دورية أكاديمية
Satb2 Regulates EphA7 to Control Soma Spacing and Self-Avoidance of Cortical Pyramidal Neurons.
| العنوان: | Satb2 Regulates EphA7 to Control Soma Spacing and Self-Avoidance of Cortical Pyramidal Neurons. |
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| المؤلفون: | He CH; Key Laboratory of Arrhythmias, Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai 200120, China.; Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai 200092, China., Zhang L; Key Laboratory of Arrhythmias, Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai 200120, China.; Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai 200092, China., Song NN; Department of Laboratory Animal Science, Fudan University, Shanghai 200032, China.; State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai 200032, China., Mei WY; Key Laboratory of Arrhythmias, Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai 200120, China.; Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai 200092, China., Chen JY; Department of Laboratory Animal Science, Fudan University, Shanghai 200032, China.; State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai 200032, China., Hu L; State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai 200032, China., Zhang Q; Department of Laboratory Animal Science, Fudan University, Shanghai 200032, China.; State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai 200032, China., Wang YB; Key Laboratory of Arrhythmias, Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai 200120, China.; Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai 200092, China., Ding YQ; Key Laboratory of Arrhythmias, Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai 200120, China.; Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai 200092, China.; Department of Laboratory Animal Science, Fudan University, Shanghai 200032, China.; State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai 200032, China. |
| المصدر: | Cerebral cortex (New York, N.Y. : 1991) [Cereb Cortex] 2022 May 30; Vol. 32 (11), pp. 2321-2331. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 9110718 Publication Model: Print Cited Medium: Internet ISSN: 1460-2199 (Electronic) Linking ISSN: 10473211 NLM ISO Abbreviation: Cereb Cortex Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Oxford University Press, c1991- |
| مواضيع طبية MeSH: | Cerebral Cortex*/metabolism , Neurons*/metabolism, Animals ; Cell Body/metabolism ; Matrix Attachment Region Binding Proteins ; Mice ; Pyramidal Cells/metabolism ; Receptor, EphA7 ; Transcription Factors/metabolism |
| مستخلص: | Soma spacing and dendritic arborization during brain development are key events for the establishment of proper neural circuitry and function. Transcription factor Satb2 is a molecular node in regulating the development of the cerebral cortex, as shown by the facts that Satb2 is required for the regionalization of retrosplenial cortex, the determination of callosal neuron fate, and the regulation of soma spacing and dendritic self-avoidance of cortical pyramidal neurons. In this study, we explored downstream effectors that mediate the Satb2-implicated soma spacing and dendritic self-avoidance. First, RNA-seq analysis of the cortex revealed differentially expressed genes between control and Satb2 CKO mice. Among them, EphA7 transcription was dramatically increased in layers II/III of Satb2 CKO cortex. Overexpression of EphA7 in the late-born cortical neurons of wild-type mice via in utero electroporation resulted in soma clumping and impaired self-avoidance of affected pyramidal neurons, which resembles the phenotypes caused by knockdown of Satb2 expression. Importantly, the phenotypes by Satb2 knockdown was rescued by reducing EphA7 expression in the cortex. Finally, ChIP and luciferase reporter assays indicated a direct suppression of EphA7 expression by Satb2. These findings provide new insights into the complexity of transcriptional regulation of the morphogenesis of cerebral cortex. (© The Author(s) 2021. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.) |
| فهرسة مساهمة: | Keywords: EphA7; Satb2; in utero electroporation; self-avoidance; soma spacing |
| المشرفين على المادة: | 0 (Matrix Attachment Region Binding Proteins) 0 (SATB2 protein, mouse) 0 (Transcription Factors) EC 2.7.10.1 (Receptor, EphA7) |
| تواريخ الأحداث: | Date Created: 20210921 Date Completed: 20220602 Latest Revision: 20220723 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1093/cercor/bhab321 |
| PMID: | 34546353 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1460-2199 |
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| DOI: | 10.1093/cercor/bhab321 |