دورية أكاديمية
Protocols for endothelial cell isolation from mouse tissues: kidney, spleen, and testis.
| العنوان: | Protocols for endothelial cell isolation from mouse tissues: kidney, spleen, and testis. |
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| المؤلفون: | Dumas SJ; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Meta E; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Conchinha NV; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Sokol L; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Chen R; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong, P.R. China., Borri M; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Teuwen LA; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.; Translational Cancer Research Unit, GZA Hospitals Sint-Augustinus, Antwerp 2610, Belgium.; Center for Oncological Research, University of Antwerp, Antwerp 2000, Belgium., Veys K; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., García-Caballero M; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Geldhof V; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Treps L; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., de Zeeuw P; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Falkenberg KD; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Dubois C; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Parys M; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., de Rooij LPMH; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Rohlenova K; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Goveia J; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Schoonjans L; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong, P.R. China., Dewerchin M; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Eelen G; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium., Li X; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong, P.R. China., Kalucka J; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.; Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark., Carmeliet P; Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong, P.R. China.; Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark. |
| المصدر: | STAR protocols [STAR Protoc] 2021 Jul 28; Vol. 2 (3), pp. 100523. Date of Electronic Publication: 2021 Jul 28 (Print Publication: 2021). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: eCollection Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Endothelial Cells/*cytology , Kidney/*cytology , Spleen/*cytology , Testis/*cytology, Animals ; Flow Cytometry ; Male ; Mice |
| مستخلص: | Endothelial cells (ECs) exhibit phenotypic and functional tissue specificities, critical for studies in the vascular field and beyond. Thus, tissue-specific methods for isolation of highly purified ECs are necessary. Kidney, spleen, and testis ECs are relevant players in health and diseases such as chronic kidney disease, acute kidney injury, myelofibrosis, and cancer. Here, we provide tailored protocols for rapid and reproducible EC purification established for scRNA sequencing from these adult murine tissues using the combination of magnetic- and fluorescence-activated cell sorting. For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020) and Dumas et al. (2020). Competing Interests: The authors declare no competing interests. (© 2021 The Author(s).) |
| References: | Cell. 2020 Feb 20;180(4):764-779.e20. (PMID: 32059779) Bioinformatics. 2017 Sep 01;33(17):2776-2778. (PMID: 28475694) Bio Protoc. 2018 Aug 05;8(15):e2962. (PMID: 34395767) Nucleic Acids Res. 2020 Jul 2;48(W1):W385-W394. (PMID: 32392297) PLoS Biol. 2020 Apr 6;18(4):e3000704. (PMID: 32251437) J Am Soc Nephrol. 2020 Jan;31(1):118-138. (PMID: 31818909) Nat Biotechnol. 2015 May;33(5):495-502. (PMID: 25867923) |
| فهرسة مساهمة: | Keywords: Cell isolation; Flow Cytometry/Mass Cytometry; Single Cell |
| تواريخ الأحداث: | Date Created: 20210812 Date Completed: 20220322 Latest Revision: 20240403 |
| رمز التحديث: | 20240403 |
| مُعرف محوري في PubMed: | PMC8339245 |
| DOI: | 10.1016/j.xpro.2021.100523 |
| PMID: | 34382011 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2021.100523 |