دورية أكاديمية
Considerations for understanding protein measurements: Identification of formation, degradation and more pathological relevant epitopes.
| العنوان: | Considerations for understanding protein measurements: Identification of formation, degradation and more pathological relevant epitopes. |
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| المؤلفون: | Karsdal MA; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark. Electronic address: mk@nordicbio.com., Genovese F; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark., Rasmussen DGK; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark., Bay-Jensen AC; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark., Mortensen JH; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark., Holm Nielsen S; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark., Willumsen N; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark., Jensen C; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark., Manon-Jensen T; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark., Jennings L; Novartis, Basel, Switzerland., Reese-Petersen AL; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark., Henriksen K; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark., Sand JM; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark., Bager C; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark., Leeming DJ; Nordic Bioscience, Biomarkers & Research A/S, Herlev, Denmark. |
| المصدر: | Clinical biochemistry [Clin Biochem] 2021 Nov; Vol. 97, pp. 11-24. Date of Electronic Publication: 2021 Aug 26. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 0133660 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2933 (Electronic) Linking ISSN: 00099120 NLM ISO Abbreviation: Clin Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tarrytown, NY : Elsevier Science Original Publication: [Toronto] Canadian Society of Clinical Chemists. |
| مواضيع طبية MeSH: | Epitopes*, Cardiovascular Diseases/*metabolism , Collagen/*immunology , Proteins/*analysis , Proteins/*metabolism, Basement Membrane/metabolism ; Bone Remodeling/immunology ; Collagen/analysis ; Collagen/metabolism ; Gastrointestinal Diseases/metabolism ; Humans ; Kidney/metabolism ; Liver Cirrhosis/metabolism ; Neoplasms/immunology ; Prognosis ; Protein Domains ; Protein Processing, Post-Translational ; Proteins/immunology |
| مستخلص: | Objectives: There is a need for precision medicine and an unspoken promise of an optimal approach for identification of the right patients for value-based medicine based on big data. However, there may be a misconception that measurement of proteins is more valuable than measurement of fewer selected biomarkers. In population-based research, variation may be somewhat eliminated by quantity. However, this fascination of numbers may limit the attention to and understanding of the single. This review highlights that protein measurements (with collagens as examples) may mean different things depending on the targeted epitope - formation or degradation of tissues, and even signaling potential of proteins. Design and Methods: PubMed was searched for collagen, neo-epitope, biomarkers. Results: Ample examples of assays with specific epitopes, either pathological such as HbA1c, or domain specific such as pro-peptides, which total protein arrays would not have identified were evident. Conclusions: We suggest that big data may be considered as the funnel of data points, in which most important parameters will be selected. If the technical precision is low or the biological accuracy is limited, and we include suboptimal quality of biomarkers, disguised as big data, we may not be able to fulfill the promise of helping patients searching for the optimal treatment. Alternatively, if the technical precision of the total protein quantification is high, but we miss the functional domains with the most considerable biological meaning, we miss the most important and valuable information of a given protein. This review highlights that measurements of the same protein in different ways may provide completely different meanings. We need to understand the pathological importance of each epitope quantified to maximize protein measurements. (Copyright © 2021 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Assay; Collagen; Degradation; Epitope; Formation; Neo-epitope; Protein |
| المشرفين على المادة: | 0 (Epitopes) 0 (Proteins) 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20210828 Date Completed: 20211227 Latest Revision: 20211227 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.clinbiochem.2021.08.007 |
| PMID: | 34453894 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2933 |
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| DOI: | 10.1016/j.clinbiochem.2021.08.007 |