دورية أكاديمية
أعرض في EDS

Mesenchymal stem cell-derived extracellular vesicles ameliorate Alzheimer's disease-like phenotypes in a preclinical mouse model.

التفاصيل البيبلوغرافية
العنوان: Mesenchymal stem cell-derived extracellular vesicles ameliorate Alzheimer's disease-like phenotypes in a preclinical mouse model.
المؤلفون: Cone AS; Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL 32306, USA., Yuan X; Department of Chemical and Biomedical Engineering, Florida A&M University and Florida State University College of Engineering, Tallahassee, FL 32306, USA.; The National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida 32310, USA., Sun L; Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL 32306, USA., Duke LC; Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL 32306, USA., Vreones MP; Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL 32306, USA., Carrier AN; Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL 32306, USA., Kenyon SM; Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL 32306, USA., Carver SR; Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL 32306, USA., Benthem SD; Department of Neuroscience, Florida State University College of Psychology, Tallahassee, FL 32306, USA., Stimmell AC; Department of Neuroscience, Florida State University College of Psychology, Tallahassee, FL 32306, USA., Moseley SC; The National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida 32310, USA., Hike D; Department of Chemical and Biomedical Engineering, Florida A&M University and Florida State University College of Engineering, Tallahassee, FL 32306, USA.; The National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida 32310, USA., Grant SC; Department of Chemical and Biomedical Engineering, Florida A&M University and Florida State University College of Engineering, Tallahassee, FL 32306, USA.; The National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida 32310, USA., Wilber AA; Department of Neuroscience, Florida State University College of Psychology, Tallahassee, FL 32306, USA., Olcese JM; Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL 32306, USA., Meckes DG Jr; Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL 32306, USA.
المصدر: Theranostics [Theranostics] 2021 Jul 13; Vol. 11 (17), pp. 8129-8142. Date of Electronic Publication: 2021 Jul 13 (Print Publication: 2021).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Ivyspring International Publisher Country of Publication: Australia NLM ID: 101552395 Publication Model: eCollection Cited Medium: Internet ISSN: 1838-7640 (Electronic) Linking ISSN: 18387640 NLM ISO Abbreviation: Theranostics Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Wyoming, N.S.W. : Ivyspring International Publisher, 2011-
مواضيع طبية MeSH: Mesenchymal Stem Cell Transplantation*, Alzheimer Disease/*therapy, Amyloid beta-Peptides/metabolism ; Animals ; Brain/metabolism ; Disease Models, Animal ; Extracellular Vesicles/metabolism ; Hippocampus/metabolism ; Immunomodulation ; Mesenchymal Stem Cells/metabolism ; Mice ; Mice, Transgenic ; Plaque, Amyloid/metabolism
مستخلص: Alzheimer's disease (AD) is an irreversible neurodegenerative disorder that affects more than 44 million people worldwide. Despite the high disease burden, there is no effective treatment for people suffering from AD. Mesenchymal stem cells (MSCs) are multipotent stromal cells that have been widely studied due to their therapeutic potential. However, administration of cells has been found to have a multitude of limitations. Recently, extracellular vesicles (EVs) derived from MSCs have been studied as a therapeutic candidate, as they exhibit similar immunoprotective and immunomodulatory abilities as the host human MSCs. Methods: To test the potential therapeutic effects of MSC EVs, human bone-marrow derived MSCs were grown in three-dimensional (3D) cell culture, and small EVs were harvested using differential ultracentrifugation. These small EVs were given to non-transgenic (NT) or 5XFAD (5 familial Alzheimer's disease mutations) mice intranasally (IN) every 4 days for 4 months. The mice were then required to perform a variety of behavioral assays to measure changes in learning and memory. Afterwards, immunohistochemistry was performed on brain slices to measure amyloid beta (Aβ) and glial fibrillary acidic protein (GFAP) levels. Results: The data revealed that 5XFAD mice that received hMSC-EV treatment behaved significantly better in cognitive tests than saline treated 5XFAD mice, with no significant change between EV-treated 5XFAD mice and NT mice. Additionally, we found lower Aβ plaque load in the hippocampus of the EV-treated mice. Finally, less colocalization between GFAP and Aβ plaques was found in the brain of EV-treated mice compared to saline. Conclusions: Taken together, these data suggest that IN administration of MSC-derived EVs can slow down AD pathogenesis.
Competing Interests: Competing Interests: The authors have declared that no competing interest exists.
(© The author(s).)
