دورية أكاديمية
The atypical RNA-binding protein Taf15 regulates dorsoanterior neural development through diverse mechanisms in Xenopus tropicalis.
| العنوان: | The atypical RNA-binding protein Taf15 regulates dorsoanterior neural development through diverse mechanisms in Xenopus tropicalis. |
|---|---|
| المؤلفون: | DeJong CS; Molecular and Cell Biology Department, Genetics, Genomics and Development Division, University of California, Berkeley, CA 94720, USA., Dichmann DS; Molecular and Cell Biology Department, Genetics, Genomics and Development Division, University of California, Berkeley, CA 94720, USA., Exner CRT; Department of Psychiatry, Weill Institute for Neurosciences, Quantitative Biosciences Institute, University of California San Francisco, San Francisco, CA 94143, USA., Xu Y; Department of Psychiatry, Weill Institute for Neurosciences, Quantitative Biosciences Institute, University of California San Francisco, San Francisco, CA 94143, USA., Harland RM; Molecular and Cell Biology Department, Genetics, Genomics and Development Division, University of California, Berkeley, CA 94720, USA. |
| المصدر: | Development (Cambridge, England) [Development] 2021 Aug 01; Vol. 148 (15). Date of Electronic Publication: 2021 Aug 04. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company Of Biologists Limited Country of Publication: England NLM ID: 8701744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9129 (Electronic) Linking ISSN: 09501991 NLM ISO Abbreviation: Development Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge Eng : Company Of Biologists Limited Original Publication: [Cambridge] : Company of Biologists, [c1987- |
| مواضيع طبية MeSH: | Brain/*metabolism , Neurogenesis/*physiology , Neurons/*metabolism , RNA-Binding Proteins/*metabolism , TATA-Binding Protein Associated Factors/*metabolism , Xenopus/*metabolism, Animals ; Brain/physiology ; Cell Differentiation/physiology ; Exons/physiology ; Female ; Male ; Neurons/physiology ; Xenopus/physiology |
| مستخلص: | The FET family of atypical RNA-binding proteins includes Fused in sarcoma (FUS), Ewing's sarcoma (EWS) and the TATA-binding protein-associate factor 15 (TAF15). FET proteins are highly conserved, suggesting specialized requirements for each protein. Fus regulates splicing of transcripts required for mesoderm differentiation and cell adhesion in Xenopus, but the roles of Ews and Taf15 remain unknown. Here, we analyze the roles of maternally deposited and zygotically transcribed Taf15, which is essential for the correct development of dorsoanterior neural tissues. By measuring changes in exon usage and transcript abundance from Taf15-depleted embryos, we found that Taf15 may regulate dorsoanterior neural development through fgfr4 and ventx2.1. Taf15 uses distinct mechanisms to downregulate Fgfr4 expression, namely retention of a single intron within fgfr4 when maternal and zygotic Taf15 is depleted, and reduction in the total fgfr4 transcript when zygotic Taf15 alone is depleted. The two mechanisms of gene regulation (post-transcriptional versus transcriptional) suggest that Taf15-mediated gene regulation is target and co-factor dependent, contingent on the milieu of factors that are present at different stages of development. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2021. Published by The Company of Biologists Ltd.) |
