دورية أكاديمية
Viability assessment of Mycobacterium tuberculosis complex in OMNIgene • SPUTUM reagent using the BACTEC MGIT 960 System and Xpert MTB/RIF assay.
العنوان: | Viability assessment of Mycobacterium tuberculosis complex in OMNIgene • SPUTUM reagent using the BACTEC MGIT 960 System and Xpert MTB/RIF assay. |
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المؤلفون: | Guirelli AO; Center of Regional Laboratory of Santo André, Instituto Adolfo Lutz, Santo André, SP, Brazil., Dos Santos Carmo AM; Center of Regional Laboratory of Santo André, Instituto Adolfo Lutz, Santo André, SP, Brazil., Eterovic A; Center for Natural and Human Sciences, Universidade Federal do ABC (UFABC), Santo André, SP, Brazil., Ferrazoli L; Center of Bacteriology, Department of Tuberculosis and Mycobacteriosis, Instituto Adolfo Lutz, São Paulo, SP, Brazil., Dos Santos Menezes Gaiotto Daros V; Center of Regional Laboratory of Santo André, Instituto Adolfo Lutz, Santo André, SP, Brazil., Cergole-Novella MC; Center of Regional Laboratory of Santo André, Instituto Adolfo Lutz, Santo André, SP, Brazil. maria.novella@ial.sp.gov.br. |
المصدر: | Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2021 Dec; Vol. 52 (4), pp. 1951-1957. Date of Electronic Publication: 2021 Aug 23. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Brazil NLM ID: 101095924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1678-4405 (Electronic) Linking ISSN: 15178382 NLM ISO Abbreviation: Braz J Microbiol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2019- : Switzerland, AG : Springer International Publishing Original Publication: Rio de Janeiro, RJ, Brasil : Sociedade Brasileira de Microbiologia |
مواضيع طبية MeSH: | Bacteriological Techniques*/instrumentation , Bacteriological Techniques*/methods , Microbial Viability* , Mycobacterium tuberculosis*/genetics , Sputum*/microbiology , Tuberculosis*/diagnosis , Tuberculosis*/microbiology, Humans ; Indicators and Reagents ; Sensitivity and Specificity |
مستخلص: | The World Health Organization advocates that sputum specimens submitted to tuberculosis (TB) diagnostic should be processed within 48 h after collection and be stored under cooling. We aimed to assess the performance of OMNIgene • SPUTUM reagent in maintaining viable specimens of Mycobacterium tuberculosis complex (MTBC) during transportation of sputum samples without refrigeration, in comparison to the standard protocol of the National TB Control Program. Sputum samples obtained in southeastern Brazil (June 2017 to July 2018) from 100 sequential patients with positive acid-fast bacillus smear microscopy were divided into two portions. Portion 1 continued to be cooled (standard protocol, STA), but portion 2 was added to OMNIgene • SPUTUM reagent (alternative protocol, OMS) until concomitant further processing. Both portions of all samples were cultured using MGIT and tested by Xpert MTB/RIF assay. Growth of MTBC in the first 42 days was detected in 96% of the cultures under the STA and 88% under the OMS. Intervals between processing and detecting MTBC growth in the two portions significantly differed (p = 0.0001). Portions under the two protocols showed similar results in the MTBC detection by Xpert assay and culture contamination by non-MTBC. The OMNIgene reagent liquefies and decontaminates sputum leading to a decrease in processing time. Although there was a small delay in mycobacterial growth, the OMNIgene reagent can be useful in specimens transported from collection sites over a long distance to centralized testing centers, maintaining viable MTBC for at least 8 days at room temperature. (© 2021. Sociedade Brasileira de Microbiologia.) |
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فهرسة مساهمة: | Keywords: BACTEC MGIT; OMNIgene • SPUTUM; Sputum transport; Tuberculosis; Xpert MTB/RIF |
المشرفين على المادة: | 0 (Indicators and Reagents) |
تواريخ الأحداث: | Date Created: 20210823 Date Completed: 20211215 Latest Revision: 20220824 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC8578262 |
DOI: | 10.1007/s42770-021-00568-2 |
PMID: | 34424510 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1678-4405 |
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DOI: | 10.1007/s42770-021-00568-2 |