دورية أكاديمية
أعرض في EDS

Impaired brown adipose tissue is differentially modulated in insulin-resistant obese wistar and type 2 diabetic Goto-Kakizaki rats.

التفاصيل البيبلوغرافية
العنوان: Impaired brown adipose tissue is differentially modulated in insulin-resistant obese wistar and type 2 diabetic Goto-Kakizaki rats.
المؤلفون: Serdan TDA; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil. Electronic address: tamiresd.serdan@gmail.com., Masi LN; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil., Pereira JNB; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil., Rodrigues LE; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil., Alecrim AL; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil., Scervino MVM; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil., Diniz VLS; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil., Dos Santos AAC; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil., Filho CPBS; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil., Alba-Loureiro TC; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil., Marzuca-Nassr GN; Department of Internal Medicine, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile., Bazotte RB; Department of Pharmacology and Therapeutics, State University of Maringa, Paraná, Brazil., Gorjão R; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil., Pithon-Curi TC; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil., Curi R; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil., Hirabara SM; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, Brazil.
المصدر: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2021 Oct; Vol. 142, pp. 112019. Date of Electronic Publication: 2021 Aug 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Editions Scientifiques Elsevier Country of Publication: France NLM ID: 8213295 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1950-6007 (Electronic) Linking ISSN: 07533322 NLM ISO Abbreviation: Biomed Pharmacother Subsets: MEDLINE
أسماء مطبوعة: Publication: Paris : Editions Scientifiques Elsevier
Original Publication: New York, N.Y. : Masson Pub. USA, Inc., c1982-
مواضيع طبية MeSH: Adipose Tissue, Brown/*metabolism , Diabetes Mellitus, Type 2/*metabolism , Insulin Resistance/*physiology , Obesity/*metabolism, Animals ; Diabetes Mellitus, Experimental/metabolism ; Diet, High-Fat ; Fluorodeoxyglucose F18 ; Glucose/metabolism ; Male ; Positron-Emission Tomography ; Rats ; Rats, Wistar
مستخلص: Brown adipose tissue (BAT) is a potential target to treat obesity and diabetes, dissipating energy as heat. Type 2 diabetes (T2D) has been associated with obesogenic diets; however, T2D was also reported in lean individuals to be associated with genetic factors. We aimed to investigate the differences between obese and lean models of insulin resistance (IR) and elucidate the mechanism associated with BAT metabolism and dysfunction in different IR animal models: a genetic model (lean GK rats) and obese models (diet-induced obese Wistar rats) at 8 weeks of age fed a high-carbohydrate (HC), high-fat (HF) diet, or high-fat and high-sugar (HFHS) diet for 8 weeks. At 15 weeks of age, BAT glucose uptake was evaluated by 18F-FDG PET under basal (saline administration) or stimulated condition (CL316,243, a selective β3-AR agonist). After CL316, 243 administrations, GK animals showed decreased glucose uptake compared to HC animals. At 16 weeks of age, the animals were euthanized, and the interscapular BAT was dissected for analysis. Histological analyses showed lower cell density in GK rats and higher adipocyte area compared to all groups, followed by HFHS and HF compared to HC. HFHS showed a decreased batokine FGF21 protein level compared to all groups. However, GK animals showed increased expression of genes involved in fatty acid oxidation (CPT1 and CPT2), BAT metabolism (Sirt1 and Pgc1-α), and obesogenic genes (leptin and PAI-1) but decreased gene expression of glucose transporter 1 (GLUT-1) compared to other groups. Our data suggest impaired BAT function in obese Wistar and GK rats, with evidence of a whitening process in these animals.
(Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
التعليقات: Erratum in: Biomed Pharmacother. 2022 Feb;146:112612. (PMID: 34998644)
فهرسة مساهمة: Keywords: Batokines; Glucose uptake; Obesity; Thermogenesis; Type 2 diabetes
المشرفين على المادة: 0Z5B2CJX4D (Fluorodeoxyglucose F18)
IY9XDZ35W2 (Glucose)
تواريخ الأحداث: Date Created: 20210817 Date Completed: 20211223 Latest Revision: 20220109
رمز التحديث: 20231215
DOI: 10.1016/j.biopha.2021.112019
PMID: 34403962
قاعدة البيانات: MEDLINE
الوصف
تدمد:1950-6007
DOI:10.1016/j.biopha.2021.112019