دورية أكاديمية
Viability assessment of Mycobacterium tuberculosis complex in OMNIgene • SPUTUM reagent using the BACTEC MGIT 960 System and Xpert MTB/RIF assay.
| العنوان: | Viability assessment of Mycobacterium tuberculosis complex in OMNIgene • SPUTUM reagent using the BACTEC MGIT 960 System and Xpert MTB/RIF assay. |
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| المؤلفون: | Guirelli AO; Center of Regional Laboratory of Santo André, Instituto Adolfo Lutz, Santo André, SP, Brazil., Dos Santos Carmo AM; Center of Regional Laboratory of Santo André, Instituto Adolfo Lutz, Santo André, SP, Brazil., Eterovic A; Center for Natural and Human Sciences, Universidade Federal do ABC (UFABC), Santo André, SP, Brazil., Ferrazoli L; Center of Bacteriology, Department of Tuberculosis and Mycobacteriosis, Instituto Adolfo Lutz, São Paulo, SP, Brazil., Dos Santos Menezes Gaiotto Daros V; Center of Regional Laboratory of Santo André, Instituto Adolfo Lutz, Santo André, SP, Brazil., Cergole-Novella MC; Center of Regional Laboratory of Santo André, Instituto Adolfo Lutz, Santo André, SP, Brazil. maria.novella@ial.sp.gov.br. |
| المصدر: | Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2021 Dec; Vol. 52 (4), pp. 1951-1957. Date of Electronic Publication: 2021 Aug 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Brazil NLM ID: 101095924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1678-4405 (Electronic) Linking ISSN: 15178382 NLM ISO Abbreviation: Braz J Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Switzerland, AG : Springer International Publishing Original Publication: Rio de Janeiro, RJ, Brasil : Sociedade Brasileira de Microbiologia |
| مواضيع طبية MeSH: | Bacteriological Techniques*/instrumentation , Bacteriological Techniques*/methods , Microbial Viability* , Mycobacterium tuberculosis*/genetics , Sputum*/microbiology , Tuberculosis*/diagnosis , Tuberculosis*/microbiology, Humans ; Indicators and Reagents ; Sensitivity and Specificity |
| مستخلص: | The World Health Organization advocates that sputum specimens submitted to tuberculosis (TB) diagnostic should be processed within 48 h after collection and be stored under cooling. We aimed to assess the performance of OMNIgene • SPUTUM reagent in maintaining viable specimens of Mycobacterium tuberculosis complex (MTBC) during transportation of sputum samples without refrigeration, in comparison to the standard protocol of the National TB Control Program. Sputum samples obtained in southeastern Brazil (June 2017 to July 2018) from 100 sequential patients with positive acid-fast bacillus smear microscopy were divided into two portions. Portion 1 continued to be cooled (standard protocol, STA), but portion 2 was added to OMNIgene • SPUTUM reagent (alternative protocol, OMS) until concomitant further processing. Both portions of all samples were cultured using MGIT and tested by Xpert MTB/RIF assay. Growth of MTBC in the first 42 days was detected in 96% of the cultures under the STA and 88% under the OMS. Intervals between processing and detecting MTBC growth in the two portions significantly differed (p = 0.0001). Portions under the two protocols showed similar results in the MTBC detection by Xpert assay and culture contamination by non-MTBC. The OMNIgene reagent liquefies and decontaminates sputum leading to a decrease in processing time. Although there was a small delay in mycobacterial growth, the OMNIgene reagent can be useful in specimens transported from collection sites over a long distance to centralized testing centers, maintaining viable MTBC for at least 8 days at room temperature. (© 2021. Sociedade Brasileira de Microbiologia.) |
