دورية أكاديمية
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The method to quantify cell elasticity based on the precise measurement of pressure inducing cell deformation in microfluidic channels.

التفاصيل البيبلوغرافية
العنوان: The method to quantify cell elasticity based on the precise measurement of pressure inducing cell deformation in microfluidic channels.
المؤلفون: Chen Z; School of Mechanical Engineering and Automation, Harbin Institute of Technology, Shenzhen, Shenzhen 518055, China., Yip TF; School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam Hong Kong, China.; Laboratory of Data Discovery for Health Limited (D24H), Hong Kong Science Park, New Territory, Hong Kong, China., Zhu Y; School of Mechanical Engineering and Automation, Harbin Institute of Technology, Shenzhen, Shenzhen 518055, China., Ho JWK; School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam Hong Kong, China.; Laboratory of Data Discovery for Health Limited (D24H), Hong Kong Science Park, New Territory, Hong Kong, China., Chen H; School of Mechanical Engineering and Automation, Harbin Institute of Technology, Shenzhen, Shenzhen 518055, China.
المصدر: MethodsX [MethodsX] 2021 Jan 23; Vol. 8, pp. 101247. Date of Electronic Publication: 2021 Jan 23 (Print Publication: 2021).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101639829 Publication Model: eCollection Cited Medium: Print ISSN: 2215-0161 (Print) Linking ISSN: 22150161 NLM ISO Abbreviation: MethodsX Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier B.V., [2014]-
مستخلص: The cell elasticity has attracted extensive research interests since it not only provides new insights into cell biology but also is an emerging mechanical marker for the diagnosis of some diseases. This paper reports the method for the precise measurement of mechanical properties of single cells deformed to a large extent using a novel microfluidic system integrated with a pressure feedback system and small particle separation unit. The particle separation system was employed to avoid the blockage of the cell deformation channel to enhance the measurement throughput. This system is of remarkable application potential in the precise evaluation of cell mechanical properties. In brief, this paper reports:•The manufacturing of the chip using standard soft lithography;•The methods to deform single cells in a microchannel and measure the relevant pressure drop using a pressure sensor connecting to the microfluidic chip;•Calculation of the mechanical properties including stiffness and fluidity of each cell based on a power-law rheology model describing the viscoelastic behaviors of cells;•Automatic and real-time measurement of the mechanical properties using video processing software.
Competing Interests: The Authors confirm that there are no conflicts of interest.
(© 2021 The Authors. Published by Elsevier B.V.)
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فهرسة مساهمة: Keywords: Cell deformation; Cell elasticity; Measurement; Microfluidic; Pressure drop; Video processing
تواريخ الأحداث: Date Created: 20210826 Latest Revision: 20210827
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8374187
DOI: 10.1016/j.mex.2021.101247
PMID: 34434770
قاعدة البيانات: MEDLINE
الوصف
تدمد:2215-0161
DOI:10.1016/j.mex.2021.101247