دورية أكاديمية
Folate-methotrexate loaded bovine serum albumin nanoparticles preparation: an in vitro drug targeting cytokines overwhelming expressed immune cells from rheumatoid arthritis patients.
| العنوان: | Folate-methotrexate loaded bovine serum albumin nanoparticles preparation: an in vitro drug targeting cytokines overwhelming expressed immune cells from rheumatoid arthritis patients. |
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| المؤلفون: | Al-Rahim AM; Department of Molecular and Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq., AlChalabi R; Department of Molecular and Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq., Al-Saffar AZ; Department of Molecular and Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq., Sulaiman GM; Department of Applied Sciences, University of Technology, Baghdad, Iraq., Albukhaty S; Department of Chemistry, College of Science, University of Misan, Maysan, Iraq., Belali T; Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, University of Bisha, Bisha, Saudi Arabia., Ahmed EM; Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, University of Bisha, Bisha, Saudi Arabia.; Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, University of El Imam El Mahdi, Kosti, Sudan., Khalil KAA; Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, University of Bisha, Bisha, Saudi Arabia.; Department of Medical Laboratory Sciences, Faculty of Medicine and Health Sciences, University of Hodeidah, Hodeidah, Yemen. |
| المصدر: | Animal biotechnology [Anim Biotechnol] 2023 Apr; Vol. 34 (2), pp. 166-182. Date of Electronic Publication: 2021 Jul 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9011409 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2378 (Electronic) Linking ISSN: 10495398 NLM ISO Abbreviation: Anim Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2005- : Abingdon, Oxford : Taylor & Francis Original Publication: New York, N.Y. : Marcel Dekker, c1990- |
| مواضيع طبية MeSH: | Arthritis, Rheumatoid*/drug therapy , Nanoparticles*, Animals ; Methotrexate/therapeutic use ; Folic Acid/therapeutic use ; Serum Albumin, Bovine/therapeutic use ; Cytokines ; Drug Delivery Systems |
| مستخلص: | The study planned to estimate biological parameters linked to rheumatoid arthritis (RA) patients, detecting the influence of MTX and biotherapy treatments on these parameters and synthesizing methotrexate bovine serum albumin nanoparticles linked to folate (FA-MTX-BSA NPs) to reduce the overwhelming expression of inflammatory cytokines. Inflammatory parameters showed significant increases in newly diagnosed and MTX-receiving groups while no changes were observed in the biotherapy-maintained group. MTX-loaded BSA nanoparticles were fabricated by the desolvation method and further linked to activated folic acid to obtain FA-MTX-BSA NPs. FA-MTX-BSA NPs were successfully characterized within the nanoscale range using different screening techniques. FA-MTX-BSA NPs showed an in vitro release in a sustained manner. The potential of MTX, MTX-BSA NPs, and FA-MTX-BSA NPs in inducing cytokine level reduction was detected. Significant decreases in interleukin- 1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) levels were obtained in cultures treated with FA-MTX-BSA NPs compared to the untreated culture in a dose-dependent pattern. Furthermore, FA-MTX-BSA NPs comparing with MTX and MTX-BSA NPs exhibited a significant advanced effect in decreasing cytokines levels. Accordingly, the conjunction of BSA NPs and MTX linked to folate potentially reduced cytokines manifestation in RA. |
| فهرسة مساهمة: | Keywords: Methotrexate; bovine serum albumin; folate; nanoparticles; rheumatoid arthritis |
| المشرفين على المادة: | YL5FZ2Y5U1 (Methotrexate) 935E97BOY8 (Folic Acid) 27432CM55Q (Serum Albumin, Bovine) 0 (Cytokines) |
| تواريخ الأحداث: | Date Created: 20210728 Date Completed: 20230413 Latest Revision: 20230413 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1080/10495398.2021.1951282 |
| PMID: | 34319853 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1532-2378 |
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| DOI: | 10.1080/10495398.2021.1951282 |