دورية أكاديمية
أعرض في EDS

Accuracy of an experimental whole-blood test for detecting reactivation of echinococcal cysts.

التفاصيل البيبلوغرافية
العنوان: Accuracy of an experimental whole-blood test for detecting reactivation of echinococcal cysts.
المؤلفون: Petrone L; Translational Research Unit, National Institute for Infectious Diseases (INMI) 'Lazzaro Spallanzani'-IRCCS, Rome, Italy., Tamarozzi F; Department of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, Verona, Italy., Vola A; Microbiology and Virology Unit, Diagnostic Medicine Department, IRCCS San Matteo Hospital Foundation, Pavia, Italy., Gomez Morales MA; Foodborne and Neglected Parasitic Diseases Unit, Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy., Ludovisi A; Foodborne and Neglected Parasitic Diseases Unit, Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy., Najafi Fard S; Translational Research Unit, National Institute for Infectious Diseases (INMI) 'Lazzaro Spallanzani'-IRCCS, Rome, Italy., Mariconti M; Infectious Diseases and Immunology, IRCCS San Matteo Hospital Foundation, Pavia, Italy., Brunetti E; Infectious Diseases and Immunology, IRCCS San Matteo Hospital Foundation, Pavia, Italy.; Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy., Goletti D; Translational Research Unit, National Institute for Infectious Diseases (INMI) 'Lazzaro Spallanzani'-IRCCS, Rome, Italy.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2021 Aug 20; Vol. 15 (8), pp. e0009648. Date of Electronic Publication: 2021 Aug 20 (Print Publication: 2021).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Cysts/*immunology , Echinococcosis/*blood , Echinococcus granulosus/*immunology , Interleukin-4/*blood, Adult ; Aged ; Albendazole/therapeutic use ; Animals ; Cytokines/blood ; Echinococcosis/drug therapy ; Female ; Hematologic Tests ; Humans ; Life Cycle Stages/immunology ; Male ; Middle Aged ; Prospective Studies ; Treatment Outcome
مستخلص: Background: Cystic echinococcosis (CE) is a complex disease for which clear understanding of clinical manifestations is needed to avoid misdiagnosis, inappropriate treatment, and severe complications. We evaluated the accuracy of a whole-blood stimulation test based on Interleukin (IL)-4 detection in response to Antigen B (AgB) of Echinococcus granulosus sensu lato to discriminate cyst viability and detect cyst reactivation in patients with hepatic CE.
Methodology/principal Findings: Thirty patients with CE3b cysts and 37 patients with spontaneously-inactivated CE4-CE5 cysts were recruited (T0). After enrollment, 5 patients with CE3b cysts received albendazole, resulting in cyst solidification (CE4) in 4/5. Within a two-year follow-up, the whole-blood test was repeated at two time-points, in ≥14 (T1) and in ≥4 (T2) patients per group. IL-4 and a panel of other soluble factors were measured in the stimulated plasma. Baseline IL-4 levels were significantly higher in patients with CE3b compared to those with CE4 cysts (p = 0.006). Test accuracy for CE3b diagnosis had a sensitivity of 33-60% and a specificity of 76-95%, depending on the cut-off applied. Overall, IL-4 levels did not change significantly over time in either group; however, patients within the CE3b group showed a significant decrease of IL-1ra, IL-6, IL-8, G-CSF, IFN-γ, IP-10, MCP-1, MIP-1α, FGF at T1 compared to T0 (p≤0.042).
Conclusions/significance: Whole-blood IL-4-response to AgB is significantly higher in patients with active compared to inactive CE but apparently not modulated over time after treatment. On the contrary, the levels of IL-1ra, IL-6, IL-8, G-CSF, IFN-γ, IP-10, MCP-1, MIP-1α, FGF significantly decreased in active CE during follow-up. Additional studies are needed to understand whether these findings might have a clinical significance for patients' follow-up.
Competing Interests: The authors have declared that no competing interests exist.
