دورية أكاديمية
أعرض في EDS

Proteomics informed by transcriptomics for a qualitative and quantitative analysis of the sialoproteome of adult Ornithodoros moubata ticks.

التفاصيل البيبلوغرافية
العنوان: Proteomics informed by transcriptomics for a qualitative and quantitative analysis of the sialoproteome of adult Ornithodoros moubata ticks.
المؤلفون: Oleaga A; Parasitology Laboratory, Institute of Natural Resources and Agrobiology (IRNASA, CSIC), Salamanca, Spain. ana.oleaga@irnasa.csic.es., Carnero-Morán A; Parasitology Laboratory, Institute of Natural Resources and Agrobiology (IRNASA, CSIC), Salamanca, Spain., Valero ML; Proteomics Section, Central Service for Experimental Research, University of Valencia, Valencia, Spain., Pérez-Sánchez R; Parasitology Laboratory, Institute of Natural Resources and Agrobiology (IRNASA, CSIC), Salamanca, Spain.
المصدر: Parasites & vectors [Parasit Vectors] 2021 Aug 11; Vol. 14 (1), pp. 396. Date of Electronic Publication: 2021 Aug 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central
مواضيع طبية MeSH: Gene Expression Profiling* , Proteome* , Proteomics*, Ornithodoros/*genetics , Saliva/*chemistry , Salivary Proteins and Peptides/*analysis, Animals ; Arthropod Proteins ; Chromatography, Liquid ; Female ; Male ; Ornithodoros/chemistry ; Reproducibility of Results ; Tandem Mass Spectrometry
مستخلص: Background: The argasid tick Ornithodoros moubata is the main vector in mainland Africa of African swine fever virus and the spirochete Borrelia duttoni, which causes human relapsing fever. The elimination of populations of O. moubata would contribute to the prevention and control of these two serious diseases. Anti-tick vaccines are an eco-friendly and sustainable means of eliminating tick populations. Tick saliva forms part of the tick-host interface, and knowledge of its composition is key to the identification and selection of vaccine candidate antigens. The aim of the present work is to increase the body of data on the composition of the saliva proteome of adult O. moubata ticks, particularly of females, since in-depth knowledge of the O. moubata sialome will allow the identification and selection of novel salivary antigens as targets for tick vaccines.
Methods: We analysed samples of female and male saliva using two different mass spectrometry (MS) approaches: data-dependent acquisition liquid chromatography-tandem MS (LC-MS/MS) and sequential window acquisition of all theoretical fragment ion spectra-MS (SWATH-MS). To maximise the number of proteins identified, a proteomics informed by transcriptomics analysis was applied using the O. moubata salivary transcriptomic dataset previously obtained by RNA-Seq.
Results: SWATH-MS proved to be superior to LC-MS/MS for the study of female saliva, since it identified 61.2% more proteins than the latter, the reproducibility of results was enhanced with its use, and it provided a quantitative picture of salivary components. In total, we identified 299 non-redundant proteins in the saliva of O. moubata, and quantified the expression of 165 of these in both male and female saliva, among which 13 were significantly overexpressed in females and 40 in males. These results indicate important quantitative differences in the saliva proteome between the sexes.
Conclusions: This work expands our knowledge of the O. moubata sialome, particularly that of females, by increasing the number of identified novel salivary proteins, which have different functions at the tick-host feeding interface. This new knowledge taken together with information on the O. moubata sialotranscriptome will allow a more rational selection of salivary candidates as antigen targets for tick vaccine development.
(© 2021. The Author(s).)
