دورية أكاديمية
أعرض في EDS

Mutual regulation between chicken telomerase reverse transcriptase and the Wnt/β-catenin signalling pathway inhibits apoptosis and promotes the replication of ALV-J in LMH cells.

التفاصيل البيبلوغرافية
العنوان: Mutual regulation between chicken telomerase reverse transcriptase and the Wnt/β-catenin signalling pathway inhibits apoptosis and promotes the replication of ALV-J in LMH cells.
المؤلفون: Xiang Y; College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China., Yu Y; College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China., Li Q; College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China., Jiang Z; College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China., Li J; College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China., Liang C; College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China., Chen J; College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China., Li Y; College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China., Chen X; College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China., Cao W; College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China. caoscauvet@163.com.; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, 510642, China. caoscauvet@163.com.; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. caoscauvet@163.com.; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, South China Agricultural University, Guangzhou, 510642, China. caoscauvet@163.com.; Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, Guangzhou, 510642, China. caoscauvet@163.com.
المصدر: Veterinary research [Vet Res] 2021 Aug 19; Vol. 52 (1), pp. 110. Date of Electronic Publication: 2021 Aug 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 9309551 Publication Model: Electronic Cited Medium: Internet ISSN: 1297-9716 (Electronic) Linking ISSN: 09284249 NLM ISO Abbreviation: Vet Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 2011- : London : BioMed Central
Original Publication: Paris : Editions Scientifiques Elsevier ; INRA, c1993-
مواضيع طبية MeSH: Cell Movement* , Virus Replication* , Wnt Signaling Pathway*, Apoptosis/*genetics , Avian Leukosis Virus/*physiology , Avian Proteins/*genetics , Chickens/*physiology , Telomerase/*genetics, Animals ; Avian Leukosis/pathology ; Avian Proteins/metabolism ; Carcinogenesis ; Cell Line ; Chickens/genetics ; Poultry Diseases/pathology ; Telomerase/metabolism
مستخلص: This study aimed to explore the mutual regulation between chicken telomerase reverse transcriptase (chTERT) and the Wnt/β-catenin signalling pathway and its effects on cell growth and avian leukosis virus subgroup J (ALV-J) replication in LMH cells. First, LMH cells stably overexpressing the chTERT gene (LMH-chTERT cells) and corresponding control cells (LMH-NC cells) were successfully constructed with a lentiviral vector expression system. The results showed that chTERT upregulated the expression of β-catenin, Cyclin D1, TCF4 and c-Myc. chTERT expression level and telomerase activity were increased when cells were treated with LiCl. When the cells were treated with ICG001 or IWP-2, the activity of the Wnt/β-catenin signalling pathway was significantly inhibited, and chTERT expression and telomerase activity were also inhibited. However, when the β-catenin gene was knocked down by small interfering RNA (siRNA), the changes in chTERT expression and telomerase activity were consistent with those in cells treated with ICG001 or IWP-2. These results indicated that chTERT and the Wnt/β-catenin signalling pathway can be mutually regulated. Subsequently, we found that chTERT not only shortened the cell cycle to promote proliferation but also inhibited apoptosis by downregulating the expression of Caspase 3, Caspase 9 and BAX; upregulating BCL-2 and BCL-X expression; and promoting autophagy. Moreover, chTERT significantly enhanced the migration ability of LMH cells, upregulated the protein and mRNA expression of ALV-J and increased the virus titre. ALV-J replication promoted chTERT expression and telomerase activity.
(© 2021. The Author(s).)
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معلومات مُعتمدة: 2020B020222001 the Key-Area Research and Development Program of Guangdong Province; 2016YFD0501606 the National Key R&D Project; 2020KJ128 the Poultry Industry Technology System of Guangdong; CARS-41 china agriculture research system of mof and mara
فهرسة مساهمة: Keywords: Wnt/β-catenin signalling pathway; apoptosis; avian leukosis virus subgroup J; cell proliferation; chicken telomerase reverse transcriptase
المشرفين على المادة: 0 (Avian Proteins)
EC 2.7.7.49 (Telomerase)
تواريخ الأحداث: Date Created: 20210820 Date Completed: 20211101 Latest Revision: 20211101
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8375160
DOI: 10.1186/s13567-021-00979-x
PMID: 34412690
قاعدة البيانات: MEDLINE
الوصف
تدمد:1297-9716
DOI:10.1186/s13567-021-00979-x