دورية أكاديمية
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Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation.

التفاصيل البيبلوغرافية
العنوان: Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation.
المؤلفون: Hoefig KP; Research Unit Molecular Immune Regulation, Helmholtz Center Munich, Munich, Germany., Reim A; Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Munich, Germany., Gallus C; Institute of Diabetes and Obesity, Helmholtz Center Munich, Munich, Germany., Wong EH; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany., Behrens G; Research Unit Molecular Immune Regulation, Helmholtz Center Munich, Munich, Germany., Conrad C; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany., Xu M; Research Unit Molecular Immune Regulation, Helmholtz Center Munich, Munich, Germany., Kifinger L; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany., Ito-Kureha T; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany., Defourny KAY; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany.; Department of Biomolecular Health Sciences, Utrecht University, Utrecht, The Netherlands., Geerlof A; Institute of Structural Biology, Helmholtz Center Munich, Neuherberg, Germany., Mautner J; Research Unit Gene Vectors, Helmholtz Center Munich & Children's Hospital, TU Munich, Munich, Germany., Hauck SM; Research Unit Protein Science, Helmholtz Center Munich, Munich, Germany., Baumjohann D; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany.; Medical Clinic III for Oncology, Immuno-Oncology and Rheumatology University Hospital Bonn, University of Bonn, Bonn, Germany., Feederle R; Monoclonal Antibody Core Facility and Research Group, Institute for Diabetes and Obesity, Helmholtz Center Munich, Neuherberg, Germany., Mann M; Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Munich, Germany., Wierer M; Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Munich, Germany. wierer@biochem.mpg.de.; Proteomics Research Infrastructure, University of Copenhagen, Copenhagen, Denmark. wierer@biochem.mpg.de., Glasmacher E; Institute of Diabetes and Obesity, Helmholtz Center Munich, Munich, Germany. elke.glasmacher@roche.com.; Roche Pharma Research and Early Development, Large Molecule Research, Roche Innovation Center Munich, Penzberg, Germany. elke.glasmacher@roche.com., Heissmeyer V; Research Unit Molecular Immune Regulation, Helmholtz Center Munich, Munich, Germany. vigo.heissmeyer@med.uni-muenchen.de.; Institute for Immunology, Biomedical Center, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany. vigo.heissmeyer@med.uni-muenchen.de.
المصدر: Nature communications [Nat Commun] 2021 Sep 01; Vol. 12 (1), pp. 5208. Date of Electronic Publication: 2021 Sep 01.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: RNA, Messenger/*metabolism , RNA-Binding Proteins/*genetics , RNA-Binding Proteins/*metabolism , T-Lymphocytes, Helper-Inducer/*metabolism, Animals ; DNA-Binding Proteins ; Gene Expression Regulation ; HEK293 Cells ; Humans ; Inducible T-Cell Co-Stimulator Protein/genetics ; Mice ; Proto-Oncogene Proteins c-vav ; STAT1 Transcription Factor ; STAT4 Transcription Factor ; Signal Transduction ; Trans-Activators/metabolism ; Ubiquitin-Protein Ligases/genetics
مستخلص: Post-transcriptional gene regulation in T cells is dynamic and complex as targeted transcripts respond to various factors. This is evident for the Icos mRNA encoding an essential costimulatory receptor that is regulated by several RNA-binding proteins (RBP), including Roquin-1 and Roquin-2. Here, we identify a core RBPome of 798 mouse and 801 human T cell proteins by utilizing global RNA interactome capture (RNA-IC) and orthogonal organic phase separation (OOPS). The RBPome includes Stat1, Stat4 and Vav1 proteins suggesting unexpected functions for these transcription factors and signal transducers. Based on proximity to Roquin-1, we select ~50 RBPs for testing coregulation of Roquin-1/2 targets by induced expression in wild-type or Roquin-1/2-deficient T cells. Besides Roquin-independent contributions from Rbms1 and Cpeb4 we also show Roquin-1/2-dependent and target-specific coregulation of Icos by Celf1 and Igf2bp3. Connecting the cellular RBPome in a post-transcriptional context, we find contributions from multiple RBPs to the prototypic regulation of mRNA targets by individual trans-acting factors.
(© 2021. The Author(s).)
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المشرفين على المادة: 0 (CPEB4 protein, human)
0 (DNA-Binding Proteins)
0 (ICOS protein, human)
0 (Icos protein, mouse)
0 (Inducible T-Cell Co-Stimulator Protein)
0 (Proto-Oncogene Proteins c-vav)
0 (RBMS1 protein, human)
0 (RNA, Messenger)
0 (RNA-Binding Proteins)
0 (STAT1 Transcription Factor)
0 (STAT4 Transcription Factor)
0 (Trans-Activators)
0 (VAV1 protein, human)
EC 2.3.2.27 (Rc3h1 protein, mouse)
EC 2.3.2.27 (Ubiquitin-Protein Ligases)
تواريخ الأحداث: Date Created: 20210902 Date Completed: 20210920 Latest Revision: 20210923
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8410761
DOI: 10.1038/s41467-021-25345-5
PMID: 34471108
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-021-25345-5