دورية أكاديمية
Development of molecular driven screening for desulfurizing microorganisms targeting the dszB desulfinase gene.
| العنوان: | Development of molecular driven screening for desulfurizing microorganisms targeting the dszB desulfinase gene. |
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| المؤلفون: | Duval E; Université de Pau et des Pays de l'Adour, E2S UPPA, IPREM UMR, CNRS 5254, Bat. IBEAS, Pau, France; Segula Technologies, 71 rue Henri Gautier, 44550, Montoir de Bretagne, France. Electronic address: emmanuel.duval@univ-pau.fr., Cravo-Laureau C; Université de Pau et des Pays de l'Adour, E2S UPPA, IPREM UMR, CNRS 5254, Bat. IBEAS, Pau, France. Electronic address: cristiana.cravo-laureau@univ-pau.fr., Poinel L; Segula Technologies, 71 rue Henri Gautier, 44550, Montoir de Bretagne, France. Electronic address: line.poinel@segula.fr., Duran R; Université de Pau et des Pays de l'Adour, E2S UPPA, IPREM UMR, CNRS 5254, Bat. IBEAS, Pau, France. Electronic address: robert.duran@univ-pau.fr. |
| المصدر: | Research in microbiology [Res Microbiol] 2021 Sep-Oct; Vol. 172 (6), pp. 103872. Date of Electronic Publication: 2021 Aug 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: France NLM ID: 8907468 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1769-7123 (Electronic) Linking ISSN: 09232508 NLM ISO Abbreviation: Res Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Paris : Elsevier Original Publication: Amsterdam ; New York : Elsevier, [c1989- |
| مواضيع طبية MeSH: | Genes, Bacterial*, Actinobacteria/*enzymology , Bacterial Proteins/*genetics , Oxidoreductases Acting on Sulfur Group Donors/*genetics , Sulfur/*metabolism , Sulfur-Reducing Bacteria/*enzymology, Actinobacteria/genetics ; Bacterial Proteins/classification ; Genetic Variation ; Geologic Sediments/microbiology ; Oxidoreductases Acting on Sulfur Group Donors/classification ; Phylogeny ; Polymerase Chain Reaction ; Rhodococcus/enzymology ; Rhodococcus/genetics ; Soil Microbiology ; Sulfur-Reducing Bacteria/genetics ; Thiophenes/metabolism |
| مستخلص: | COnsensus DEgenerate Hybrid Oligonucleotide Primers (CODEHOP) were developed for the detection of the dszB desulfinase gene (2'-hydroxybiphenyl-2-sulfinate desulfinase; EC 3.13.1.3) by polymerase chain reaction (PCR), which allow to reveal larger diversity than traditional primers. The new developed primers were used as molecular monitoring tool to drive a procedure for the isolation of desulfurizing microorganisms. The primers revealed a large dszB gene diversity in environmental samples, particularly in diesel-contaminated soil that served as inoculum for enrichment cultures. The isolation procedure using the dibenzothiophene sulfone (DBTO Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest. (Copyright © 2021 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 16S rRNA gene; Desulfurization; Dibenzothiophene sulfone; Gordonia; dszB desulfinase gene |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Thiophenes) 70FD1KFU70 (Sulfur) EC 1.8.- (2-(2-hydroxybiphenyl)-benzenesulfinate desulfinase) EC 1.8.- (Oxidoreductases Acting on Sulfur Group Donors) Z3D4AJ1R48 (dibenzothiophene) |
| SCR Organism: | Rhodococcus erythropolis |
| تواريخ الأحداث: | Date Created: 20210810 Date Completed: 20211228 Latest Revision: 20220714 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.resmic.2021.103872 |
| PMID: | 34375709 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1769-7123 |
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| DOI: | 10.1016/j.resmic.2021.103872 |