| References: | GLI-WHO (2014) Global Laboratory Initiative and Working Group of the Stop TB Partnership. Mycobacteriology Laboratory Manual, Genebra. http://www.stoptb.org/wg/gli/assets/documents/gli_mycobacteriology_lab_manual_web.pdf . Accessed February 2020. WHO-Technical Expert Group Meeting Report: (2017) Commercial products for preserving clinical specimens for the diagnosis of tuberculosis. Wada PY, Lee-Rodriguez C, Hung YY, Skarbinski J (2020) Burden of active tuberculosis in an integrated health care system, 1997-2016: incidence, mortality, and excess health care utilization. Open Forum Infect Dis 7(1):ofaa015. https://doi.org/10.1093/ofid/ofaa015. Maharjan B, Shrestha B, Weirich A, Stewart A, Kelly-Cirino CD (2016) A novel sputum transport solution eliminates cold chain and supports routine tuberculosis testing in Nepal. J Epidemiol Glob Health 6:257–265. https://doi.org/10.1016/j.jegh.2016.04.002. (PMID: 10.1016/j.jegh.2016.04.002271311037320459) Reeve BWP, McFall SM, Song R, Warren R, Steingart KR, Theron G (2018) Commercial products to preserve specimens for tuberculosis diagnosis: a systematic review. Int J Tuberc Lung Dis 22(7):741–753. https://doi.org/10.5588/ijtld.17.0816. (PMID: 10.5588/ijtld.17.081629914599) Ray B, Niles J, Stewart A, Kelly-Cirino C (2017) Optimizing high quality samples with OMNIgene®•SPUTUM for Mycobacterium tuberculosis culture. PD-WP-00043 Issue 3/2017-05. https://www.dnagenotek.com/US/pdf/PD-WP-00043.pdf . Accessed February 2020. DNA Genotek data sheet PD-BR-00196 Issue 1/2015-10. Available at: https://www.dnagenotek.com/US/pdf/PD-BR-00196.pdf . Accessed June 2017. Kelly-Cirino CD, Curry PS, Marola JL, Helstrom NK, Salfinger M (2016) Novel multi-day sputum transport reagent works with routine tuberculosis tests and eliminates need for cold chain: preliminary study of compatibility with the Xpert® MTBC/RIF assay. Diagn Microbiol Infect Dis 86:273–276. https://doi.org/10.1016/j.diagmicrobio.2016.08.013. (PMID: 10.1016/j.diagmicrobio.2016.08.01327601382) Kelly-Cirino CD, Musisi E, Byanyima P, Kaswabuli S, Andama A, Sessolo A (2017) Investigation of OMNIgene·SPUTUM performance in delayed tuberculosis testing by smear, culture, and Xpert MTB/RIF assays in Uganda. J Epidemiol Glob Health 7:103–109. https://doi.org/10.1016/j.jegh.2017.04.001. Ardizzoni E, Orikiriza P, Ssuuna C, Nyehangane D, Gumsboga M, Taremwa IM, Turyashemererwa E, Mwanga-Amumpaire J, Langendorf C, Bonnet M (2019) Evaluation of OMNIgene • SPUTUM and ethanol reagent for preservation of sputum prior to Xpert and culture testing in Uganda. J Clin Microbiol 58(1):e00810–e00819. https://doi.org/10.1128/JCM.00810-19. (PMID: 10.1128/JCM.00810-19316195256935912) Brasil (2008) Ministério da Saúde. Secretaria de Vigilância em Saúde. Departamento de Vigilância Epidemiológica. Manual nacional de vigilância laboratorial da tuberculose e outras micobactérias. Brasília (DF): Ministério da Saúde 436. [Série A. Normas e Manuais Técnicos]. Fadzilah MN, Kee Peng NG, Yun Fong N (2009) The manual