References: J Neurosci. 1993 Apr;13(4):1676-87. (PMID: 8463843)
Front Cell Neurosci. 2018 Sep 24;12:317. (PMID: 30319358)
Psychiatry Clin Neurosci. 1998 Dec;52(6):593-9. (PMID: 9895207)
Trends Cell Biol. 2017 Mar;27(3):172-188. (PMID: 27979573)
Acta Biomater. 2020 Feb;103:196-212. (PMID: 31857259)
Clin Chim Acta. 1986 Mar 16;155(2):143-50. (PMID: 2421949)
Prog Neurobiol. 2011 Mar;93(3):421-43. (PMID: 21219963)
Int J Mol Sci. 2019 Nov 02;20(21):. (PMID: 31684046)
Brain Res Bull. 2018 Mar;137:285-293. (PMID: 29288735)
Cell Mol Life Sci. 2017 Jul;74(13):2345-2360. (PMID: 28214990)
Biomark Res. 2019 Apr 4;7:8. (PMID: 30992990)
Bio Protoc. 2018 Mar 5;8(5):. (PMID: 29651452)
J Alzheimers Dis. 2015;46(1):55-61. (PMID: 25720409)
Neurobiol Aging. 2000 May-Jun;21(3):383-421. (PMID: 10858586)
Neurobiol Aging. 2013 Apr;34(4):1051-9. (PMID: 23062700)
Stem Cells. 2015 Nov;33(11):3368-81. (PMID: 26274841)
Nature. 2016 Dec 7;540(7632):230-235. (PMID: 27929004)
Nat Rev Neurosci. 2015 Jun;16(6):358-72. (PMID: 25991443)
Curr Alzheimer Res. 2011 Mar;8(2):164-74. (PMID: 21345170)
J Extracell Vesicles. 2019 Apr 29;8(1):1609206. (PMID: 31069028)
Cells. 2019 Jul 28;8(8):. (PMID: 31357692)
Sci Rep. 2020 Jul 1;10(1):10772. (PMID: 32612165)
Brain. 2020 Sep 1;143(9):2803-2817. (PMID: 32812023)
J Vis Exp. 2012 Jan 09;(59):e3037. (PMID: 22257828)
Sci Rep. 2016 Apr 12;6:23978. (PMID: 27068479)
Neurobiol Dis. 2000 Dec;7(6 Pt B):682-9. (PMID: 11114266)
Nat Neurosci. 2012 Jun;15(6):862-70. (PMID: 22610068)
J Extracell Vesicles. 2018 Oct 17;7(1):1528109. (PMID: 30357008)
J Cell Mol Med. 2018 Mar;22(3):1428-1442. (PMID: 29392844)
Int J Mol Sci. 2019 Jul 31;20(15):. (PMID: 31370159)
Nat Cell Biol. 2012 Jun 03;14(7):677-85. (PMID: 22660413)
Front Neurol. 2013 Jul 01;4:83. (PMID: 23847585)
J Pharmacol Sci. 2020 Aug;143(4):290-299. (PMID: 32507685)
J Neuroinflammation. 2006 Sep 27;3:27. (PMID: 17005052)
Methods Mol Biol. 2019;1916:105-111. (PMID: 30535688)
J Neurochem. 2002 Nov;83(4):973-83. (PMID: 12421370)
J Neurosci Methods. 2018 Sep 1;307:210-220. (PMID: 29894726)
Leukemia. 2014 Apr;28(4):970-3. (PMID: 24445866)
Pathol Int. 1998 May;48(5):332-40. (PMID: 9704339)
Aging (Albany NY). 2020 Mar 25;12(6):5469-5478. (PMID: 32209731)
Cell. 2016 Mar 10;164(6):1226-1232. (PMID: 26967288)
Nat Methods. 2017 Feb 28;14(3):228-232. (PMID: 28245209)
Lancet Neurol. 2011 Sep;10(9):819-28. (PMID: 21775213)
BMC Mol Cell Biol. 2020 Jul 30;21(1):58. (PMID: 32731849)
J Cell Sci. 2004 Nov 15;117(Pt 24):5721-9. (PMID: 15537830)
Neurosci Lett. 1993 Mar 5;151(1):71-3. (PMID: 8469439)
Nat Rev Neurosci. 2007 Jan;8(1):57-69. (PMID: 17180163)
J Vis Exp. 2013 Apr 08;(74):. (PMID: 23608783)
J Cell Biol. 2018 Feb 5;217(2):459-472. (PMID: 29196460)
Nat Genet. 1992 Aug;1(5):345-7. (PMID: 1302033)
Science. 2020 Feb 7;367(6478):. (PMID: 32029601)
J Alzheimers Dis. 2018;61(3):1005-1013. (PMID: 29254100)
