دورية أكاديمية
أعرض في EDS

Mechanism of preservation of the intestinal mucosa architecture and NF-κB/PGE2 reduction by hydrogen sulfide on cholera toxin-induced diarrhea in mice.

التفاصيل البيبلوغرافية
العنوان: Mechanism of preservation of the intestinal mucosa architecture and NF-κB/PGE2 reduction by hydrogen sulfide on cholera toxin-induced diarrhea in mice.
المؤلفون: Sousa FBM; Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (LAFIDG), Post-graduation Program in Biotechnology, Parnaiba Delta Federal University, Parnaíba, PI, Brazil; Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina, PI, Brazil., Pacheco G; Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (LAFIDG), Post-graduation Program in Biotechnology, Parnaiba Delta Federal University, Parnaíba, PI, Brazil., Oliveira AP; Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (LAFIDG), Post-graduation Program in Biotechnology, Parnaiba Delta Federal University, Parnaíba, PI, Brazil; Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina, PI, Brazil., Nicolau LAD; Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (LAFIDG), Post-graduation Program in Biotechnology, Parnaiba Delta Federal University, Parnaíba, PI, Brazil., Lopes ALF; Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (LAFIDG), Post-graduation Program in Biotechnology, Parnaiba Delta Federal University, Parnaíba, PI, Brazil., Ferreira-Fernandes H; Laboratory of Genetics and Molecular Biology, Parnaiba Delta Federal University, Parnaíba, PI, Brazil., Pinto GR; Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina, PI, Brazil; Laboratory of Genetics and Molecular Biology, Parnaiba Delta Federal University, Parnaíba, PI, Brazil., Medeiros JVR; Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (LAFIDG), Post-graduation Program in Biotechnology, Parnaiba Delta Federal University, Parnaíba, PI, Brazil; Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina, PI, Brazil. Electronic address: jandvenes@ufpi.edu.br.
المصدر: Life sciences [Life Sci] 2021 Nov 01; Vol. 284, pp. 119869. Date of Electronic Publication: 2021 Aug 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: <2008->: Amsterdam : Elsevier
Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press.
مواضيع طبية MeSH: Diarrhea/*chemically induced , Diarrhea/*metabolism , Dinoprostone/*metabolism , Hydrogen Sulfide/*pharmacology , Intestinal Mucosa/*pathology , NF-kappa B/*metabolism, Animals ; Cholera Toxin ; Female ; Gene Expression Profiling ; Male ; Mice ; Morpholines/pharmacology ; Organothiophosphorus Compounds/pharmacology ; Receptors, Prostaglandin E, EP2 Subtype/metabolism ; Receptors, Prostaglandin E, EP4 Subtype/metabolism
مستخلص: Aims: Investigate the involvement of Hydrogen sulfide (H 2 S) in inflammatory parameters and intestinal morphology caused by cholera toxin (CT) in mice.
Main Methods: Mice were subjected to the procedure of inducing diarrhea by CT in the isolated intestinal loop model. The intestinal loops were inoculated with H 2 S donor molecules (NaHS and GYY 4137) or saline and CT. To study the role of EP2 and EP4 prostaglandin E2 (PGE2) receptors in the H 2 S antisecretory effect, PAG (DL-propargylglycine - inhibitor of cystathionine-γ-lyase (CSE)), PF-04418948 (EP2 antagonist) and ONO-AE3-208 (EP4 antagonist) were used. The intestinal loops were evaluated for intestinal secretion, relation of the depth of villi and intestinal crypts, and real-time PCR for the mRNA of the CXCL2, IL-6, NOS-2, IL-17, NF-κB1, NF-κBIA, SLC6A4 and IFN-γ genes.
Key Findings: H 2 S restored the villus/crypt depth ratio caused by CT. NaHS and GYY 4137 increased the expression of NF-κB1 and for the NF-κBIA gene, only GYY 4137 increased the expression of this gene. The increased expression of NF-κB inhibitors, NF-κB1 and NF-κBIA by H 2 S indicates a possible decrease in NF-κB activity. The pretreatment with PAG reversed the protective effect of PF-04418948 and ONO-AE3-208, indicating that H 2 S probably decreases PGE2 because in the presence of antagonists of this pathway, PAG promotes intestinal secretion.
Significance: Our results point to a protective activity of H 2 S against CT for promoting a protection of villus and crypt intestine morphology and also that its mechanism occurs at least in part due to decreasing the activity of NF-κB and PGE2.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Cholera; Hydrogen sulfide; Intestinal morphometry; NF-κB
المشرفين على المادة: 0 (GYY 4137)
0 (Morpholines)
0 (NF-kappa B)
0 (Organothiophosphorus Compounds)
0 (Receptors, Prostaglandin E, EP2 Subtype)
0 (Receptors, Prostaglandin E, EP4 Subtype)
9012-63-9 (Cholera Toxin)
K7Q1JQR04M (Dinoprostone)
YY9FVM7NSN (Hydrogen Sulfide)
تواريخ الأحداث: Date Created: 20210806 Date Completed: 20211007 Latest Revision: 20211007
رمز التحديث: 20221213
DOI: 10.1016/j.lfs.2021.119869
PMID: 34358552
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0631
DOI:10.1016/j.lfs.2021.119869