| References: | Mol Cell. 2009 Oct 23;36(2):178-91. (PMID: 19854129) Development. 2001 Jun;128(12):2407-20. (PMID: 11493559) J Mol Cell Biol. 2009 Dec;1(2):82-92. (PMID: 19783543) Exp Cell Res. 2007 Dec 10;313(20):4196-207. (PMID: 17765891) J Biol Chem. 2000 Nov 3;275(44):34365-74. (PMID: 10938274) EMBO J. 2016 May 17;35(10):1077-97. (PMID: 26951610) Sarcoma. 2011;2011:837474. (PMID: 21197473) Development. 2014 Apr;141(8):1683-93. (PMID: 24715458) Genes Dev. 2012 Jun 15;26(12):1351-63. (PMID: 22713872) J Clin Invest. 2007 May;117(5):1314-23. (PMID: 17415412) Cell. 2011 Sep 2;146(5):720-31. (PMID: 21884934) Prog Neurobiol. 2001 Oct;65(3):289-308. (PMID: 11473790) Cancer Res. 2002 Oct 1;62(19):5408-12. (PMID: 12359745) Dev Biol. 1999 Dec 15;216(2):561-81. (PMID: 10642793) RNA. 2009 Oct;15(10):1896-908. (PMID: 19656867) Brain Res. 2012 Jun 26;1462:61-80. (PMID: 22445064) Dev Dyn. 2002 Dec;225(4):499-510. (PMID: 12454926) Dev Dyn. 2008 Jul;237(7):1755-66. (PMID: 18521943) Science. 2009 Feb 27;323(5918):1208-1211. (PMID: 19251628) Dev Biol. 2000 Jun 1;222(1):124-34. (PMID: 10885751) Dev Cell. 2005 Mar;8(3):401-11. (PMID: 15737935) J Cell Biol. 2011 Oct 3;195(1):27-40. (PMID: 21949414) Dev Biol. 2019 Aug 1;452(1):8-20. (PMID: 30980799) Oncogene. 1999 Dec 9;18(52):7594-8. (PMID: 10602519) Nat Struct Mol Biol. 2011 Nov 13;18(12):1428-31. (PMID: 22081015) Genes Dev. 2015 Jan 1;29(1):63-80. (PMID: 25561496) Cell. 1994 Dec 2;79(5):791-803. (PMID: 7528101) Genome Biol. 2014;15(12):550. (PMID: 25516281) Nat Protoc. 2012 Mar 01;7(3):562-78. (PMID: 22383036) Development. 2014 Oct;141(19):3740-51. (PMID: 25209246) Cell Rep. 2013 Nov 27;5(4):918-25. (PMID: 24268778) Nature. 1993 Jun 17;363(6430):640-4. (PMID: 8510758) Annu Rev Biochem. 2003;72:291-336. (PMID: 12626338) Nature. 1992 Sep 10;359(6391):162-5. (PMID: 1522903) Nat Genet. 1993 Jun;4(2):175-80. (PMID: 7503811) Cancer Res. 1999 Oct 15;59(20):5064-7. (PMID: 10537274) Development. 1998 Apr;125(8):1447-56. (PMID: 9502725) BMC Cell Biol. 2008 Jul 11;9:37. (PMID: 18620564) RNA Biol. 2016 Nov;13(11):1089-1102. (PMID: 27415968) Development. 2005 Aug;132(15):3381-92. (PMID: 15975940) Oncogene. 2013 Sep 26;32(39):4646-55. (PMID: 23128393) Cell. 2012 May 11;149(4):753-67. (PMID: 22579281) EMBO J. 2007 Jun 20;26(12):2955-65. (PMID: 17525737) Brain. 2011 Sep;134(Pt 9):2595-609. (PMID: 21856723) Proc Natl Acad Sci U S A. 2010 Sep 28;107(39):16946-51. (PMID: 20833819) Biochem Biophys Res Commun. 2009 Jul 10;384(4):495-500. (PMID: 19426707) Nat Commun. 2016 Feb 04;7:10465. (PMID: 26842965) Annu Rev Biochem. 2015;84:355-79. (PMID: 25494299) Nat Commun. 2016 Jul 05;7:12143. (PMID: 27378374) Nature. 2004 Nov 11;432(7014):235-40. (PMID: 15531877) Wiley Interdiscip Rev RNA. 2011 Sep-Oct;2(5):700-17. (PMID: 21823230) Nat Genet. 2000 Feb;24(2):175-9. (PMID: 10655065) |
| معلومات مُعتمدة: | R01 GM042341 United States GM NIGMS NIH HHS; R35 GM127069 United States GM NIGMS NIH HHS; U01 MH115747 United States MH NIMH NIH HHS |
| فهرسة مساهمة: | Keywords: Xenopus; Embryo development; FET proteins; Maternal deposition; RNA-seq; Transcript regulation |
| المشرفين على المادة: | 0 (RNA-Binding Proteins) 0 (TATA-Binding Protein Associated Factors) |
| تواريخ الأحداث: | Date Created: 20210804 Date Completed: 20211012 Latest Revision: 20220805 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8353262 |
| DOI: | 10.1242/dev.191619 |
| PMID: | 34345915 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9129 |
|---|---|
| DOI: | 10.1242/dev.191619 |