دورية أكاديمية
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Chondro-inductive hyaluronic acid/chitosan coacervate-based scaffolds for cartilage tissue engineering.

التفاصيل البيبلوغرافية
العنوان: Chondro-inductive hyaluronic acid/chitosan coacervate-based scaffolds for cartilage tissue engineering.
المؤلفون: Karabıyık Acar Ö; Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey. Electronic address: ozge.acar@hotmail.com., Bedir S; Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey., Kayitmazer AB; Department of Chemistry, Bogazici University, Bebek, Istanbul, Turkey., Kose GT; Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey. Electronic address: gamzekose@yeditepe.edu.tr.
المصدر: International journal of biological macromolecules [Int J Biol Macromol] 2021 Oct 01; Vol. 188, pp. 300-312. Date of Electronic Publication: 2021 Aug 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
مواضيع طبية MeSH: Tissue Engineering*, Cell Differentiation/*drug effects , Chitosan/*pharmacology , Chondrogenesis/*drug effects, Animals ; Cartilage, Articular/drug effects ; Cartilage, Articular/growth & development ; Chitosan/chemistry ; Hyaluronic Acid/chemistry ; Hyaluronic Acid/pharmacology ; Mesenchymal Stem Cells/drug effects ; Rats ; Tissue Scaffolds/chemistry
مستخلص: Injuries related to articular cartilage are among the most challenging musculoskeletal problems because of poor repair capacity of this tissue. The lack of efficient treatments for chondral defects has stimulated research on cartilage tissue engineering applications combining porous biocompatible scaffolds with stem cells in the presence of external stimuli. This work presents the role of rat bone marrow mesenchymal stem cell (BMSC) encapsulated-novel three-dimensional (3D) coacervate scaffolds prepared through complex coacervation between different chitosan salts (CHI) and sodium hyaluronate (HA). The 3D architecture of BMSC encapsulated scaffolds (HA/CHI) was shown by scanning electron microscopy (SEM) to have an interconnected structure to allow cell-cell and cell-matrix interactions. Chondrogenic induction of encapsulated BMSCs within HA/CHI coacervates demonstrated remarkable cellular viability in addition to the elevated expression levels of chondrogenic markers such as sex determining region Y-box 9 protein (SOX9), aggrecan (ACAN), cartilage oligomeric matrix protein (COMP) and collagen type II (COL2A1) by immunofluorescence staining, qPCR and ELISA test. Collectively, HA/CHI coacervates are promising candidates for future use of these scaffolds in cartilage tissue engineering applications.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Bone marrow mesenchymal stem cell; Cartilage tissue engineering; Chitosan; Complex coacervation; Encapsulation; Sodium hyaluronate
المشرفين على المادة: 9004-61-9 (Hyaluronic Acid)
9012-76-4 (Chitosan)
تواريخ الأحداث: Date Created: 20210806 Date Completed: 20211217 Latest Revision: 20211217
رمز التحديث: 20221213
DOI: 10.1016/j.ijbiomac.2021.07.176
PMID: 34358603
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0003
DOI:10.1016/j.ijbiomac.2021.07.176