دورية أكاديمية
أعرض في EDS

Tracheal aspirate RNA sequencing identifies distinct immunological features of COVID-19 ARDS.

التفاصيل البيبلوغرافية
العنوان: Tracheal aspirate RNA sequencing identifies distinct immunological features of COVID-19 ARDS.
المؤلفون: Sarma A; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA., Christenson SA; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA., Byrne A; Chan Zuckerberg Biohub, San Francisco, CA, USA., Mick E; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA.; Chan Zuckerberg Biohub, San Francisco, CA, USA.; Division of Infectious Diseases, University of California, San Francisco, CA, USA., Pisco AO; Chan Zuckerberg Biohub, San Francisco, CA, USA., DeVoe C; Division of Infectious Diseases, University of California, San Francisco, CA, USA., Deiss T; Chan Zuckerberg Biohub, San Francisco, CA, USA., Ghale R; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA.; Division of Infectious Diseases, University of California, San Francisco, CA, USA., Zha BS; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA., Tsitsiklis A; Division of Infectious Diseases, University of California, San Francisco, CA, USA., Jauregui A; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA., Moazed F; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA., Detweiler AM; Division of Infectious Diseases, University of California, San Francisco, CA, USA., Spottiswoode N; Department of Medicine, University of California, San Francisco, CA, USA., Sinha P; Department of Anesthesia, Washington University, Saint Louis, MO, USA., Neff N; Chan Zuckerberg Biohub, San Francisco, CA, USA., Tan M; Chan Zuckerberg Biohub, San Francisco, CA, USA., Serpa PH; Division of Infectious Diseases, University of California, San Francisco, CA, USA., Willmore A; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA., Ansel KM; Department of Microbiology and Immunology, University of California, San Francisco, CA, USA.; Sandler Asthma Basic Research Center, University of California, San Francisco, CA, USA., Wilson JG; Department of Emergency Medicine, Stanford University, Palo Alto, CA, USA., Leligdowicz A; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA.; Interdepartmental Division of Critical Care Medicine, University of Toronto, Toronto, Ontario, Canada.; Cardiovascular Research Institute, University of California, San Francisco, CA, USA., Siegel ER; School of Medicine, University of California, San Francisco, CA, USA., Sirota M; Division of Rheumatology, University of California, San Francisco, CA, USA., DeRisi JL; Chan Zuckerberg Biohub, San Francisco, CA, USA.; Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA., Matthay MA; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA.; Cardiovascular Research Institute, University of California, San Francisco, CA, USA.; Department of Anesthesia, University of California, San Francisco, CA, USA., Hendrickson CM; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA., Kangelaris KN; Department of Medicine, University of California, San Francisco, CA, USA., Krummel MF; Department of Pathology, University of California, San Francisco, CA, USA., Woodruff PG; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA.; Sandler Asthma Basic Research Center, University of California, San Francisco, CA, USA., Erle DJ; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA.; Department of Medicine, University of California, San Francisco, CA, USA.; Lung Biology Center, University of California, San Francisco, CA, USA.; UCSF CoLabs, University of California, San Francisco, CA, USA., Calfee CS; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California, San Francisco, CA, USA.; Cardiovascular Research Institute, University of California, San Francisco, CA, USA.; Department of Anesthesia, University of California, San Francisco, CA, USA., Langelier CR; Chan Zuckerberg Biohub, San Francisco, CA, USA. chaz.langelier@ucsf.edu.; Division of Infectious Diseases, University of California, San Francisco, CA, USA. chaz.langelier@ucsf.edu.
