دورية أكاديمية
Cold acclimation induces life stage-specific responses in the cardiac proteome of western painted turtles (Chrysemys picta bellii): implications for anoxia tolerance.
| العنوان: | Cold acclimation induces life stage-specific responses in the cardiac proteome of western painted turtles (Chrysemys picta bellii): implications for anoxia tolerance. |
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| المؤلفون: | Alderman SL; Department of Integrative Biology, University of Guelph, ON, Canada, N1G 2W1., Riggs CL; Department of Biology, Saint Louis University, St Louis, MO 63103, USA.; Division of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., Bullingham OMN; Department of Integrative Biology, University of Guelph, ON, Canada, N1G 2W1., Gillis TE; Department of Integrative Biology, University of Guelph, ON, Canada, N1G 2W1., Warren DE; Department of Biology, Saint Louis University, St Louis, MO 63103, USA. |
| المصدر: | The Journal of experimental biology [J Exp Biol] 2021 Jul 15; Vol. 224 (14). Date of Electronic Publication: 2021 Jul 30. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company Of Biologists Limited Country of Publication: England NLM ID: 0243705 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9145 (Electronic) Linking ISSN: 00220949 NLM ISO Abbreviation: J Exp Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Company Of Biologists Limited Original Publication: London, Cambridge Univ. Press. |
| مواضيع طبية MeSH: | Turtles*, Acclimatization ; Animals ; Cold Temperature ; Hypoxia ; Proteome ; Proteomics |
| مستخلص: | Western painted turtles (Chrysemys picta bellii) are the most anoxia-tolerant tetrapod. Survival time improves at low temperature and during ontogeny, such that adults acclimated to 3°C survive far longer without oxygen than either warm-acclimated adults or cold-acclimated hatchlings. As protein synthesis is rapidly suppressed to save energy at the onset of anoxia exposure, this study tested the hypothesis that cold acclimation would evoke preparatory changes in protein expression to support enhanced anoxia survival in adult but not hatchling turtles. To test this, adult and hatchling turtles were acclimated to either 20°C (warm) or 3°C (cold) for 5 weeks, and then the heart ventricles were collected for quantitative proteomic analysis. The relative abundance of 1316 identified proteins was compared between temperatures and developmental stages. The effect of cold acclimation on the cardiac proteome was only evident in the context of an interaction with life stage, suggesting that ontogenic differences in anoxia tolerance may be predicated on successful maturation of the heart. The main differences between the hatchling and adult cardiac proteomes reflect an increase in metabolic scope with age that included more myoglobin and increased investment in both aerobic and anaerobic energy pathways. Mitochondrial structure and function were key targets of the life stage- and temperature-induced changes to the cardiac proteome, including reduced Complex II proteins in cold-acclimated adults that may help down-regulate the electron transport system and avoid succinate accumulation during anoxia. Therefore, targeted cold-induced changes to the cardiac proteome may be a contributing mechanism for stage-specific anoxia tolerance in turtles. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2021. Published by The Company of Biologists Ltd.) |
| معلومات مُعتمدة: | National Science Foundation CAREER; Natural Sciences and Engineering Research Council of Canada |
| فهرسة مساهمة: | Keywords: Development; Ectotherm; Hatchling; Heart; Protein expression; Temperature; iTRAQ |
| المشرفين على المادة: | 0 (Proteome) |
| تواريخ الأحداث: | Date Created: 20210730 Date Completed: 20210806 Latest Revision: 20230622 |
| رمز التحديث: | 20230623 |
| DOI: | 10.1242/jeb.242387 |
| PMID: | 34328184 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9145 |
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| DOI: | 10.1242/jeb.242387 |