References: Exp Parasitol. 2008 Aug;119(4):483-9. (PMID: 18329023)
Clin Vaccine Immunol. 2008 Jan;15(1):147-53. (PMID: 17989342)
Parasite Immunol. 1997 Dec;19(12):553-61. (PMID: 9458467)
PLoS Negl Trop Dis. 2015 Nov 17;9(11):e0004209. (PMID: 26575186)
PLoS Negl Trop Dis. 2018 Sep 6;12(9):e0006741. (PMID: 30188936)
Parasite Immunol. 2004 Jan;26(1):45-52. (PMID: 15198645)
PLoS Negl Trop Dis. 2009 Sep 29;3(9):e524. (PMID: 19787039)
Adv Parasitol. 2017;96:259-369. (PMID: 28212790)
Clin Exp Immunol. 1995 Mar;99(3):433-9. (PMID: 7882566)
Respirology. 2018 May;23(5):455-466. (PMID: 29457312)
Adv Parasitol. 2017;96:159-257. (PMID: 28212789)
Eur Respir J. 2020 Oct 1;56(4):. (PMID: 32586885)
Tuberculosis (Edinb). 2018 Jul;111:147-153. (PMID: 30029901)
Curr Opin Infect Dis. 2009 Oct;22(5):497-502. (PMID: 19633552)
Parasite Immunol. 2017 Dec;39(12):. (PMID: 29171068)
Clin Dev Immunol. 2012;2012:639362. (PMID: 22110535)
J Transl Med. 2015 May 13;13:157. (PMID: 25968473)
J Infect. 2015 Mar;70(3):299-306. (PMID: 25444973)
Parasite Immunol. 2010 Sep-Oct;32(9-10):696-700. (PMID: 20691021)
Biomed Res Int. 2015;2015:428205. (PMID: 26504805)
Parasite Immunol. 2020 Mar;42(3):e12695. (PMID: 31884696)
Clin Microbiol Infect. 2021 Feb;27(2):286.e7-286.e13. (PMID: 33045370)
BMC Infect Dis. 2014 Sep 09;14:492. (PMID: 25204575)
Acta Trop. 2010 Apr;114(1):1-16. (PMID: 19931502)
Am J Trop Med Hyg. 2016 Aug 3;95(2):405-9. (PMID: 27273641)
Am J Trop Med Hyg. 2018 Aug;99(2):375-379. (PMID: 29869600)
NMR Biomed. 2008 Aug;21(7):734-54. (PMID: 18384178)
Infect Immun. 2001 Jan;69(1):288-96. (PMID: 11119517)
Exp Parasitol. 2017 Jan;172:39-43. (PMID: 27988201)
Clin Dev Immunol. 2012;2012:483935. (PMID: 22400036)
Clin Infect Dis. 2020 Dec 3;71(9):2386-2388. (PMID: 32076699)
Parasitol Res. 2007 Apr;100(5):935-42. (PMID: 17111175)
J Interferon Cytokine Res. 1997 Apr;17(4):211-7. (PMID: 9142650)
PLoS Negl Trop Dis. 2014 Aug 14;8(8):e3057. (PMID: 25122222)
Emerg Infect Dis. 2006 Feb;12(2):296-303. (PMID: 16494758)
Parasit Vectors. 2020 Mar 14;13(1):133. (PMID: 32171321)
Eur Cytokine Netw. 2009 Jun;20(2):63-8. (PMID: 19541591)
PLoS Negl Trop Dis. 2020 Oct 5;14(10):e0008586. (PMID: 33017416)
Biomed Res Int. 2013;2013:978146. (PMID: 24151631)
Lancet Infect Dis. 2018 Jul;18(7):769-778. (PMID: 29793823)
المشرفين على المادة: 0 (Cytokines)
207137-56-2 (Interleukin-4)
F4216019LN (Albendazole)
تواريخ الأحداث: Date Created: 20210820 Date Completed: 20211117 Latest Revision: 20211117
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8378729
DOI: 10.1371/journal.pntd.0009648
PMID: 34415898
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0009648