دورية أكاديمية
Exploring Effects of Protease Choice and Protease Combinations in Enzymatic Protein Hydrolysis of Poultry By-Products.
| العنوان: | Exploring Effects of Protease Choice and Protease Combinations in Enzymatic Protein Hydrolysis of Poultry By-Products. |
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| المؤلفون: | Lindberg D; Nofima AS, Osloveien 1, 1433 Ås, Norway., Kristoffersen KA; Nofima AS, Osloveien 1, 1433 Ås, Norway., Wubshet SG; Nofima AS, Osloveien 1, 1433 Ås, Norway., Hunnes LMG; Nofima AS, Osloveien 1, 1433 Ås, Norway., Dalsnes M; Nofima AS, Osloveien 1, 1433 Ås, Norway., Dankel KR; Nofima AS, Osloveien 1, 1433 Ås, Norway., Høst V; Nofima AS, Osloveien 1, 1433 Ås, Norway., Afseth NK; Nofima AS, Osloveien 1, 1433 Ås, Norway. |
| المصدر: | Molecules (Basel, Switzerland) [Molecules] 2021 Aug 31; Vol. 26 (17). Date of Electronic Publication: 2021 Aug 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, c1995- |
| مواضيع طبية MeSH: | Bromelains/*chemistry , Peptide Hydrolases/*metabolism, Animals ; Chickens ; Electrophoresis, Polyacrylamide Gel ; Temperature |
| مستخلص: | A study of the effects of single and combined protease hydrolysis on myofibrillar versus collagenous proteins of poultry by-products has been conducted. The aim was to contribute with knowledge for increased value creation of all constituents of these complex by-products. A rational approach was implemented for selecting proteases exhibiting the most different activity towards the major protein-rich constituents of mechanically deboned chicken residue (MDCR). An initial activity screening of 18 proteases on chicken meat, turkey tendons and MDCR was conducted. Based on weight yield, size exclusion chromatography (SEC) and SDS-PAGE, stem Bromelain and Endocut-02 were selected. Studies on hydrolysis of four different poultry by-products at 40 °C, evaluated by protein yield, SEC, and SDS-PAGE, indicate that the proteases' selectivity difference can be utilized in tailor-making hydrolysates, enriched in either meat- and collagen-derived peptides or gelatin. Three modes of stem Bromelain and Endocut-02 combinations during hydrolysis of MDCR were performed and compared with single protease hydrolysis. All modes of the protease combinations resulted in a similar approximately 15% increase in product yield, with products exhibiting similar SEC and SDS-PAGE profiles. This shows that irrespective of the modes of combination, the use of more than one enzyme in hydrolysis of collagen-rich material can provide means to increase the total protein yield and ultimately contribute to increased value creation of poultry by-products. |
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| معلومات مُعتمدة: | 280709 Norges Forskningsråd; 314599 Fondet for forskningsavgift på landbruksprodukter; 314111 Fondet for forskningsavgift på landbruksprodukter; 296083 Norges Forskningsråd |
| فهرسة مساهمة: | Keywords: Endocut-02; SDS-PAGE; SEC; enzymatic proteins hydrolysis; mechanically deboned chicken residue; poultry by-products; protease combinations; stem Bromelain |
| المشرفين على المادة: | 9001-00-7 (Bromelains) EC 3.4.- (Peptide Hydrolases) EC 3.4.22.32 (stem bromelain) |
| تواريخ الأحداث: | Date Created: 20210910 Date Completed: 20211213 Latest Revision: 20211214 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8434180 |
| DOI: | 10.3390/molecules26175280 |
| PMID: | 34500712 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1420-3049 |
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| DOI: | 10.3390/molecules26175280 |