دورية أكاديمية
Comparison of Unit Resolution Versus High-Resolution Accurate Mass for Parallel Reaction Monitoring.
| العنوان: | Comparison of Unit Resolution Versus High-Resolution Accurate Mass for Parallel Reaction Monitoring. |
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| المؤلفون: | Heil LR; Department of Genome Sciences, University of Washington, 3720 15th Street NE, Seattle, Washington 98195, United States., Remes PM; Thermo Fisher Scientific, 355 River Oaks Parkway, San Jose, California 95134, United States., MacCoss MJ; Department of Genome Sciences, University of Washington, 3720 15th Street NE, Seattle, Washington 98195, United States. |
| المصدر: | Journal of proteome research [J Proteome Res] 2021 Sep 03; Vol. 20 (9), pp. 4435-4442. Date of Electronic Publication: 2021 Jul 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101128775 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-3907 (Electronic) Linking ISSN: 15353893 NLM ISO Abbreviation: J Proteome Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c2002- |
| مواضيع طبية MeSH: | Peptides* , Proteomics*, Chromatography, Liquid ; Ions ; Mass Spectrometry |
| مستخلص: | Parallel reaction monitoring (PRM) is an increasingly popular alternative to selected reaction monitoring (SRM) for targeted proteomics. PRM's strengths over SRM are that it monitors all product ions in a single spectrum, thus eliminating the need to select interference-free product ions prior to data acquisition, and that it is most frequently performed on high-resolution instruments, such as quadrupole-orbitrap and quadrupole-time-of-flight instruments. Here, we show that the primary advantage of PRM is the ability to monitor all transitions in parallel and that high-resolution data are not necessary to obtain high-quality quantitative data. We run the same scheduled PRM assay, measuring 432 peptides from 126 plasma proteins, multiple times on an Orbitrap Eclipse Tribrid mass spectrometer, alternating separate liquid chromatography-tandem mass spectrometry runs between the high-resolution Orbitrap and the unit resolution linear ion trap for PRM. We find that both mass analyzers have similar technical precision and that the linear ion trap's superior sensitivity gives it better lower limits of quantitation for over 62% of peptides in the assay. |
| فهرسة مساهمة: | Keywords: parallel reaction monitoring; plasma; targeted proteomics |
| المشرفين على المادة: | 0 (Ions) 0 (Peptides) |
| تواريخ الأحداث: | Date Created: 20210728 Date Completed: 20211028 Latest Revision: 20211028 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1021/acs.jproteome.1c00377 |
| PMID: | 34319745 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1535-3907 |
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| DOI: | 10.1021/acs.jproteome.1c00377 |