دورية أكاديمية
Increased CG, CHG and CHH methylation at the cycloidea gene in the "Peloria" mutant of Linaria vulgaris.
| العنوان: | Increased CG, CHG and CHH methylation at the cycloidea gene in the "Peloria" mutant of Linaria vulgaris. |
|---|---|
| المؤلفون: | Ehrenhofer-Murray AE; Institut für Biologie, Lebenswissenschaftliche Fakultät, Humboldt-Universität zu Berlin, 10099, Berlin, Germany. Electronic address: ann.ehrenhofer-murray@hu-berlin.de. |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2021 Oct 08; Vol. 573, pp. 112-116. Date of Electronic Publication: 2021 Aug 08. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | DNA, Plant/*genetics , Linaria/*genetics, DNA Methylation ; Gene Expression Regulation, Plant/genetics ; Mutation |
| مستخلص: | Heritable DNA methylation variation is frequently observed in natural populations of plants, but is thought mostly to be functionally inconsequential. An exception to this is the "Peloria" mutant of Linaria vulgaris, which was originally described by Carl von Linné in 1744. A study in 1999 found that the Peloria phenotype is caused by an epiallele of the L. vulgaris cycloidea homolog Lcyc that showed increased levels of DNA methylation compared to wild-type. The DNA methylation results in silencing of Lcyc, which causes radial flower symmetry in the peloric mutant, whereas wild-type plants have flowers with bilateral symmetry. However, a detailed view of DNA methylation at Lcyc at the single-nucleotide level has not been available. In this study, we investigated DNA methylation at Lcyc and, as a control, at the LvHIRZ gene in wild-type and peloric plants of L. vulgaris using DNA bisulfite treatment coupled to next-generation sequencing. We found strong increases in CHG and CHH methylation at Lcyc, but not LvHIRZ, in Peloria. CG methylation was also increased, but wild-type Lcyc also showed moderate levels of CG methylation. Our results suggest that DNA methylation in all three sequence contexts has been maintained, and potentially transgenerationally inherited, in the peloric L. vulgaris population over decades or even centuries. Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Ann Ehrenhofer-Murray reports article publishing charges and equipment, drugs, or supplies were provided by Humboldt University of Berlin. Ann Ehrenhofer-Murray reports a relationship with Humboldt University of Berlin that includes: employment. (Copyright © 2021 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Linaria vulgaris; LvHIRZ; Peloria; cycloidea; peloric |
| المشرفين على المادة: | 0 (DNA, Plant) |
| تواريخ الأحداث: | Date Created: 20210817 Date Completed: 20211118 Latest Revision: 20211118 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.bbrc.2021.08.007 |
| PMID: | 34403807 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2104 |
|---|---|
| DOI: | 10.1016/j.bbrc.2021.08.007 |