Trends Neurosci. 2009 Dec;32(12):638-47. (PMID: 19782411)
J Vis Exp. 2017 Aug 30;(126):. (PMID: 28892027)
Gene. 2019 Jul 15;705:157-166. (PMID: 31029603)
mBio. 2020 Jun 16;11(3):. (PMID: 32546618)
J Neurosci. 2006 Oct 4;26(40):10129-40. (PMID: 17021169)
Nat Rev Immunol. 2014 Jul;14(7):463-77. (PMID: 24962261)
Open Biol. 2017 Dec;7(12):. (PMID: 29237809)
J Neurosurg. 2009 Jun;110(6):1189-97. (PMID: 19301973)
Front Aging. 2021 May;2:. (PMID: 34746919)
Acta Neuropathol. 2009 Oct;118(4):475-85. (PMID: 19513731)
Stem Cells Dev. 2017 May 1;26(9):617-631. (PMID: 28186467)
Stem Cells Transl Med. 2019 May;8(5):490-499. (PMID: 30706999)
J Alzheimers Dis. 2010;22(2):683-984. (PMID: 20847415)
Immun Ageing. 2019 May 13;16:10. (PMID: 31114624)
Curr Biol. 2020 Jul 6;30(13):2588-2601.e5. (PMID: 32470367)
J Alzheimers Dis. 2018;62(3):1261-1276. (PMID: 29562537)
Sci Adv. 2020 Jul 22;6(30):eaba6884. (PMID: 32832666)
World J Stem Cells. 2020 Feb 26;12(2):100-109. (PMID: 32184935)
Stem Cells. 2017 Feb;35(2):398-410. (PMID: 27726254)
Mol Neurodegener. 2015 Jul 11;10:27. (PMID: 26159703)
J Extracell Vesicles. 2018 Nov 23;7(1):1535750. (PMID: 30637094)
Nat Cell Biol. 2019 Jan;21(1):9-17. (PMID: 30602770)
Clinics (Sao Paulo). 2011;66 Suppl 1:45-54. (PMID: 21779722)
Stem Cells Transl Med. 2020 Sep;9(9):1068-1084. (PMID: 32496649)
Biomed J. 2018 Feb;41(1):21-33. (PMID: 29673549)
Biomol Concepts. 2017 Mar 1;8(1):37-43. (PMID: 28231054)
Sci Rep. 2019 Feb 4;9(1):1311. (PMID: 30718609)
Cytotherapy. 2019 Oct;21(10):1033-1048. (PMID: 31537468)
Autophagy. 2014 Jan;10(1):32-44. (PMID: 24149893)
Cell Mol Neurobiol. 2016 Apr;36(3):301-12. (PMID: 27053351)
ScientificWorldJournal. 2015;2015:657086. (PMID: 25695100)
Mol Neurobiol. 2019 Dec;56(12):8628-8642. (PMID: 31297718)
Annu Rev Cell Dev Biol. 2014;30:255-89. (PMID: 25288114)
Commun Biol. 2020 Dec 15;3(1):774. (PMID: 33319867)
Brain Behav Immun. 2017 Mar;61:1-11. (PMID: 27395435)
Mol Autism. 2018 Nov 21;9:57. (PMID: 30479733)
Front Immunol. 2018 Dec 04;9:2837. (PMID: 30564236)
J Virol. 2017 Feb 14;91(5):. (PMID: 27974566)
J Neuroinflammation. 2006 Sep 07;3:24. (PMID: 16959029)
Neurobiol Aging. 1992 Sep-Oct;13(5):609-12. (PMID: 1461350)
Dis Model Mech. 2018 May 8;11(5):. (PMID: 29739861)
J Extracell Vesicles. 2016 Jul 13;5:31295. (PMID: 27421995)
معلومات مُعتمدة: R00 AG049090 United States AG NIA NIH HHS; R01 AG070094 United States AG NIA NIH HHS; R01 CA204621 United States CA NCI NIH HHS
فهرسة مساهمة: Keywords: Alzheimer's disease; exosomes; extracellular vesicles; mesenchymal stem cells; microvesicles
المشرفين على المادة: 0 (Amyloid beta-Peptides)
تواريخ الأحداث: Date Created: 20210810 Date Completed: 20220119 Latest Revision: 20231107
رمز التحديث: 20231107
مُعرف محوري في PubMed: PMC8344012
DOI: 10.7150/thno.62069
PMID: 34373732
قاعدة البيانات: MEDLINE
الوصف
تدمد:1838-7640
DOI:10.7150/thno.62069