دورية أكاديمية
CENP-B promotes the centromeric localization of ZFAT to control transcription of noncoding RNA.
| العنوان: | CENP-B promotes the centromeric localization of ZFAT to control transcription of noncoding RNA. |
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| المؤلفون: | Ishikura S; Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan; Center for Advanced Molecular Medicine, Fukuoka University, Fukuoka, Japan., Yoshida K; Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan; Center for Advanced Molecular Medicine, Fukuoka University, Fukuoka, Japan., Hashimoto S; Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan., Nakabayashi K; Department of Maternal-Fetal Biology, National Research Institute for Child Health and Development, Tokyo, Japan., Tsunoda T; Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan; Center for Advanced Molecular Medicine, Fukuoka University, Fukuoka, Japan., Shirasawa S; Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan; Center for Advanced Molecular Medicine, Fukuoka University, Fukuoka, Japan. Electronic address: sshirasa@fukuoka-u.ac.jp. |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2021 Oct; Vol. 297 (4), pp. 101213. Date of Electronic Publication: 2021 Sep 20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Transcription, Genetic*, Centromere/*metabolism , Centromere Protein B/*metabolism , RNA, Untranslated/*biosynthesis , Transcription Factors/*metabolism, Animals ; Centromere/genetics ; Centromere Protein B/genetics ; HEK293 Cells ; HeLa Cells ; Humans ; Mice ; NIH 3T3 Cells ; RNA, Untranslated/genetics ; Transcription Factors/genetics |
| مستخلص: | The centromere is a chromosomal locus that is essential for the accurate segregation of chromosomes during cell division. Transcription of noncoding RNA (ncRNA) at the centromere plays a crucial role in centromere function. The zinc-finger transcriptional regulator ZFAT binds to a specific 8-bp DNA sequence at the centromere, named the ZFAT box, to control ncRNA transcription. However, the precise molecular mechanisms by which ZFAT localizes to the centromere remain elusive. Here we show that the centromeric protein CENP-B is required for the centromeric localization of ZFAT to regulate ncRNA transcription. The ectopic expression of CENP-B induces the accumulation of both endogenous and ectopically expressed ZFAT protein at the centromere in human cells, suggesting that the centromeric localization of ZFAT requires the presence of CENP-B. Coimmunoprecipitation analysis reveals that ZFAT interacts with the acidic domain of CENP-B, and depletion of endogenous CENP-B reduces the centromeric levels of ZFAT protein, further supporting that CENP-B is required for the centromeric localization of ZFAT. In addition, knockdown of CENP-B significantly decreased the expression levels of ncRNA at the centromere where ZFAT regulates the transcription, suggesting that CENP-B is involved in the ZFAT-regulated centromeric ncRNA transcription. Thus, we concluded that CENP-B contributes to the establishment of the centromeric localization of ZFAT to regulate ncRNA transcription. Competing Interests: Conflict of interest The authors declare that there are no conflicts of interest with the contents of this article. (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: CENP-B; DNA binding protein; ZFAT; centromere; noncoding RNA; protein-protein interaction; transcription regulation; zinc finger |
| المشرفين على المادة: | 0 (CENPB protein, human) 0 (Centromere Protein B) 0 (RNA, Untranslated) 0 (Transcription Factors) 0 (ZFAT protein, human) 0 (ZFAT protein, mouse) |
| تواريخ الأحداث: | Date Created: 20210921 Date Completed: 20211123 Latest Revision: 20211123 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8496178 |
| DOI: | 10.1016/j.jbc.2021.101213 |
| PMID: | 34547289 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1083-351X |
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| DOI: | 10.1016/j.jbc.2021.101213 |