دورية أكاديمية
أعرض في EDS

Raman needle arthroscopy for in vivo molecular assessment of cartilage.

التفاصيل البيبلوغرافية
العنوان: Raman needle arthroscopy for in vivo molecular assessment of cartilage.
المؤلفون: Kroupa KR; Department of Mechanical Engineering, Boston University, Boston, Massachusetts, USA., Wu MI; Department of Mechanical Engineering, Boston University, Boston, Massachusetts, USA., Zhang J; Department of Biomedical Engineering, Boston University, Boston, Massachusetts, USA., Jensen M; Department of Craniofacial Development & Stem Cell Biology, Kings College, London, UK., Wong W; Department of Mechanical Engineering, Boston University, Boston, Massachusetts, USA., Engiles JB; Department of Pathobiology, New Bolton Center, University of Pennsylvania, Kennett Square, Pennsylvania, USA., Schaer TP; Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Grinstaff MW; Department of Biomedical Engineering, Boston University, Boston, Massachusetts, USA.; Division of Materials Science & Engineering, Boston University, Boston, Massachusetts, USA., Snyder BD; Department of Orthopaedic Surgery, Boston Children's Hospital, Boston, Massachusetts, USA., Bergholt MS; Department of Craniofacial Development & Stem Cell Biology, Kings College, London, UK., Albro MB; Department of Mechanical Engineering, Boston University, Boston, Massachusetts, USA.; Division of Materials Science & Engineering, Boston University, Boston, Massachusetts, USA.
المصدر: Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2022 Jun; Vol. 40 (6), pp. 1338-1348. Date of Electronic Publication: 2021 Aug 18.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: United States NLM ID: 8404726 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-527X (Electronic) Linking ISSN: 07360266 NLM ISO Abbreviation: J Orthop Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 2006- : Hoboken, NJ : Wiley
Original Publication: New York, N.Y. : Raven Press, [c1983-
مواضيع طبية MeSH: Cartilage, Articular*/chemistry , Osteoarthritis*, Animals ; Arthroscopy ; Cattle ; Collagen/analysis ; Glycosaminoglycans/analysis ; Humans ; Sheep
مستخلص: The development of treatments for osteoarthritis (OA) is burdened by the lack of standardized biomarkers of cartilage health that can be applied in clinical trials. We present a novel arthroscopic Raman probe that can "optically biopsy" cartilage and quantify key extracellular matrix (ECM) biomarkers for determining cartilage composition, structure, and material properties in health and disease. Technological and analytical innovations to optimize Raman analysis include (1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H 2 O] scores), and (2) multiplexed polarized Raman spectroscopy to quantify superficial zone (SZ) COL anisotropy via a partial least squares-discriminant analysis-derived Raman collagen alignment factor (RCAF). Raman measurements were performed on a series of ex vivo cartilage models: (1) chemically GAG-depleted bovine cartilage explants (n = 40), (2) mechanically abraded bovine cartilage explants (n = 30), (3) aging human cartilage explants (n = 14), and (4) anatomical-site-varied ovine osteochondral explants (n = 6). Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p < 0.001). RCAF values were significantly different for explants with abrasion-induced SZ COL loss (p < 0.001). The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H 2 O scores) predicted 94% of the variation in elastic modulus of ovine explants (p < 0.001). Finally, we demonstrated the first in vivo Raman arthroscopy assessment of an ovine femoral condyle through intraarticular entry into the synovial capsule. This study advances Raman arthroscopy toward a transformative low-cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes from emerging OA therapies.
(© 2021 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals LLC on behalf of Orthopaedic Research Society.)
