دورية أكاديمية
Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy.
| العنوان: | Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy. |
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| المؤلفون: | Fredrickson SJ; Department of Biology, Missouri State University, Springfield, MO, USA., Hoog TG; Department of Biology, Missouri State University, Springfield, MO, USA., Udan RS; Department of Biology, Missouri State University, Springfield, MO, USA. RyanUdan@MissouriState.edu. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2319, pp. 93-104. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Embryonic Development* , Neovascularization, Physiologic*, Angiography/*methods , Blood Vessels/*diagnostic imaging , Culture Techniques/*methods , Embryo, Mammalian/*diagnostic imaging , Microscopy, Fluorescence/*methods, Animals ; Blood Vessels/growth & development ; Blood Vessels/metabolism ; Culture Media/chemistry ; Dissection/methods ; Embryo, Mammalian/blood supply ; Mice ; Mice, Transgenic ; Microscopy, Confocal |
| مستخلص: | Lightsheet microscopy is a form of fluorescence microscopy that can be used to visualize specimen with high resolution, a large depth-of-field, and minimal photodamage and photobleaching as compared to traditional confocal microscopy. As this technology becomes much more readily available, it will be useful in revealing new findings in the cardiovascular development field that may be hidden or difficult to image. In this manuscript, we describe an approach for mounting and culturing postimplantation mouse embryos to visualize blood vessel development with a lightsheet microscope. (© 2021. Springer Science+Business Media, LLC, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: Angiogenesis; Blood vessel; Embryo culture; Lightsheet microscopy; Mouse; SPIM; Vasculogenesis |
| المشرفين على المادة: | 0 (Culture Media) |
| تواريخ الأحداث: | Date Created: 20210731 Date Completed: 20210816 Latest Revision: 20210816 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/978-1-0716-1480-8_11 |
| PMID: | 34331247 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-1480-8_11 |