التفاصيل البيبلوغرافية
| العنوان: |
Single-Cell Profiling of the Antigen-Specific Response to BNT162b2 SARS-CoV-2 RNA Vaccine. |
| المؤلفون: |
Kramer KJ, Wilfong EM, Voss K, Barone SM, Shiakolas AR, Raju N, Roe CE, Suryadevara N, Walker L, Wall SC, Paulo A, Schaefer S, Dahunsi D, Westlake CS, Crowe JE, Carnahan RH, Rathmell JC, Bonami RH, Georgiev IS, Irish JM |
| المصدر: |
BioRxiv : the preprint server for biology [bioRxiv] 2021 Jul 28. Date of Electronic Publication: 2021 Jul 28. |
| نوع المنشور: |
Preprint |
| اللغة: |
English |
| بيانات الدورية: |
Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: |
RNA-based vaccines against SARS-CoV-2 are critical to limiting COVID-19 severity and spread. Cellular mechanisms driving antigen-specific responses to these vaccines, however, remain uncertain. We used single-cell technologies to identify and characterized antigen-specific cells and antibody responses to the RNA vaccine BNT162b2 in longitudinal samples from a cohort of healthy donors. Mass cytometry and machine learning pinpointed a novel expanding, population of antigen-specific non-canonical memory CD4 + and CD8 + T cells. B cell sequencing suggested progression from IgM, with apparent cross-reactivity to endemic coronaviruses, to SARS-CoV-2-specific IgA and IgG memory B cells and plasmablasts. Responding lymphocyte populations correlated with eventual SARS-CoV-2 IgG and a donor lacking these cell populations failed to sustain SARS-CoV-2-specific antibodies and experienced breakthrough infection. These integrated proteomic and genomic platforms reveal an antigen-specific cellular basis of RNA vaccine-based immunity.
One Sentence Summary: Single-cell profiling reveals the cellular basis of the antigen-specific response to the BNT162b2 SARS-CoV-2 RNA vaccine. |
| التعليقات: |
Update in: Nat Commun. 2022 Jun 16;13(1):3466. (PMID: 35710908) |
| معلومات مُعتمدة: |
K12 HD043483 United States HD NICHD NIH HHS; KL2 TR002245 United States TR NCATS NIH HHS; R01 DK105550 United States DK NIDDK NIH HHS; T32 DK101003 United States DK NIDDK NIH HHS |
| تواريخ الأحداث: |
Date Created: 20210803 Latest Revision: 20221103 |
| رمز التحديث: |
20221213 |
| مُعرف محوري في PubMed: |
PMC8328055 |
| DOI: |
10.1101/2021.07.28.453981 |
| PMID: |
34341788 |
| قاعدة البيانات: |
MEDLINE |
