دورية أكاديمية
Ex vivo Culture of Adult Mouse Antral Glands.
| العنوان: | Ex vivo Culture of Adult Mouse Antral Glands. |
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| المؤلفون: | Vallone VF; Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Faculty of Medicine, Université Libre de Bruxelles ULB, Brussels, Belgium., Leprovots M; Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Faculty of Medicine, Université Libre de Bruxelles ULB, Brussels, Belgium., Vassart G; Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Faculty of Medicine, Université Libre de Bruxelles ULB, Brussels, Belgium., Garcia MI; Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Faculty of Medicine, Université Libre de Bruxelles ULB, Brussels, Belgium. |
| المصدر: | Bio-protocol [Bio Protoc] 2017 Jan 05; Vol. 7 (1), pp. e2088. Date of Electronic Publication: 2017 Jan 05 (Print Publication: 2017). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bio-protocol LLC Country of Publication: United States NLM ID: 101635102 Publication Model: eCollection Cited Medium: Internet ISSN: 2331-8325 (Electronic) Linking ISSN: 23318325 NLM ISO Abbreviation: Bio Protoc Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Sunnyvale, CA, USA : Bio-protocol LLC, [2011]- |
| مستخلص: | The tri-dimensional culture, initially described by Sato et al. (2009) in order to isolate and characterize epithelial stem cells of the adult small intestine, has been subsequently adapted to many different organs. One of the first examples was the isolation and culture of antral stem cells by Barker et al. (2010), who efficiently generated organoids that recapitulate the mature pyloric epithelium in vitro . This ex vivo approach is suitable and promising to study gastric function in homeostasis as well as in disease. We have adapted Barker's protocol to compare homeostatic and regenerating tissues and here, we meticulously describe, step by step, the isolation and culture of antral glands as well as the isolation of single cells from antral glands that might be useful for culture after cell sorting as an example (Fernandez Vallone et al. , 2016 ). (Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.) |
| References: | Nature. 2009 May 14;459(7244):262-5. (PMID: 19329995) Cell Stem Cell. 2010 Jan 8;6(1):25-36. (PMID: 20085740) Development. 2016 May 1;143(9):1452-63. (PMID: 26989172) |
| فهرسة مساهمة: | Keywords: 3D; Antral glands; Gastric organoids; Murine gastric epithelium; Single cell; ex vivo |
| تواريخ الأحداث: | Date Created: 20210830 Latest Revision: 20220210 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8376483 |
| DOI: | 10.21769/BioProtoc.2088 |
| PMID: | 34458418 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2331-8325 |
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| DOI: | 10.21769/BioProtoc.2088 |