دورية أكاديمية
Proteome and functional decline as platelets age in the circulation.
| العنوان: | Proteome and functional decline as platelets age in the circulation. |
|---|---|
| المؤلفون: | Allan HE; Centre for Immunobiology, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK., Hayman MA; Centre for Immunobiology, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK., Marcone S; Department of Surgery, Trinity Translational Medicine Institute, Trinity College Dublin, Dublin, Ireland., Chan MV; Centre for Immunobiology, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK., Edin ML; National Institutes of Health, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA., Maffucci T; Centre for Cell Biology and Cutaneous Research, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK., Joshi A; King's British Heart Foundation Centre, Kings College London, London, UK., Menke L; Centre for Immunobiology, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK., Crescente M; Centre for Immunobiology, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK., Mayr M; King's British Heart Foundation Centre, Kings College London, London, UK., Zeldin DC; National Institutes of Health, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA., Armstrong PC; Centre for Immunobiology, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK., Warner TD; Centre for Immunobiology, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK. |
| المصدر: | Journal of thrombosis and haemostasis : JTH [J Thromb Haemost] 2021 Dec; Vol. 19 (12), pp. 3095-3112. Date of Electronic Publication: 2021 Aug 27. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 101170508 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1538-7836 (Electronic) Linking ISSN: 15387836 NLM ISO Abbreviation: J Thromb Haemost Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [New York] : Elsevier Original Publication: Oxford : Blackwell Pub. |
| مواضيع طبية MeSH: | Proteome* , Thrombocytopenia*, Blood Platelets ; Hemostasis ; Humans ; Proteomics |
| مستخلص: | Background: Platelets circulate in the blood of healthy individuals for approximately 7-10 days regulated by finely balanced processes of production and destruction. As platelets are anucleate we reasoned that their protein composition would change as they age and that this change would be linked to alterations in structure and function. Objective: To isolate platelets of different ages from healthy individuals to test the hypothesis that changes in protein content cause alterations in platelet structure and function. Methods: Platelets were separated according to thiazole orange fluorescence intensity as a surrogate indicator of mRNA content and so a marker of platelet age and then subjected to proteomics, imaging, and functional assays to produce an in-depth analysis of platelet composition and function. Results: Total protein content was 45 ± 5% lower in old platelets compared to young platelets. Predictive proteomic pathway analysis identified associations with 28 biological processes, notably higher hemostasis in young platelets whilst apoptosis and senescence were higher in old platelets. Further studies confirmed platelet ageing was linked to a decrease in cytoskeletal protein and associated capability to spread and adhere, a reduction in mitochondria number, and lower calcium dynamics and granule secretion. Conclusions: Our findings demonstrate changes in protein content are linked to alterations in function as platelets age. This work delineates physical and functional changes in platelets as they age and serves as a base to examine differences associated with altered mean age of platelet populations in conditions such as immune thrombocytopenia and diabetes. (© 2021 The Authors. Journal of Thrombosis and Haemostasis published by Wiley Periodicals LLC on behalf of International Society on Thrombosis and Haemostasis.) |
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| معلومات مُعتمدة: | 101604/Z/13/Z United Kingdom WT_ Wellcome Trust; Z01 ES025034 United States ImNIH Intramural NIH HHS; RG/19/8/34500 United Kingdom BHF_ British Heart Foundation; RG/16/14/32397 United Kingdom BHF_ British Heart Foundation; PG/17/40/33028 United Kingdom BHF_ British Heart Foundation; CH/16/3/32406 United Kingdom BHF_ British Heart Foundation; PG/15/47/31591 United Kingdom BHF_ British Heart Foundation; FS/16/32/32184 United Kingdom BHF_ British Heart Foundation; United Kingdom WT_ Wellcome Trust |
| فهرسة مساهمة: | Keywords: ageing; blood platelets; cytoskeleton; mitochondria; proteomics |
| المشرفين على المادة: | 0 (Proteome) |
| تواريخ الأحداث: | Date Created: 20210814 Date Completed: 20211208 Latest Revision: 20230829 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC8604765 |
| DOI: | 10.1111/jth.15496 |
| PMID: | 34390534 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1538-7836 |
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| DOI: | 10.1111/jth.15496 |