مؤلفون مشاركون: COMET Consortium
المصدر: Nature communications [Nat Commun] 2021 Aug 26; Vol. 12 (1), pp. 5152. Date of Electronic Publication: 2021 Aug 26.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: COVID-19/*genetics , RNA/*genetics , Respiratory Distress Syndrome/*genetics , Trachea/*immunology, Adult ; Aged ; Aged, 80 and over ; COVID-19/immunology ; COVID-19/virology ; Case-Control Studies ; Cohort Studies ; Critical Illness ; Cytokines/genetics ; Cytokines/immunology ; Female ; Gene Expression Profiling ; Humans ; Male ; Middle Aged ; RNA/metabolism ; Respiratory Distress Syndrome/immunology ; Respiratory Distress Syndrome/virology ; SARS-CoV-2/physiology ; Sequence Analysis, RNA
مستخلص: The immunological features that distinguish COVID-19-associated acute respiratory distress syndrome (ARDS) from other causes of ARDS are incompletely understood. Here, we report the results of comparative lower respiratory tract transcriptional profiling of tracheal aspirate from 52 critically ill patients with ARDS from COVID-19 or from other etiologies, as well as controls without ARDS. In contrast to a "cytokine storm," we observe reduced proinflammatory gene expression in COVID-19 ARDS when compared to ARDS due to other causes. COVID-19 ARDS is characterized by a dysregulated host response with increased PTEN signaling and elevated expression of genes with non-canonical roles in inflammation and immunity. In silico analysis of gene expression identifies several candidate drugs that may modulate gene expression in COVID-19 ARDS, including dexamethasone and granulocyte colony stimulating factor. Compared to ARDS due to other types of viral pneumonia, COVID-19 is characterized by impaired interferon-stimulated gene (ISG) expression. The relationship between SARS-CoV-2 viral load and expression of ISGs is decoupled in patients with COVID-19 ARDS when compared to patients with mild COVID-19. In summary, assessment of host gene expression in the lower airways of patients reveals distinct immunological features of COVID-19 ARDS.
(© 2021. The Author(s).)
التعليقات: Update of: Res Sq. 2021 Jan 14;:. (PMID: 33469573)
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معلومات مُعتمدة: R35 HL140026 United States HL NHLBI NIH HHS; K24 HL137013 United States HL NHLBI NIH HHS; K23 HL133495 United States HL NHLBI NIH HHS; K23 HL138461 United States HL NHLBI NIH HHS; F32 HL151117 United States HL NHLBI NIH HHS; U19 AI077439 United States AI NIAID NIH HHS
فهرسة مساهمة: Investigator: Y Abe-Jones; S Asthana; A Beagle; T Bhakta; S Bhide; C Cai; S Caldera; C Calfee; M Calvo; S Carrillo; A Cattamanchi; S Chak; V Chan; N Chew; S Christenson; Z Collins; A Combes; T Courau; S Darmanis; D Erle; A Esmaili; GK Fragiadakis; R Ghale; J Giberson; A Gonzalez; PH Serpa; C Hendrickson; K Hiam; K Hu; B Huang; A Jauregui; C Jones; N Jones; K Kangelaris; M Krummel; N Kumar; D Kushnoor; T Lea; D Lee; D Lee; KD Liu; Y Liu; S Mahboob; M Matthay; J Milush; P Muñoz-Sandoval; V Nguyen; G Ortiz; R Parada; M Phelps; L Pierce; P Prasad; A Rao; S Rashid; G Reeder; N Rodriguez; B Samad; D Scarlet; C Shaw; A Shen; A Sigman; M Spitzer; Y Sun; S Sunshine; K Tang; LT Altamirano; J Tsui; E Tumurbaatar; K Turner; A Ward; A Willmore; M Wilson; J Winkler; R Withers; K Wong; P Woodruff; J Ye; K Yee; M Yu; S Zha; J Zhan; M Zhou; WS Zhu
المشرفين على المادة: 0 (Cytokines)
63231-63-0 (RNA)
تواريخ الأحداث: Date Created: 20210827 Date Completed: 20210913 Latest Revision: 20231117
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8390461
DOI: 10.1038/s41467-021-25040-5
PMID: 34446707
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-021-25040-5