: 6202169)
Methods Mol Biol. 2012;881:63-72. (PMID: 22639210)
J Bacteriol. 2002 Jan;184(1):313-7. (PMID: 11741873)
Science. 1986 Aug 29;233(4767):948-52. (PMID: 3016897)
FEMS Microbiol Ecol. 2001 Jan;34(3):181-186. (PMID: 11137597)
Biochemistry. 2016 Jun 7;55(22):3150-6. (PMID: 27198710)
BMC Bioinformatics. 2010 Aug 18;11:431. (PMID: 20718988)
J Bacteriol. 2013 Jul;195(14):3260-8. (PMID: 23687275)
Biochem Biophys Res Commun. 1998 Oct 29;251(3):744-7. (PMID: 9790980)
ISME J. 2016 Sep;10(9):2106-17. (PMID: 26943628)
Front Microbiol. 2012 Feb 08;3:37. (PMID: 22347878)
Microbiol Resour Announc. 2020 Jan 9;9(2):. (PMID: 31919186)
Nat Commun. 2017 Oct 20;8(1):1063. (PMID: 29051484)
Protein Expr Purif. 2010 Sep;73(1):78-82. (PMID: 20303407)
Annu Rev Biochem. 2011;80:189-210. (PMID: 21370981)
FEMS Microbiol Ecol. 2020 May 1;96(5):. (PMID: 32149354)
Genome Announc. 2015 Feb 05;3(1):. (PMID: 25657271)
Trends Microbiol. 2010 Jun;18(6):266-74. (PMID: 20382024)
Hoppe Seylers Z Physiol Chem. 1971 May;352(5):725-33. (PMID: 4996284)
PLoS One. 2011;6(9):e25386. (PMID: 21966516)
PLoS One. 2014 Jul 17;9(7):e102561. (PMID: 25033299)
Electrophoresis. 2003 Oct;24(19-20):3508-16. (PMID: 14595698)
J Mol Biol. 1996 Feb 9;255(5):753-66. (PMID: 8636976)
Front Microbiol. 2015 Nov 13;6:1265. (PMID: 26617599)
Curr Protoc Bioinformatics. 2020 Dec;72(1):e108. (PMID: 33315308)
PLoS One. 2007 Aug 01;2(7):e667. (PMID: 17668050)
J Vis Exp. 2016 Jul 04;(113):. (PMID: 27404000)
BMC Bioinformatics. 2009 Dec 15;10:421. (PMID: 20003500)
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7. (PMID: 15034147)
Front Microbiol. 2017 Aug 21;8:1584. (PMID: 28871245)
Biochim Biophys Acta. 2013 Feb;1827(2):161-75. (PMID: 23044392)
معلومات مُعتمدة: P20 GM103446 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: cytochromes; environmental microbiology; iron metabolism; iron oxidizers; outer membrane proteins
المشرفين على المادة: 0 (Cytochromes)
0 (Ferric Compounds)
0 (Ferrous Compounds)
0 (Porins)
E1UOL152H7 (Iron)
SCR Organism: Mariprofundus ferrooxydans
تواريخ الأحداث: Date Created: 20210727 Date Completed: 20211223 Latest Revision: 20211223
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8406198
DOI: 10.1128/mBio.01074-21
PMID: 34311573
قاعدة البيانات: MEDLINE
الوصف
تدمد:2150-7511
DOI:10.1128/mBio.01074-21