دورية أكاديمية
Antibacterial and bacteriostatic potential of coelomic fluid and body paste of Pheretima posthuma (Vaillant, 1868) (Clitellata, Megascolecidae) against ampicillin resistant clinical bacterial isolates.
| العنوان: | Antibacterial and bacteriostatic potential of coelomic fluid and body paste of Pheretima posthuma (Vaillant, 1868) (Clitellata, Megascolecidae) against ampicillin resistant clinical bacterial isolates. |
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| المؤلفون: | Hussain M; GC University, Department of Zoology, Microbiology Lab, Lahore, Pakistan., Liaqat I; GC University, Department of Zoology, Microbiology Lab, Lahore, Pakistan., Ali NM; GC University, Department of Zoology, Microbiology Lab, Lahore, Pakistan., Arshad N; University of Lahore, Institute of Molecular Biology and Biotechnology, Department of Zoology, Lahore, Pakistan., Hanif U; GC University, Department of Botany, Lahore, Pakistan., Sajjad S; Lahore College for Women University, Department of Zoology, Lahore, Pakistan., Sardar AA; GC University, Department of Botany, Lahore, Pakistan., Awan UF; GC University, Department of Botany, Lahore, Pakistan., Khan FS; University of Sialkot, Department of Biotechnology, Sialkot, Pakistan., Slahuddin; University of Gujrat, Department of Zoology, Gujrat, Pakistan. |
| المصدر: | Brazilian journal of biology = Revista brasleira de biologia [Braz J Biol] 2021 Sep 03; Vol. 83, pp. e247016. Date of Electronic Publication: 2021 Sep 03 (Print Publication: 2021). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: International Institute of Ecology Country of Publication: Brazil NLM ID: 101129542 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-4375 (Electronic) Linking ISSN: 15196984 NLM ISO Abbreviation: Braz J Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: São Carlos, SP [Brazil] : International Institute of Ecology, [2000]- |
| مواضيع طبية MeSH: | Oligochaeta* , Staphylococcus aureus*, Ampicillin/pharmacology ; Animals ; Anti-Bacterial Agents/pharmacology ; Microbial Sensitivity Tests |
| مستخلص: | Pheretima posthuma (Vaillant, 1868), a native earthworm of Pakistan and Southeast Asia, has wide utilization in vermicomposting and bioremediation process. In this study, P. posthuma coelomic fluid (PCF) and body paste (PBP) was evaluated as antibacterial agent against ampicillin (AMP) resistant five Gram positive and four Gram negative clinical isolates. The antibacterial effect of different doses (i.e. 25-100 µg/ml) of PCF and PBP along with AMP and azithromycin (AZM) (negative and positive controls, respectively) were observed through disc diffusion and micro-dilution methods. All nine clinical isolates were noticed as AMP resistant and AZM sensitive. Antibacterial effects of PCF and PBP were dose dependent and zone of inhibitions (ZI) against all clinical isolates were between 23.4 ± 0.92 to 0 ± 00 mm. The sensitivity profile of PCF and PBP against clinical isolates was noticed as 44.44 and 55.56%, respectively. Both PCF and PBP showed bacteriostatic (BTS) action against S. aureus, S. pyogenes, K. pneumonia, N. gonorrhoeae. Moreover, the cumulative BTS potential of PCF and PBP against all isolates was 66.67 and 55.56%, respectively. The MICs of PCF and PBP were ranged from 50-200 µg/ml against selected isolates. The bacterial growth curves indicated that PCF and PBP inhibited the growth of all isolates at their specific MIC concentrations. However, PBP has better antibacterial potential compared to PCF against selected isolates. Therefore, it is concluded that both PCF and PBP of P. posthuma possess antibacterial and BTS potential against ampicillin resistant clinical isolates. This organism might be considered as a second choice of antibacterial agents and can further be utilized in pharmaceutical industries for novel drug manufacturing by prospecting bioactive potential agents. |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 7C782967RD (Ampicillin) |
| تواريخ الأحداث: | Date Created: 20210908 Date Completed: 20210910 Latest Revision: 20210910 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1590/1519-6984.247016 |
| PMID: | 34495153 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1678-4375 |
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| DOI: | 10.1590/1519-6984.247016 |