دورية أكاديمية
أعرض في EDS

Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni 2+ magnetic beads.

التفاصيل البيبلوغرافية
العنوان: Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni 2+ magnetic beads.
المؤلفون: Conzentino MS; Setor Litoral, UFPR Matinhos, PR, Brazil., Santos TPC; Setor Litoral, UFPR Matinhos, PR, Brazil., Selim KA; Organismic Interactions Department, Interfaculty Institute for Microbiology and Infection Medicine, Cluster of Excellence 'Controlling Microbes to Fight Infections', Tübingen University, Auf der Morgenstelle 28, 72076, Tübingen, Germany., Wagner B; Organismic Interactions Department, Interfaculty Institute for Microbiology and Infection Medicine, Cluster of Excellence 'Controlling Microbes to Fight Infections', Tübingen University, Auf der Morgenstelle 28, 72076, Tübingen, Germany., Alford JT; Organismic Interactions Department, Interfaculty Institute for Microbiology and Infection Medicine, Cluster of Excellence 'Controlling Microbes to Fight Infections', Tübingen University, Auf der Morgenstelle 28, 72076, Tübingen, Germany., Deobald N; Organismic Interactions Department, Interfaculty Institute for Microbiology and Infection Medicine, Cluster of Excellence 'Controlling Microbes to Fight Infections', Tübingen University, Auf der Morgenstelle 28, 72076, Tübingen, Germany., Paula NM; Setor Litoral, UFPR Matinhos, PR, Brazil., Rego FGM; Post-Graduation Program in Pharmaceutical Sciences, UFPR, Curitiba, PR, Brazil., Zanette DL; Instituto Carlos Chagas, FioCruz, PR, Brazil., Aoki MN; Instituto Carlos Chagas, FioCruz, PR, Brazil., Nardin JM; Hospital Erasto Gaertner, Curitiba, PR, Brazil., Huergo MCC; Setor Litoral, UFPR Matinhos, PR, Brazil., Reis RA; Department of Cell Biology, UFPR, Curitiba, PR, Brazil., Huergo LF; Setor Litoral, UFPR Matinhos, PR, Brazil. Electronic address: luciano.huergo@gmail.com.
المصدر: Analytical biochemistry [Anal Biochem] 2021 Oct 15; Vol. 631, pp. 114360. Date of Electronic Publication: 2021 Sep 03.
نوع المنشور: Evaluation Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0370535 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0309 (Electronic) Linking ISSN: 00032697 NLM ISO Abbreviation: Anal Biochem Subsets: MEDLINE
أسماء مطبوعة: Publication: <2000- > : San Diego, CA : Elsevier
Original Publication: Orlando Fl : Academic Press
مواضيع طبية MeSH: Antibodies, Viral/*blood , COVID-19/*diagnosis , COVID-19 Serological Testing/*methods , Immunoglobulin G/*blood , SARS-CoV-2/*isolation & purification, Antibodies, Viral/immunology ; Antigens, Viral/blood ; Antigens, Viral/immunology ; COVID-19/blood ; COVID-19/immunology ; COVID-19 Serological Testing/economics ; Enzyme-Linked Immunosorbent Assay/economics ; Enzyme-Linked Immunosorbent Assay/methods ; Humans ; Immunoglobulin G/immunology ; Magnets/chemistry ; Nickel/chemistry ; SARS-CoV-2/immunology ; Sensitivity and Specificity ; Seroconversion ; Time Factors
مستخلص: To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni 2+ magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
References: Viruses. 2020 Aug 07;12(8):. (PMID: 32784757)
J Infect Dis. 2020 Oct 1;222(9):1452-1461. (PMID: 32766833)
Lancet Infect Dis. 2020 Dec;20(12):1390-1400. (PMID: 32979318)
ACS Sens. 2021 Mar 26;6(3):703-708. (PMID: 33496577)
Nat Rev Microbiol. 2021 Mar;19(3):141-154. (PMID: 33024307)
Braz J Microbiol. 2021 Dec;52(4):2069-2073. (PMID: 34342836)
Emerg Infect Dis. 2020 Jul;26(7):1478-1488. (PMID: 32267220)
Nat Commun. 2020 Sep 17;11(1):4704. (PMID: 32943637)
ACS Cent Sci. 2020 May 27;6(5):591-605. (PMID: 32382657)
فهرسة مساهمة: Keywords: COVID-19; High throughput; Magnetic ELISA; Magnetic beads; SARS-CoV-2
المشرفين على المادة: 0 (Antibodies, Viral)
0 (Antigens, Viral)
0 (Immunoglobulin G)
7OV03QG267 (Nickel)
تواريخ الأحداث: Date Created: 20210905 Date Completed: 20211018 Latest Revision: 20221116
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8413102
DOI: 10.1016/j.ab.2021.114360
PMID: 34481802
قاعدة البيانات: MEDLINE
الوصف
تدمد:1096-0309
DOI:10.1016/j.ab.2021.114360