دورية أكاديمية
Viability of Bacteriophages in the Complex Hydrogel Wound Dressings in vitro .
| العنوان: | Viability of Bacteriophages in the Complex Hydrogel Wound Dressings in vitro . |
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| المؤلفون: | Beschastnov VV; Associate Professor, Senior Researcher, University Clinic, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia., Ryabkov MG; Associate Professor, Leading Researcher, University Clinic, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia., Leontiev AE; Associate Professor, Senior Researcher, University Clinic, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia., Tulupov AA; Junior Researcher, University Clinic, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia., Yudanova TN; Head of the Laboratory, LLC 'New Dressing Materials', 2i Sergiev Posad District, Zhuchki, Moscow Region, 141351, Russia., Shirokova IY; Head of the Laboratory Research Department, University Clinic, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia., Belyanina NА; Junior Researcher, University Clinic, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia., Kovalishena OV; Professor, Head of the Department of Epidemiology, Microbiology and Evidence-Based Medicine, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia. |
| المصدر: | Sovremennye tekhnologii v meditsine [Sovrem Tekhnologii Med] 2021; Vol. 13 (2), pp. 32-38. Date of Electronic Publication: 2021 Apr 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nizhegorodskai︠a︡ gosudarstvennai︠a︡ medit︠s︡inskai︠a︡ akademii︠a Country of Publication: Russia (Federation) NLM ID: 101604515 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2309-995X (Electronic) Linking ISSN: 20764243 NLM ISO Abbreviation: Sovrem Tekhnologii Med |
| أسماء مطبوعة: | Original Publication: Nizhniĭ Novgorod : Nizhegorodskai︠a︡ gosudarstvennai︠a︡ medit︠s︡inskai︠a︡ akademii︠a︡ |
| مواضيع طبية MeSH: | Bacteriophages*, Bandages ; Hydrogels ; Polyvinyl Alcohol ; Staphylococcus aureus |
| مستخلص: | Using bacteriophages to overcome the increasing resistance of microorganisms to antibiotics is a novel research venue of clinical importance. Among other challenges, this technique is expected to create and maintain an adequate local concentration of bacteriophages at the site of application. In addition, the possibility of combining the phage preparation with antioxidants and anesthetics may provide new options for stimulating the reparative process. The aim of the study was to assess the viability and lytic activity of bacteriophages incorporated into a hydrogel-based wound dressing that contains polyvinyl alcohol, phosphate buffer, with optional additions of succinic acid and lidocaine. Materials and Methods: A technique for incorporating bacteriophages into the complex hydrogel wound dressing ex tempore has been proposed. The bacteriolytic activity of phages inside the hydrogel was determined using standard microbiological techniques. Specifically, we used nutrient media with lawn cultures of Staphylococcus aureus added with the following antibacterial combinations: bacteriophages + succinic acid, bacteriophages + lidocaine, and bacteriophages + succinic acid + lidocaine. The lytic activity of bacteriophages was assessed within 1 to 7 days after the formation of the hydrogel. Results: In all samples containing bacteriophages, the presence of viable and lytically active phages was noted within 1 to 7 days, as evidenced by the "negative colonies" on the culture lawns. On days 1 to 3, no secondary growth was recorded in the phage-containing samples. In hydrogel samples containing phages, succinic acid, and lidocaine, secondary bacterial colonies were detected starting from day 4 indicating some reduction in the lytic activity. Conclusion: The results suggest that bacteriophages immobilized in the hydrogel maintain their viability and lytic activity, and this activity persists when the phages are combined with succinic acid and lidocaine. Competing Interests: Conflict of interest. There is no conflict of interest among the authors and other interested parties. |
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| فهرسة مساهمة: | Keywords: bacteriophages; hydrogel; lidocaine; succinic acid; wound dressing |
| المشرفين على المادة: | 0 (Hydrogels) 9002-89-5 (Polyvinyl Alcohol) |
| تواريخ الأحداث: | Date Created: 20210913 Date Completed: 20211210 Latest Revision: 20211214 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8353722 |
| DOI: | 10.17691/stm2021.13.2.03 |
| PMID: | 34513074 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2309-995X |
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| DOI: | 10.17691/stm2021.13.2.03 |