دورية أكاديمية
Chemical constituents and anti-inflammatory activity of the total alkaloid extract from Melodinus cochinchinensis (Lour.) Merr. and its inhibition of the NF-κB and MAPK signaling pathways.
| العنوان: | Chemical constituents and anti-inflammatory activity of the total alkaloid extract from Melodinus cochinchinensis (Lour.) Merr. and its inhibition of the NF-κB and MAPK signaling pathways. |
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| المؤلفون: | Yang M; Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, China., Wang Y; Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, China., Fan Z; Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, China., Xue Q; Department of Chemistry, Liaocheng University, Liaocheng 252059 Shandong, China., Njateng GSS; Department of Biochemistry, Faculty of science, University of Dschang, P.O. Box 67, Dschang, Cameroon., Liu Y; Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, China., Cao J; Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, China., Khan A; Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, Pakistan., Cheng G; Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, China. Electronic address: ggcheng@kust.edu.cn. |
| المصدر: | Phytomedicine : international journal of phytotherapy and phytopharmacology [Phytomedicine] 2021 Oct; Vol. 91, pp. 153684. Date of Electronic Publication: 2021 Jul 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Urban & Fischer Verlag Country of Publication: Germany NLM ID: 9438794 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-095X (Electronic) Linking ISSN: 09447113 NLM ISO Abbreviation: Phytomedicine Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Stuttgart : Urban & Fischer Verlag Original Publication: Stuttgart ; New York : G. Fischer, c1994- |
| مواضيع طبية MeSH: | Alkaloids*/pharmacology , Alkaloids*/therapeutic use , Anti-Inflammatory Agents*/pharmacology , Anti-Inflammatory Agents*/therapeutic use , Edema*/drug therapy , Plant Extracts*/pharmacology , Plant Extracts*/therapeutic use, Apocynaceae/*chemistry, Animals ; Cytokines/metabolism ; Lipopolysaccharides ; MAP Kinase Signaling System/drug effects ; Mice ; NF-kappa B/metabolism ; RAW 264.7 Cells |
| مستخلص: | Background: Melodinus cochinchinensis (Lour.) Merr. is a medicinal plant, which is used as a folk medicine for treating meningitis and fractures. However, the anti-inflammatory activity of total alkaloid extract from M. cochinchinensis (MCTA) and its molecular mechanism are still not studied. Purpose: The aim of this study is to investigate the main chemical constituents of MCTA and explore its anti-inflammatory potential in both in vitro and in vivo assessments. Methods: UHPLC-ESI-HRMS/MS was applied to analyze the chemical profiling. The anti-inflammatory efficacy of MCTA was evaluated on lipopolysaccharide (LPS) induced RAW 264.7 cells and two common inflammation models in mice. The production of pro-inflammatory mediator and cytokine was tested using the ELISA method. The pathological change was analyzed by histological assessment. The expression of NF-κB, MAPKs and PPAR-γ proteins was evaluated using western blot analysis. Results: A total of 21 monoterpenoid indole alkaloids (MIAs) were characterized by UHPLC-ESI-HRMS/MS. Aspidospermine- and quinolone-type alkaloids were found to be the major compounds. MCTA significantly decreased the production of NO, IL-1β, IL-6 and TNF-α in LPS-induced RAW 264.7 macrophages. MCTA significantly inhibited the phosphorylation of ERK1/2, JNK and p38 MAPK, suppressed the NF-κB transcriptional activation and improved the PPAR-γ expression. Moreover, the in vivo experiment exhibited that MCTA pretreatment markedly alleviated the xylene-induced ear edema and carrageenan-induced paw edema in mice and decreased the IL-1β, IL-6 and TNF-α expressions. Conclusion: MCTA is rich in MIAs and exhibited a significant inhibitory effect on the production proinflammatory cytokines. The mechanism might be related to the inhibition of activation of NF-κB and MAPK pathways. (Copyright © 2021. Published by Elsevier GmbH.) |
| فهرسة مساهمة: | Keywords: Anti-inflammatory activity; Ear edema; Melodinus cochinchinensis (Lour.) Merr.; Monoterpenoid indole alkaloids; Paw edema; Signaling pathway |
| المشرفين على المادة: | 0 (Alkaloids) 0 (Anti-Inflammatory Agents) 0 (Cytokines) 0 (Lipopolysaccharides) 0 (NF-kappa B) 0 (Plant Extracts) |
| تواريخ الأحداث: | Date Created: 20210817 Date Completed: 20211006 Latest Revision: 20211006 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.phymed.2021.153684 |
| PMID: | 34400050 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1618-095X |
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| DOI: | 10.1016/j.phymed.2021.153684 |