MGIT system for the detection of M. tuberculosis in respiratory specimens: an experience in the University Malaya Medical Centre. J Pathol 31(2):93–97. Maruthai K, Ravibalan T, Vallayyachari K, Kesavan S, Samrot AV, Uthaiah M (2015) Molecular typing and differentiation of Mycobacterium tuberculosis clinical isolates using Double Repetitive Element PCR and Duplex PCR. Int J Mycobacteriol 4(1):60–66. https://doi.org/10.1016/j.ijmyco.2014.11.061. (PMID: 10.1016/j.ijmyco.2014.11.06126655200) BACTEC MGIT 960™ system, Becton, Dickinson, Sparks, MD, USA. https://legacy.bd.com/ds/technicalCenter/inserts/8809501JAA(03).pdf . Accessed August 2020. Asandem DA, Asante-Poku A, Asare P, Aboagye SY, Stephen OW, Danso E, Klevor PM, Hayibor KM, Yeboah-Manu D (2018) OMNIgene • SPUTUM: a good transport and decontaminating reagent for tuberculosis testing. Int J Mycobacteriol 7(3):222–227. https://doi.org/10.4103/ijmy.ijmy_102_18. (PMID: 10.4103/ijmy.ijmy_102_1830198500) Azam K, Cadir N, Madeira C, Gillespie SH, Sabiiti W (2018) OMNIgene • SPUTUM suppresses contaminants while maintaining Mycobacterium tuberculosis viability and obviates cold-chain transport. ERJ Res 4(1):00074–02017. https://doi.org/10.1183/23120541.00074-2017. (PMID: 10.1183/23120541.00074-2017) Alemayehu WA, Neri S, Dalebout S, Nalikungwi R, Trusov A, Ahmed E, Dimba A, Weirich A, Curry PS, Kelly-Cirino CD (2019) Comparative study of OMNIgene®•SPUTUM reagent versus cold-chain for the transportation of sputum samples to GeneXpert®MTBC/RIF testing sites in Malawi. BMC Infect Dis 19:424. https://doi.org/10.1186/s12879-019-4070-8. (PMID: 10.1186/s12879-019-4070-8310969456521431) Olaru ID, Heyckendorf J, Grossmann S, Lange C (2014) Time to culture positivity and sputum smear microscopyduring tuberculosis therapy. PLoS One 9(8):e106075. https://doi.org/10.1371/journal.pone.0106075. (PMID: 10.1371/journal.pone.0106075251713374149502) Bimba JS, Lawson L, Kontogianni K, Edwards T, Ekpenyong BE, Dodd J, Adams ER, Sloan DJ, Creswell J, Dominguez J, Cuevas LE (2019) PrimeStore MTM and OMNIgene • SPUTUM for the preservation of sputum for Xpert MTB/RIF testing in Nigeria. J Clin Med 8(12):2146. https://doi.org/10.3390/jcm8122146. (PMID: 10.3390/jcm81221466947270) Lawn SD, Nicol MP (2011) Xpert® MTB/RIF assay: development, evaluation and implementation of a new rapid molecular diagnostic for tuberculosis and rifampicin resistance. Future Microbiol 6(9):1067–1082. https://doi.org/10.2217/fmb.11.84. (PMID: 10.2217/fmb.11.8421958145) Tagliani E, Alagna R, Tafaj S, Hafizi H, Cirillo DM (2017) Evaluation of Mycobacterium tuberculosis viability in OMNIgene • SPUTUM reagent upon multi-day transport at ambient temperature. BMC Infect Dis 17(1):663. https://doi.org/10.1186/s12879-017-2756-3. (PMID: 10.1186/s12879-017-2756-3289696005625711) |
| فهرسة مساهمة: | Keywords: BACTEC MGIT; OMNIgene • SPUTUM; Sputum transport; Tuberculosis; Xpert MTB/RIF |
| المشرفين على المادة: | 0 (Indicators and Reagents) |
| تواريخ الأحداث: | Date Created: 20210823 Date Completed: 20211215 Latest Revision: 20220824 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8578262 |
| DOI: | 10.1007/s42770-021-00568-2 |
| PMID: | 34424510 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1678-4405 |
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| DOI: | 10.1007/s42770-021-00568-2 |