دورية أكاديمية
Ex vivo Culture of Fetal Mouse Gastric Epithelial Progenitors.
| العنوان: | Ex vivo Culture of Fetal Mouse Gastric Epithelial Progenitors. |
|---|---|
| المؤلفون: | Vallone VF; Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Faculty of Medicine, Université Libre de Bruxelles ULB, Brussels, Belgium., Leprovots M; Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Faculty of Medicine, Université Libre de Bruxelles ULB, Brussels, Belgium., Vassart G; Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Faculty of Medicine, Université Libre de Bruxelles ULB, Brussels, Belgium., Garcia MI; Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Faculty of Medicine, Université Libre de Bruxelles ULB, Brussels, Belgium. |
| المصدر: | Bio-protocol [Bio Protoc] 2017 Jan 05; Vol. 7 (1), pp. e2089. Date of Electronic Publication: 2017 Jan 05 (Print Publication: 2017). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bio-protocol LLC Country of Publication: United States NLM ID: 101635102 Publication Model: eCollection Cited Medium: Internet ISSN: 2331-8325 (Electronic) Linking ISSN: 23318325 NLM ISO Abbreviation: Bio Protoc Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Sunnyvale, CA, USA : Bio-protocol LLC, [2011]- |
| مستخلص: | Isolation and tridimensional culture of murine fetal progenitors from the digestive tract represents a new approach to study the nature and the biological characteristics of these epithelial cells that are present before the onset of the cytodifferentiation process during development. In 2013, Mustata et al. described the isolation of intestinal fetal progenitors growing as spheroids in the ex vivo culture system initially implemented by Sato et al. (2009) to grow adult intestinal stem cells. Noteworthy, fetal-derived spheroids have high self-renewal capacity making easy their indefinite maintenance in culture. Here, we report an adapted protocol for isolation and ex vivo culture and maintenance of fetal epithelial progenitors from distal pre-glandular stomach growing as gastric spheroids (Fernandez Vallone et al. , 2016 ). (Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.) |
| References: | Nature. 2009 May 14;459(7244):262-5. (PMID: 19329995) Cell Stem Cell. 2010 Jan 8;6(1):25-36. (PMID: 20085740) Cell Rep. 2013 Oct 31;5(2):421-32. (PMID: 24139799) Development. 2016 May 1;143(9):1452-63. (PMID: 26989172) |
| فهرسة مساهمة: | Keywords: 3D; Mouse fetal epithelium; Progenitors; Single cells; Spheroids; ex vivo |
| تواريخ الأحداث: | Date Created: 20210830 Latest Revision: 20220210 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8376515 |
| DOI: | 10.21769/BioProtoc.2089 |
| PMID: | 34458419 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2331-8325 |
|---|---|
| DOI: | 10.21769/BioProtoc.2089 |