دورية أكاديمية
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Automated Phosphopeptide Enrichment for Gram-Positive Bacteria.

التفاصيل البيبلوغرافية
العنوان: Automated Phosphopeptide Enrichment for Gram-Positive Bacteria.
المؤلفون: Birk MS; Max Planck Unit for the Science of Pathogens, 10117 Berlin, Germany., Charpentier E; Max Planck Unit for the Science of Pathogens, 10117 Berlin, Germany., Frese CK; Max Planck Unit for the Science of Pathogens, 10117 Berlin, Germany.
المصدر: Journal of proteome research [J Proteome Res] 2021 Oct 01; Vol. 20 (10), pp. 4886-4892. Date of Electronic Publication: 2021 Sep 02.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101128775 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-3907 (Electronic) Linking ISSN: 15353893 NLM ISO Abbreviation: J Proteome Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, D.C. : American Chemical Society, c2002-
مواضيع طبية MeSH: Chromatography, Affinity* , Phosphopeptides*/metabolism, Bacillus subtilis/metabolism ; Listeria monocytogenes/metabolism ; Phosphorylation ; Proteome/metabolism ; Streptococcus pyogenes/metabolism ; Titanium
مستخلص: Protein phosphorylation in prokaryotes has gained more attention in recent years as several studies linked it to regulatory and signaling functions, indicating importance similar to protein phosphorylation in eukaryotes. Studies on bacterial phosphorylation have so far been conducted using manual or HPLC-supported phosphopeptide enrichment, whereas automation of phosphopeptide enrichment has been established in eukaryotes, allowing for high-throughput sampling. To facilitate the prospect of studying bacterial phosphorylation on a systems level, we here established an automated Ser/Thr/Tyr phosphopeptide enrichment workflow on the Agilent AssayMap platform. We present optimized buffer conditions for TiO 2 and Fe(III)-NTA-IMAC cartridge-based enrichment and the most advantageous, species-specific loading amounts for Streptococcus pyogenes , Listeria monocytogenes , and Bacillus subtilis . For higher sample amounts (≥250 μg), we observed superior performance of the Fe(III)-NTA cartridges, whereas for lower sample amounts (≤100 μg), TiO 2 -based enrichment is equally efficient. Both cartridges largely enriched the same set of phosphopeptides, suggesting no improvement of peptide yield by the complementary use of the two cartridges. Our data represent, to the best of our knowledge, the largest phosphoproteome identified in a single study for each of these bacteria.
References: BMC Genomics. 2008 Oct 16;9:488. (PMID: 18925949)
Nat Protoc. 2009;4(1):44-57. (PMID: 19131956)
FEMS Microbiol Rev. 2016 May;40(3):398-417. (PMID: 26926353)
Nat Methods. 2013 Dec;10(12):1211-2. (PMID: 24097270)
Proteomics. 2011 Nov;11(21):4155-65. (PMID: 21956863)
J Proteome Res. 2014 Dec 5;13(12):6046-57. (PMID: 25383790)
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450. (PMID: 30395289)
Biochim Biophys Acta. 2011 Oct;1810(10):989-94. (PMID: 21266190)
Int J Mol Sci. 2019 Nov 13;20(22):. (PMID: 31766156)
Mol Cell Proteomics. 2021 Jan 11;:100034. (PMID: 33444734)
Front Microbiol. 2019 Jun 19;10:1329. (PMID: 31275266)
PLoS One. 2018 Dec 10;13(12):e0208562. (PMID: 30532160)
J Proteome Res. 2014 Dec 5;13(12):6176-86. (PMID: 25338131)
Nat Rev Microbiol. 2019 Nov;17(11):651-664. (PMID: 31485032)
Philos Trans R Soc Lond B Biol Sci. 2012 Sep 19;367(1602):2513-6. (PMID: 22889903)
J Proteome Res. 2010 Jan;9(1):275-82. (PMID: 19894762)
Anal Chem. 2018 Aug 7;90(15):9529-9537. (PMID: 29969236)
Nucleic Acids Res. 2009 Jan;37(1):1-13. (PMID: 19033363)
Proteomics. 2011 Feb;11(4):554-70. (PMID: 21226000)
Expert Rev Proteomics. 2008 Aug;5(4):619-27. (PMID: 18761471)
Front Microbiol. 2020 Feb 04;10:3101. (PMID: 32117081)
J Proteome Res. 2017 Feb 3;16(2):728-737. (PMID: 28107008)
Mol Cell Proteomics. 2007 Apr;6(4):697-707. (PMID: 17218307)
Mol Cell Proteomics. 2014 Aug;13(8):1965-78. (PMID: 24390483)
Mol Cell Proteomics. 2008 Feb;7(2):299-307. (PMID: 17938405)
Mol Cell Proteomics. 2012 Nov;11(11):1191-202. (PMID: 22843994)
FEBS Lett. 2020 Aug;594(15):2339-2369. (PMID: 32337704)
Mol Cell Proteomics. 2016 May;15(5):1622-41. (PMID: 26912667)
فهرسة مساهمة: Keywords: BRAVO AssayMap; Bacillus subtilis; Fe(III)-IMAC; Listeria monocytogenes; Streptococcus pyogenes; TiO2; automation; bacterial phosphoproteomics; phosphopeptide enrichment
المشرفين على المادة: 0 (Phosphopeptides)
0 (Proteome)
D1JT611TNE (Titanium)
تواريخ الأحداث: Date Created: 20210902 Date Completed: 20211103 Latest Revision: 20220531
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8491273
DOI: 10.1021/acs.jproteome.1c00364
PMID: 34473931
قاعدة البيانات: MEDLINE
الوصف
تدمد:1535-3907
DOI:10.1021/acs.jproteome.1c00364