دورية أكاديمية
أعرض في EDS

A complete workflow for discovering small bioactive peptides in foods by LC-MS/MS: A case study on almonds.

التفاصيل البيبلوغرافية
العنوان: A complete workflow for discovering small bioactive peptides in foods by LC-MS/MS: A case study on almonds.
المؤلفون: Huang YP; Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA 95616, United States. Electronic address: yphhuang@ucdavis.edu., Dias FFG; Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA 95616, United States., Leite Nobrega de Moura Bell JM; Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA 95616, United States; Department of Biological and Agricultural Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, United States., Barile D; Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA 95616, United States; Foods for Health Institute, University of California, Davis, One Shields Avenue, Davis, CA 95616, United States. Electronic address: dbarile@ucdavis.edu.
المصدر: Food chemistry [Food Chem] 2022 Feb 01; Vol. 369, pp. 130834. Date of Electronic Publication: 2021 Aug 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE
أسماء مطبوعة: Publication: Barking : Elsevier Applied Science Publishers
Original Publication: Barking, Eng., Applied Science Publishers.
مواضيع طبية MeSH: Prunus dulcis*, Chromatography, Liquid ; Peptides ; Tandem Mass Spectrometry ; Workflow
مستخلص: Identification of bioactive peptides is an increasingly important target for food chemists, particularly in consideration of the widespread application of proteolytic enzymes in food processing. Because the characterization of small peptides by LC-MS/MS is challenging, we optimized a dimethyl labeling technique to facilitate small peptide identification, using almond proteins as a model. The method was validated by comparing the MS/MS spectra of standards and almond-derived peptides in their nonderivatized and derivatized forms. Signal enhancement of a 1 ions was proved to effectively aid in the full-length sequencing of small peptides. We further validated this method using two industrially-relevant protein-rich extracts from almond flour: 1737 medium-sized peptides (5-39 amino acids) and 843 small peptides (2-4 amino acids) were identified. The use of an online bioactive peptide database, complemented by the existing literature, allowed the discovery of 208 small bioactive peptides, whereas for medium-sized peptides, only one was reported being bioactive.
(Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)
فهرسة مساهمة: Keywords: Almonds (Prunus dulcis); Bioactive peptides; Di- and tripeptides; Dimethyl labeling; Enzymatic protein hydrolysis; Peptidomics
المشرفين على المادة: 0 (Peptides)
تواريخ الأحداث: Date Created: 20210905 Date Completed: 20211021 Latest Revision: 20211021
رمز التحديث: 20240628
DOI: 10.1016/j.foodchem.2021.130834
PMID: 34482238
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-7072
DOI:10.1016/j.foodchem.2021.130834