دورية أكاديمية
Microbiological load and preantral follicle preservation using different systems for ovarian tissue vitrification in the red-rumped agouti.
| العنوان: | Microbiological load and preantral follicle preservation using different systems for ovarian tissue vitrification in the red-rumped agouti. |
|---|---|
| المؤلفون: | Praxedes ÉCG; Laboratory on Animal Germplasm Conservation, Universidade Federal Rural Do Semi-Árido (UFERSA), BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil., Bezerra LGP; Laboratory on Animal Germplasm Conservation, Universidade Federal Rural Do Semi-Árido (UFERSA), BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil., Moreira SSJ; Laboratory on Animal Germplasm Conservation, Universidade Federal Rural Do Semi-Árido (UFERSA), BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil., Santos CS; Laboratory on Animal Germplasm Conservation, Universidade Federal Rural Do Semi-Árido (UFERSA), BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil., Brasil AV; Laboratory on Animal Germplasm Conservation, Universidade Federal Rural Do Semi-Árido (UFERSA), BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil., Silva AMD; Laboratory on Animal Germplasm Conservation, Universidade Federal Rural Do Semi-Árido (UFERSA), BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil., Guerreiro DD; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculdade de Veterinária, Universidade Do Ceara, Av. Paranjana 1700, Campus Do Itaperi, 60714-903, Fortaleza, CE, Brazil., Pereira AF; Laboratory of Animal Biotechnology, UFERSA, BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil., Rodrigues APR; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculdade de Veterinária, Universidade Do Ceara, Av. Paranjana 1700, Campus Do Itaperi, 60714-903, Fortaleza, CE, Brazil., Silva AR; Laboratory on Animal Germplasm Conservation, Universidade Federal Rural Do Semi-Árido (UFERSA), BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil. Electronic address: alexrs@ufersa.edu.br. |
| المصدر: | Cryobiology [Cryobiology] 2021 Dec; Vol. 103, pp. 123-128. Date of Electronic Publication: 2021 Aug 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0006252 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2392 (Electronic) Linking ISSN: 00112240 NLM ISO Abbreviation: Cryobiology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : Amsterdam : Elsevier Original Publication: San Diego, CA : Academic Press |
| مواضيع طبية MeSH: | Dasyproctidae* , Vitrification*, Animals ; Cryopreservation/methods ; Cryoprotective Agents/pharmacology ; Female ; Humans ; Ovarian Follicle ; Tissue Preservation |
| مستخلص: | We evaluated the effect of open and closed systems used for ovarian tissue vitrification on the microbiological load and preservation of preantral follicles (PAFs) in the red-rumped agoutis. The ovaries from eight females were recovered and fragmented, with four cortexes fragments immediately fixed and evaluated (fresh group). The other fragments were processed for the solid-surface vitrification method (SSV) or an ovarian tissue cryosystem (OTC) using fetal calf serum, ethylene glycol, and sucrose as cryoprotectants, stored for two weeks, and rewarmed. Subsequently, fragments were subjected to a 24-h in vitro culture and assessed for microbiological load, PAF morphology, and DNA integrity. There was no fungal contamination; however, the vitrified samples from two individuals showed bacterial contamination of 79 200 colony forming units per milliliter (CFU)/mL for SSV and 3120 CFU/mL for OTC. From those samples, a total of eight different types of bacterial colonies were isolated and identified as coagulase-negative Staphylococci and Gram-positive bacilli. Regarding PAF morphology, both systems provided adequate preservation, with values higher than 70% normal follicles observed before and after culture. The TUNEL assay revealed that both SSV (52.39%) and OTC (41.67%) could preserve DNA integrity after vitrification and after 24 h of culture. In summary, both open and closed systems were equally efficient in preserving agouti ovarian tissues, especially concerning the preantral follicle morphology and DNA integrity; however, the OTC seems to provide a less adequate environment for bacterial proliferation. (Copyright © 2021 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Biobank; In vitro culture; Rodent; Wildlife |
| المشرفين على المادة: | 0 (Cryoprotective Agents) |
| تواريخ الأحداث: | Date Created: 20210829 Date Completed: 20220128 Latest Revision: 20220128 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.cryobiol.2021.08.003 |
| PMID: | 34454959 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2392 |
|---|---|
| DOI: | 10.1016/j.cryobiol.2021.08.003 |