دورية أكاديمية
أعرض في EDS

Brain-derived neurotrophic factor predominantly regulates the expression of synapse-related genes in the striatum: Insights from in vitro transcriptomics.

التفاصيل البيبلوغرافية
العنوان: Brain-derived neurotrophic factor predominantly regulates the expression of synapse-related genes in the striatum: Insights from in vitro transcriptomics.
المؤلفون: Koshimizu H; Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Japan., Matsuoka H; Department of Pharmaceutical Science, Yokohama University of Pharmacy, Yokohama, Japan.; School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan., Nakajima Y; Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Japan., Kawai A; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan., Ono J; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan., Ohta KI; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan., Miki T; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan., Sunagawa M; Department of Physiology, Showa University School of Medicine, Tokyo, Japan., Adachi N; Department of Physiology, Showa University School of Medicine, Tokyo, Japan.; Department of Mental Disorder Research, National Center of Neurology and Psychiatry (NCNP), Tokyo, Japan., Suzuki S; Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Japan.; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
المصدر: Neuropsychopharmacology reports [Neuropsychopharmacol Rep] 2021 Dec; Vol. 41 (4), pp. 485-495. Date of Electronic Publication: 2021 Sep 16.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: John Wiley & Sons, Inc Country of Publication: United States NLM ID: 101719700 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2574-173X (Electronic) Linking ISSN: 2574173X NLM ISO Abbreviation: Neuropsychopharmacol Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Hoboken, NJ] : John Wiley & Sons, Inc., [2018]-
مواضيع طبية MeSH: Brain-Derived Neurotrophic Factor*/genetics , Corpus Striatum*/metabolism , Transcriptome*, Animals ; Neurons/metabolism ; Rats ; Synapses/metabolism
مستخلص: Aim: The striatum, a main component of the basal ganglia, is a critical part of the motor and reward systems of the brain. It consists of GABAergic and cholinergic neurons and receives projections of dopaminergic, glutamatergic, and serotonergic neurons from other brain regions. Brain-derived neurotrophic factor (BDNF) plays multiple roles in the central nervous system, and striatal BDNF has been suggested to be involved in psychiatric and neurodegenerative disorders. However, the transcriptomic impact of BDNF on the striatum remains largely unknown. In the present study, we performed transcriptomic profiling of striatal cells stimulated with BDNF to identify enriched gene sets (GSs) and their novel target genes in vitro.
Methods: We carried out RNA sequencing (RNA-Seq) of messenger RNA extracted from primary dissociated cultures of rat striatum stimulated with BDNF and conducted Generally Applicable Gene-set Enrichment (GAGE) analysis on 10599 genes. Significant differentially expressed genes (DEGs) were determined by differential expression analysis for sequence count data 2 (DESeq2).
Results: GAGE analysis identified significantly enriched GSs that included GSs related to regulation and dysregulation of synaptic functions, such as synaptic vesicle cycle and addiction to nicotine and morphine, respectively. It also detected GSs related to various types of synapses, including not only GABAergic and cholinergic synapses but also dopaminergic and glutamatergic synapses. DESeq2 revealed 72 significant DEGs, among which the highest significance was observed in the apolipoprotein L domain containing 1 (Apold1).
Conclusions: The present study indicates that BDNF predominantly regulates the expression of synaptic-function-related genes and that BDNF promotes synaptogenesis in various subtypes of neurons in the developing striatum. Apold1 may represent a unique target gene of BDNF in the striatum.
(© 2021 The Authors. Neuropsychopharmacology Reports published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Neuropsychopharmacology.)
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فهرسة مساهمة: Keywords: Apold1; BDNF; DESeq2; GAGE; RNA-Seq; striatum
المشرفين على المادة: 0 (Bdnf protein, rat)
0 (Brain-Derived Neurotrophic Factor)
تواريخ الأحداث: Date Created: 20210916 Date Completed: 20220323 Latest Revision: 20220531
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC8698681
DOI: 10.1002/npr2.12208
PMID: 34529365
قاعدة البيانات: MEDLINE
الوصف
تدمد:2574-173X
DOI:10.1002/npr2.12208