دورية أكاديمية

Detection of microorganisms with antibacterial activities from different industrial wastes and GC-MS analysis of crude microbial extract.

التفاصيل البيبلوغرافية
العنوان: Detection of microorganisms with antibacterial activities from different industrial wastes and GC-MS analysis of crude microbial extract.
المؤلفون: Bano SA; The University of Haripur, Department of Microbiology, Khyber Pakhtunkhwa, Pakistan., Naz S; The University of Haripur, Department of Microbiology, Khyber Pakhtunkhwa, Pakistan., Uzair B; International Islamic University, Department of Biosciences, Islamabad, Pakistan., Hussain M; The University of Haripur, Department of Forestry and Wild Life, Khyber Pakhtunkhwa, Pakistan., Khan MM; The University of Haripur, Department of Microbiology, Khyber Pakhtunkhwa, Pakistan., Bibi H; Agricultural University, Department of Soil and Environmental Sciences, Peshawar, Pakistan., Habiba U; The University of Haripur, Department of Forestry and Wild Life, Khyber Pakhtunkhwa, Pakistan., Nisa S; The University of Haripur, Department of Microbiology, Khyber Pakhtunkhwa, Pakistan., Israr M; The University of Haripur, Department of Biology, Khyber Pakhtunkhwa, Pakistan.; Hebei Normal University Shijiazhuang, College of Life Science, Hebei, PR China.
المصدر: Brazilian journal of biology = Revista brasleira de biologia [Braz J Biol] 2021 Sep 06; Vol. 83, pp. e245585. Date of Electronic Publication: 2021 Sep 06 (Print Publication: 2021).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: International Institute of Ecology Country of Publication: Brazil NLM ID: 101129542 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-4375 (Electronic) Linking ISSN: 15196984 NLM ISO Abbreviation: Braz J Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: São Carlos, SP [Brazil] : International Institute of Ecology, [2000]-
مواضيع طبية MeSH: Industrial Waste* , Staphylococcus aureus*, Anti-Bacterial Agents/pharmacology ; Escherichia coli ; Gas Chromatography-Mass Spectrometry ; Humans ; Microbial Sensitivity Tests ; Plant Extracts ; RNA, Ribosomal, 16S
مستخلص: Many soil microorganisms' i.e., bacteria and fungi produce secondary metabolites called antibiotics. These are used for the treatment of some of the bacterial, fungal and protozoal diseases of humans. There is a need for isolation of a broad spectrum of antibiotics from microorganisms due to the emergence of antibiotic resistance. In the present study two antibiotic producing bacteria Klebsiella pneumoniae and Bacillus cereus were isolated from pharmaceutical and poultry feed industry of Hattar, Haripur Pakistan. Total 10 waste samples were collected from different industries (Marble, Ghee, Soap, Mineral, Steel, Poultry Feed, Pharmaceutical, Qarshi, Cosmetic and Glass). Thirty-three bacterial strains were isolated from industrial wastes of these ten different industries. Fourteen out of thirty-three bacterial strains exhibited antimicrobial activities against at least one of the test microbes considered in this study including Escherchia coli, Staphylococcus aureus and Salmonella typhi. The bacteria were isolated by standard serial dilution spread plate technique. Morphological characterization of the isolates was done by Gram staining. Nine bacterial isolates out of fourteen were initially identified as B. cereus and five as K. pneumoniae through biochemical characterization. The antibacterial activities were tested by well diffusion method. Maximum number of antibiotic producing bacteria were isolated from pharmaceutical and poultry feed industry based on the results of primary screening, the most potential isolates S9, S19, S20, S22 and S23 were selected for secondary screening. The maximum activity against E. coli and S. aureus was recorded by bacterial isolate S19 i.e zones of inhibition of 6.5mm and 9mm while S20 showed 7.5mm and 6mm zones respectively. Molecular identification was carried out on the basis of 16S rRNA sequence analysis. Finally, the isolates were identified as B. cereus accession number LC538271and K. pneumoniae accession number MT078679. Analysis of bacterial extract S20 through GC-MS indicated the presence of 8 compounds of diverse nature and structure. Present study suggests that wastes of pharmaceutical and poultry feed industry may have antibiotic producing bacteria. These bacteria could be utilized for the production of antibiotics. B. cereus and K. pneumoniae isolated from wastes of poultry feed and pharmaceutical industries have the potential to produce antibiotics and could be used to control the microbial growth.
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Industrial Waste)
0 (Plant Extracts)
0 (RNA, Ribosomal, 16S)
تواريخ الأحداث: Date Created: 20210908 Date Completed: 20210910 Latest Revision: 20210910
رمز التحديث: 20221213
DOI: 10.1590/1519-6984.245585
PMID: 34495157
قاعدة البيانات: MEDLINE
الوصف
تدمد:1678-4375
DOI:10.1590/1519-6984.245585