دورية أكاديمية
أعرض في EDS

Antibody interference by a non-neutralizing antibody abrogates humoral protection against Plasmodium yoelii liver stage.

التفاصيل البيبلوغرافية
العنوان: Antibody interference by a non-neutralizing antibody abrogates humoral protection against Plasmodium yoelii liver stage.
المؤلفون: Vijayan K; Seattle Children's Research Institute, Seattle, WA, USA., Visweswaran GRR; Seattle Children's Research Institute, Seattle, WA, USA., Chandrasekaran R; Seattle Children's Research Institute, Seattle, WA, USA., Trakhimets O; Seattle Children's Research Institute, Seattle, WA, USA., Brown SL; Seattle Children's Research Institute, Seattle, WA, USA., Watson A; Seattle Children's Research Institute, Seattle, WA, USA., Zuck M; Seattle Children's Research Institute, Seattle, WA, USA., Dambrauskas N; Seattle Children's Research Institute, Seattle, WA, USA., Raappana A; Seattle Children's Research Institute, Seattle, WA, USA., Carbonetti S; Seattle Children's Research Institute, Seattle, WA, USA., Kelnhofer-Millevolte L; MSTP program, University of Washington, Seattle, WA, USA., Glennon EKK; Seattle Children's Research Institute, Seattle, WA, USA., Postiglione R; Seattle Children's Research Institute, Seattle, WA, USA., Sather DN; Seattle Children's Research Institute, Seattle, WA, USA; Department of Pediatrics, University of Washington, Seattle, WA, USA; Department of Global Health, University of Washington, Seattle, WA, USA. Electronic address: noah.sather@seattlechildrens.org., Kaushansky A; Seattle Children's Research Institute, Seattle, WA, USA; Department of Pediatrics, University of Washington, Seattle, WA, USA; Department of Global Health, University of Washington, Seattle, WA, USA; Brotman Baty Research Institute, Seattle, WA, USA; Institute for Stem Cell and Regenerative Medicine, Seattle, WA, USA. Electronic address: alexis.kaushansky@seattlechildrens.org.
المصدر: Cell reports [Cell Rep] 2021 Aug 03; Vol. 36 (5), pp. 109489.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Cambridge, MA] : Cell Press, c 2012-
مواضيع طبية MeSH: Immunity, Humoral* , Life Cycle Stages*, Antibodies, Neutralizing/*immunology , Antibodies, Protozoan/*immunology , Liver/*parasitology , Plasmodium yoelii/*growth & development , Plasmodium yoelii/*immunology, Animals ; Antibodies, Monoclonal/immunology ; Antibodies, Monoclonal/isolation & purification ; Cell Line ; Epitopes/immunology ; Female ; Kinetics ; Malaria Vaccines/immunology ; Mice, Inbred BALB C ; Models, Biological ; Protein Binding ; Protein Domains ; Protozoan Proteins/chemistry ; Protozoan Proteins/immunology ; Sporozoites/immunology ; Vaccines, Synthetic/immunology ; Mice
مستخلص: Both subunit and attenuated whole-sporozoite vaccination strategies against Plasmodium infection have shown promising initial results in malaria-naive westerners but less efficacy in malaria-exposed individuals in endemic areas. Here, we demonstrate proof of concept by using a rodent malaria model in which non-neutralizing antibodies (nNAbs) can directly interfere with protective anti-circumsporozoite protein (CSP) humoral responses. We characterize a monoclonal antibody, RAM1, against Plasmodium yoelii sporozoite major surface antigen CSP. Unlike the canonical PyCSP repeat domain binding and neutralizing antibody (NAb) 2F6, RAM1 does not inhibit sporozoite traversal or entry of hepatocytes in vitro or infection in vivo. Although 2F6 and RAM1 bind non-overlapping regions of the CSP-repeat domain, pre-treatment with RAM1 abrogates the capacity of NAb to block sporozoite traversal and invasion in vitro. Importantly, RAM1 reduces the efficacy of the polyclonal humoral response against PyCSP in vivo. Collectively, our data provide a proof of concept that nNAbs can alter the efficacy of malaria vaccination.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: B cell; Plasmodium; antigen; circumsporozoite protein; humoral immunity; malaria; neutralizing antibodies; pre-existing immunity; repeat region; vaccines
المشرفين على المادة: 0 (Antibodies, Monoclonal)
0 (Antibodies, Neutralizing)
0 (Antibodies, Protozoan)
0 (Epitopes)
0 (Malaria Vaccines)
0 (Protozoan Proteins)
0 (Vaccines, Synthetic)
تواريخ الأحداث: Date Created: 20210804 Date Completed: 20220211 Latest Revision: 20240226
رمز التحديث: 20240226
DOI: 10.1016/j.celrep.2021.109489
PMID: 34348141
قاعدة البيانات: MEDLINE
الوصف
تدمد:2211-1247
DOI:10.1016/j.celrep.2021.109489