دورية أكاديمية
DNase I improves blood-milk barrier integrity and alleviates inflammation induced by Staphylococcus aureus during mastitis.
| العنوان: | DNase I improves blood-milk barrier integrity and alleviates inflammation induced by Staphylococcus aureus during mastitis. |
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| المؤلفون: | Jingjing W; College of Life Science and Engineering, Foshan University, Foshan, Guangdong 528231, People's Republic of China., Yiwu F; College of Life Science and Engineering, Foshan University, Foshan, Guangdong 528231, People's Republic of China., Youpeng S; College of Life Science and Engineering, Foshan University, Foshan, Guangdong 528231, People's Republic of China., Xia W; College of Life Science and Engineering, Foshan University, Foshan, Guangdong 528231, People's Republic of China., Zhikai W; College of Life Science and Engineering, Foshan University, Foshan, Guangdong 528231, People's Republic of China., Peixuan L; College of Life Science and Engineering, Foshan University, Foshan, Guangdong 528231, People's Republic of China. Electronic address: lpx6393@163.com., Ershun Z; College of Life Science and Engineering, Foshan University, Foshan, Guangdong 528231, People's Republic of China. Electronic address: zhouershun594926@163.com., Zhengtao Y; College of Life Science and Engineering, Foshan University, Foshan, Guangdong 528231, People's Republic of China. Electronic address: yangzhengtao01@sina.com. |
| المصدر: | International immunopharmacology [Int Immunopharmacol] 2021 Nov; Vol. 100, pp. 108079. Date of Electronic Publication: 2021 Aug 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 100965259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1705 (Electronic) Linking ISSN: 15675769 NLM ISO Abbreviation: Int Immunopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier Science, c2001- |
| مواضيع طبية MeSH: | Deoxyribonuclease I/*pharmacology , Mastitis/*drug therapy , Staphylococcal Infections/*drug therapy, Animals ; Deoxyribonuclease I/therapeutic use ; Female ; Humans ; Lipopolysaccharides/administration & dosage ; Lipopolysaccharides/immunology ; Mammary Glands, Animal/blood supply ; Mammary Glands, Animal/immunology ; Mammary Glands, Animal/microbiology ; Mammary Glands, Animal/pathology ; Mastitis/immunology ; Mastitis/microbiology ; Mastitis/pathology ; Mice ; Signal Transduction/drug effects ; Signal Transduction/immunology ; Staphylococcal Infections/immunology ; Staphylococcal Infections/pathology ; Staphylococcus aureus/immunology |
| مستخلص: | Mastitis is an inflammation of mammary gland, which directly affects the milk production performance and causes huge economic losses in the dairy industry. During mastitis, the blood-milk barrier (BMB) loses its integrity and aggravates the severity of mastitis. Exogenous DNase I has been exerted protective effects in different model of tissue injury. Here, we designed a study to investigate the effects of DNase I on inflammation and BMB in a mice model of Staphylococcus aureus-induced mastitis. In the model, we found that DNase I treatment significantly alleviated the inflammatory response through decrease of inflammatory cells in mammary alveoli, MPO activity and cytokines in mammary gland. Furthermore, immunofluorescent staining and western blotting demonstrated that exogenous DNase I obviously reduced BMB permeability and changed the expression of tight junction proteins to support the re-establishment of the barrier integrity. Mechanismly, DNase I treatment inhibited NF-κB and enhanced AKT signaling pathways. Therefore, our results indicate that DNase I may be an effective treatment for attenuating mastitis. (Copyright © 2021. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Blood-milk barrier; DNase I; Mastitis; Staphylococcus aureus |
| المشرفين على المادة: | 0 (Lipopolysaccharides) EC 3.1.21.1 (Deoxyribonuclease I) |
| تواريخ الأحداث: | Date Created: 20210829 Date Completed: 20220207 Latest Revision: 20220207 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.intimp.2021.108079 |
| PMID: | 34455255 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-1705 |
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| DOI: | 10.1016/j.intimp.2021.108079 |