دورية أكاديمية
Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation.
العنوان: | Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation. |
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المؤلفون: | Hoefig KP; Research Unit Molecular Immune Regulation, Helmholtz Center Munich, Munich, Germany., Reim A; Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Munich, Germany., Gallus C; Institute of Diabetes and Obesity, Helmholtz Center Munich, Munich, Germany., Wong EH; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany., Behrens G; Research Unit Molecular Immune Regulation, Helmholtz Center Munich, Munich, Germany., Conrad C; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany., Xu M; Research Unit Molecular Immune Regulation, Helmholtz Center Munich, Munich, Germany., Kifinger L; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany., Ito-Kureha T; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany., Defourny KAY; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany.; Department of Biomolecular Health Sciences, Utrecht University, Utrecht, The Netherlands., Geerlof A; Institute of Structural Biology, Helmholtz Center Munich, Neuherberg, Germany., Mautner J; Research Unit Gene Vectors, Helmholtz Center Munich & Children's Hospital, TU Munich, Munich, Germany., Hauck SM; Research Unit Protein Science, Helmholtz Center Munich, Munich, Germany., Baumjohann D; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany.; Medical Clinic III for Oncology, Immuno-Oncology and Rheumatology University Hospital Bonn, University of Bonn, Bonn, Germany., Feederle R; Monoclonal Antibody Core Facility and Research Group, Institute for Diabetes and Obesity, Helmholtz Center Munich, Neuherberg, Germany., Mann M; Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Munich, Germany., Wierer M; Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Munich, Germany. wierer@biochem.mpg.de.; Proteomics Research Infrastructure, University of Copenhagen, Copenhagen, Denmark. wierer@biochem.mpg.de., Glasmacher E; Institute of Diabetes and Obesity, Helmholtz Center Munich, Munich, Germany. elke.glasmacher@roche.com.; Roche Pharma Research and Early Development, Large Molecule Research, Roche Innovation Center Munich, Penzberg, Germany. elke.glasmacher@roche.com., Heissmeyer V; Research Unit Molecular Immune Regulation, Helmholtz Center Munich, Munich, Germany. vigo.heissmeyer@med.uni-muenchen.de.; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany. vigo.heissmeyer@med.uni-muenchen.de. |
المصدر: | Nature communications [Nat Commun] 2021 Sep 01; Vol. 12 (1), pp. 5208. Date of Electronic Publication: 2021 Sep 01. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [London] : Nature Pub. Group |
مواضيع طبية MeSH: | RNA, Messenger/*metabolism , RNA-Binding Proteins/*genetics , RNA-Binding Proteins/*metabolism , T-Lymphocytes, Helper-Inducer/*metabolism, Animals ; DNA-Binding Proteins ; Gene Expression Regulation ; HEK293 Cells ; Humans ; Inducible T-Cell Co-Stimulator Protein/genetics ; Mice ; Proto-Oncogene Proteins c-vav ; STAT1 Transcription Factor ; STAT4 Transcription Factor ; Signal Transduction ; Trans-Activators/metabolism ; Ubiquitin-Protein Ligases/genetics |
مستخلص: | Post-transcriptional gene regulation in T cells is dynamic and complex as targeted transcripts respond to various factors. This is evident for the Icos mRNA encoding an essential costimulatory receptor that is regulated by several RNA-binding proteins (RBP), including Roquin-1 and Roquin-2. Here, we identify a core RBPome of 798 mouse and 801 human T cell proteins by utilizing global RNA interactome capture (RNA-IC) and orthogonal organic phase separation (OOPS). The RBPome includes Stat1, Stat4 and Vav1 proteins suggesting unexpected functions for these transcription factors and signal transducers. Based on proximity to Roquin-1, we select ~50 RBPs for testing coregulation of Roquin-1/2 targets by induced expression in wild-type or Roquin-1/2-deficient T cells. Besides Roquin-independent contributions from Rbms1 and Cpeb4 we also show Roquin-1/2-dependent and target-specific coregulation of Icos by Celf1 and Igf2bp3. Connecting the cellular RBPome in a post-transcriptional context, we find contributions from multiple RBPs to the prototypic regulation of mRNA targets by individual trans-acting factors. (© 2021. The Author(s).) |
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المشرفين على المادة: | 0 (CPEB4 protein, human) 0 (DNA-Binding Proteins) 0 (ICOS protein, human) 0 (Icos protein, mouse) 0 (Inducible T-Cell Co-Stimulator Protein) 0 (Proto-Oncogene Proteins c-vav) 0 (RBMS1 protein, human) 0 (RNA, Messenger) 0 (RNA-Binding Proteins) 0 (STAT1 Transcription Factor) 0 (STAT4 Transcription Factor) 0 (Trans-Activators) 0 (VAV1 protein, human) EC 2.3.2.27 (Rc3h1 protein, mouse) EC 2.3.2.27 (Ubiquitin-Protein Ligases) |
تواريخ الأحداث: | Date Created: 20210902 Date Completed: 20210920 Latest Revision: 20210923 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC8410761 |
DOI: | 10.1038/s41467-021-25345-5 |
PMID: | 34471108 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2041-1723 |
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DOI: | 10.1038/s41467-021-25345-5 |