References: Arthritis Rheumatol. 2014 Jul;66(7):1820-31. (PMID: 24740822)
Ann Rheum Dis. 1977 Oct;36(5):399-406. (PMID: 200188)
Arthritis Rheum. 2005 Nov;52(11):3507-14. (PMID: 16258919)
J Orthop Res. 2022 Jun;40(6):1338-1348. (PMID: 34370873)
BMC Musculoskelet Disord. 2017 Feb 22;18(1):91. (PMID: 28228115)
J Biomed Opt. 2011 Jan-Feb;16(1):017003. (PMID: 21280924)
Magn Reson Imaging. 2009 Jul;27(6):779-84. (PMID: 19269769)
J Biophotonics. 2018 Apr;11(4):e201700217. (PMID: 29227045)
Appl Spectrosc. 2004 Apr;58(4):376-81. (PMID: 15104805)
Opt Lett. 2020 May 15;45(10):2890-2893. (PMID: 32412494)
Osteoarthritis Cartilage. 2019 May;27(5):810-822. (PMID: 30660720)
NPJ Regen Med. 2018 Feb 9;3:3. (PMID: 29449966)
Anal Bioanal Chem. 2015 Oct;407(26):8067-77. (PMID: 26319282)
Osteoarthritis Cartilage. 2019 Feb;27(2):304-313. (PMID: 30359723)
Lancet. 2019 Apr 27;393(10182):1745-1759. (PMID: 31034380)
Magn Reson Imaging. 2011 Apr;29(3):324-34. (PMID: 21130590)
Osteoarthritis Cartilage. 2006 Jan;14(1):13-29. (PMID: 16242352)
Anal Chim Acta. 2016 Jul 5;926:79-87. (PMID: 27216396)
J Orthop Res. 2011 May;29(5):704-9. (PMID: 21437949)
Biomaterials. 2016 Aug;98:1-22. (PMID: 27177218)
J Mech Behav Biomed Mater. 2016 Mar;56:106-119. (PMID: 26700573)
J Mech Behav Biomed Mater. 2014 Mar;31:77-85. (PMID: 23545201)
J Cell Physiol. 2007 Dec;213(3):626-34. (PMID: 17786965)
Magn Reson Med. 1999 May;41(5):857-65. (PMID: 10332865)
J Orthop Res. 2004 May;22(3):565-70. (PMID: 15099636)
Osteoarthritis Cartilage. 2009 Nov;17(11):1469-76. (PMID: 19464244)
Biomaterials. 2017 Sep;140:128-137. (PMID: 28649013)
Appl Spectrosc. 2006 Oct;60(10):1134-41. (PMID: 17059665)
Orthop Res Rev. 2018 Oct 23;10:73-81. (PMID: 30774462)
Osteoarthritis Cartilage. 2010 Oct;18 Suppl 3:S93-105. (PMID: 20864027)
Clin J Am Soc Nephrol. 2009 Feb;4(2):461-9. (PMID: 19201920)
Osteoarthritis Cartilage. 2019 Aug;27(8):1235-1243. (PMID: 31026649)
Osteoarthritis Cartilage. 2012 May;20(5):396-404. (PMID: 22334095)
Ann Rheum Dis. 1977 Apr;36(2):121-9. (PMID: 856064)
Arthritis Rheum. 2008 Jan;58(1):26-35. (PMID: 18163497)
Analyst. 2011 Apr 21;136(8):1675-85. (PMID: 21359366)
Lasers Med Sci. 2014 Mar;29(2):797-804. (PMID: 23979802)
J Orthop Res. 2021 Apr;39(4):780-787. (PMID: 32833239)
Ann Rheum Dis. 2011 Oct;70(10):1835-7. (PMID: 21742640)
Scand J Work Environ Health. 1996 Jun;22(3):165-75. (PMID: 8837261)
فهرسة مساهمة: Keywords: Raman spectroscopy; articular cartilage; imaging; osteoarthritis diagnostics
المشرفين على المادة: 0 (Glycosaminoglycans)
9007-34-5 (Collagen)
تواريخ الأحداث: Date Created: 20210809 Date Completed: 20220517 Latest Revision: 20220721
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9291802
DOI: 10.1002/jor.25155
PMID: 34370873
قاعدة البيانات: MEDLINE
الوصف
تدمد:1554-527X
DOI:10.1002/jor.25155