دورية أكاديمية
أعرض في EDS

Intranasal vaccine from whole Leishmania donovani antigens provides protection and induces specific immune response against visceral leishmaniasis.

التفاصيل البيبلوغرافية
العنوان: Intranasal vaccine from whole Leishmania donovani antigens provides protection and induces specific immune response against visceral leishmaniasis.
المؤلفون: Helou DG; Université Paris-Saclay, CNRS, BioCis-UMR 8076, Châtenay-Malabry, France., Mauras A; Université Paris-Saclay, CNRS, BioCis-UMR 8076, Châtenay-Malabry, France., Fasquelle F; Université de Lille, Inserm, LIRIC-UMR 995, Lille, France., Lanza JS; Université Paris-Saclay, CNRS, BioCis-UMR 8076, Châtenay-Malabry, France., Loiseau PM; Université Paris-Saclay, CNRS, BioCis-UMR 8076, Châtenay-Malabry, France., Betbeder D; Université de Lille, Inserm, LIRIC-UMR 995, Lille, France., Cojean S; Université Paris-Saclay, CNRS, BioCis-UMR 8076, Châtenay-Malabry, France.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2021 Aug 17; Vol. 15 (8), pp. e0009627. Date of Electronic Publication: 2021 Aug 17 (Print Publication: 2021).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Antigens, Protozoan/*immunology , Bone Marrow/*parasitology , Leishmaniasis Vaccines/*immunology , Leishmaniasis, Visceral/*immunology , Liver/*parasitology , Spleen/*parasitology, Adaptive Immunity ; Adjuvants, Immunologic/administration & dosage ; Administration, Intranasal ; Animals ; Antibodies, Protozoan/blood ; Antigens, Protozoan/administration & dosage ; Antigens, Protozoan/blood ; Bone Marrow/metabolism ; Female ; Immunization ; Interferon-gamma/metabolism ; Leishmania donovani/immunology ; Leishmaniasis Vaccines/administration & dosage ; Leishmaniasis, Visceral/parasitology ; Leishmaniasis, Visceral/prevention & control ; Liver/metabolism ; Mice ; Mice, Inbred BALB C ; Spleen/metabolism
مستخلص: Visceral leishmaniasis is a protozoan disease associated with high fatality rate in developing countries. Although the drug pipeline is constantly improving, available treatments are costly and live-threatening side effects are not uncommon. Moreover, an approved vaccine against human leishmaniasis does not exist yet. Using whole antigens from Leishmania donovani promastigotes (LdAg), we investigated the protective potential of a novel adjuvant-free vaccine strategy. Immunization of mice with LdAg via the intradermal or the intranasal route prior to infection decreases the parasitic burden in primary affected internal organs, including the liver, spleen, and bone marrow. Interestingly, the intranasal route is more efficient than the intradermal route, leading to better parasite clearance and remarkable induction of adaptive immune cells, notably the helper and cytotoxic T cells. In vitro restimulation experiments with Leishmania antigens led to significant IFN-γ secretion by splenocytes; therefore, exemplifying specificity of the adaptive immune response. To improve mucosal delivery and the immunogenic aspects of our vaccine strategy, we used polysaccharide-based nanoparticles (NP) that carry the antigens. The NP-LdAg formulation is remarkably taken up by dendritic cells and induces their maturation in vitro, as revealed by the increased expression of CD80, CD86 and MHC II. Intranasal immunization with NP-LdAg does not improve the parasite clearance in our experimental timeline; however, it does increase the percentage of effector and memory T helper cells in the spleen, suggesting a potential induction of long-term memory. Altogether, this study provides a simple and cost-effective vaccine strategy against visceral leishmaniasis based on LdAg administration via the intranasal route, which could be applicable to other parasitic diseases.
Competing Interests: The authors have declared that no competing interests exist.
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المشرفين على المادة: 0 (Adjuvants, Immunologic)
0 (Antibodies, Protozoan)
0 (Antigens, Protozoan)
0 (Leishmaniasis Vaccines)
82115-62-6 (Interferon-gamma)
تواريخ الأحداث: Date Created: 20210817 Date Completed: 20211116 Latest Revision: 20211116
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8370633
DOI: 10.1371/journal.pntd.0009627
PMID: 34403413
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0009627