References: Int J Parasitol. 2014 May;44(6):369-79. (PMID: 24583183)
J Proteomics. 2013 Mar 27;80:216-35. (PMID: 23416086)
Nat Commun. 2017 Aug 21;8(1):291. (PMID: 28827567)
Vet Parasitol. 2017 Aug 30;243:119-124. (PMID: 28807279)
Parasite. 2009 Sep;16(3):191-202. (PMID: 19839264)
PLoS Negl Trop Dis. 2020 Feb 12;14(2):e0007758. (PMID: 32049966)
Peptides. 2006 Apr;27(4):667-74. (PMID: 16191451)
Ticks Tick Borne Dis. 2017 Aug;8(5):693-708. (PMID: 28528879)
Insect Biochem Mol Biol. 2007 Nov;37(11):1149-59. (PMID: 17916501)
J Proteome Res. 2012 Dec 7;11(12):5972-82. (PMID: 23140423)
Vet Parasitol. 2015 Sep 15;212(3-4):336-49. (PMID: 26293586)
Sci Rep. 2019 May 1;9(1):6764. (PMID: 31043627)
Parasit Vectors. 2017 Aug 1;10(1):366. (PMID: 28764815)
Exp Appl Acarol. 2020 Mar;80(3):349-361. (PMID: 31927645)
Ticks Tick Borne Dis. 2015 Apr;6(3):211-20. (PMID: 25802033)
Mol Cell Proteomics. 2007 Sep;6(9):1638-55. (PMID: 17533153)
Parasite Immunol. 2021 May;43(5):e12807. (PMID: 33135186)
Ticks Tick Borne Dis. 2018 Jul;9(5):1158-1172. (PMID: 29728336)
Parasitology. 2004;129 Suppl:S3-14. (PMID: 15938502)
Nat Protoc. 2015 Mar;10(3):426-41. (PMID: 25675208)
Mass Spectrom Rev. 2014 Nov-Dec;33(6):452-70. (PMID: 24281846)
Vet Parasitol. 2013 Jan 31;191(3-4):301-14. (PMID: 23089148)
Blood. 2011 Jan 13;117(2):736-44. (PMID: 20940421)
J Parasitol. 1997 Oct;83(5):831-8. (PMID: 9379286)
J Proteomics. 2018 Oct 30;189:11-22. (PMID: 29501709)
Front Cell Infect Microbiol. 2017 Feb 07;7:23. (PMID: 28229048)
Front Cell Infect Microbiol. 2020 Sep 11;10:574405. (PMID: 33042874)
Ticks Tick Borne Dis. 2018 May;9(4):1006-1037. (PMID: 29625921)
Mol Syst Biol. 2018 Aug 13;14(8):e8126. (PMID: 30104418)
Transbound Emerg Dis. 2016 Aug;63(4):443-51. (PMID: 25483914)
Virus Res. 2013 Apr;173(1):228-46. (PMID: 23142552)
Insect Mol Biol. 2010 Aug;19(4):501-15. (PMID: 20456507)
Vet Parasitol. 2011 May 31;178(1-2):163-72. (PMID: 21216104)
Front Cell Infect Microbiol. 2018 Apr 04;8:98. (PMID: 29670860)
J Med Microbiol. 2020 Jun;69(6):781-791. (PMID: 32478654)
Insect Biochem Mol Biol. 2008 Sep;38(9):841-52. (PMID: 18694828)
J Appl Microbiol. 2010 Apr;108(4):1115-22. (PMID: 19886891)
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450. (PMID: 30395289)
Front Biosci (Landmark Ed). 2009 Jan 01;14:2051-88. (PMID: 19273185)
Parasit Vectors. 2021 Mar 20;14(1):170. (PMID: 33743776)
J Biol Chem. 2008 Jul 4;283(27):18721-33. (PMID: 18445596)
PLoS Negl Trop Dis. 2021 Feb 5;15(2):e0009105. (PMID: 33544727)
Trends Parasitol. 2016 Mar;32(3):242-254. (PMID: 26520005)
Transbound Emerg Dis. 2019 Mar;66(2):672-686. (PMID: 30633851)
Transbound Emerg Dis. 2011 Jun;58(3):247-54. (PMID: 21320295)
BMC Genomics. 2019 Aug 27;20(1):675. (PMID: 31455241)
J Proteomics. 2015 Mar 18;117:120-44. (PMID: 25576852)
Proteomics. 2010 Jun;10(11):2089-98. (PMID: 20340161)
Int J Parasitol. 2012 May 1;42(5):421-7. (PMID: 22549026)
Vet Rec. 1990 Jan 13;126(2):32-7. (PMID: 2301109)
Ticks Tick Borne Dis. 2018 Jul;9(5):1354-1357. (PMID: 29656834)
معلومات مُعتمدة: RTI2018-098297-B-I00 Ministerio de Ciencia, Innovación y Universidades; CLU-2019-05 junta de castilla y león and the european union
فهرسة مساهمة: Keywords: LC-MS/MS; Ornithodoros moubata; Proteome; SWATH-MS; Saliva
المشرفين على المادة: 0 (Arthropod Proteins)
0 (Proteome)
0 (Salivary Proteins and Peptides)
تواريخ الأحداث: Date Created: 20210812 Date Completed: 20211119 Latest Revision: 20211119
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8356541
DOI: 10.1186/s13071-021-04892-2
PMID: 34380568
قاعدة البيانات: MEDLINE
الوصف
تدمد:1756-3305
DOI:10.1186/s13071-021-04892-2