Inhibitory effect of Pyr6 (an Orai channel blocker) on agonist-induced contractions in rat uterus.

التفاصيل البيبلوغرافية
العنوان:	Inhibitory effect of Pyr6 (an Orai channel blocker) on agonist-induced contractions in rat uterus.
المؤلفون:	de Sousa ÍA; Biophysics and Physiology Department, Health Sciences Center, Federal University of Piauí, Ininga, Teresina, Brazil., de Meneses GMS; Biophysics and Physiology Department, Health Sciences Center, Federal University of Piauí, Ininga, Teresina, Brazil., Cardoso JVM; Biophysics and Physiology Department, Health Sciences Center, Federal University of Piauí, Ininga, Teresina, Brazil., Lopes PQ; Pharmacological Sciences Department, Health Sciences Center, Federal University of Paraíba, Cidade Universitária - Campus I. Castelo Branco, João Pessoa, Brazil., de Sousa JA; Physiotherapy Department, Health Sciences Center, University of the State of Piauí, Teresina, Brazil., Cavalcanti SMPG; Physiotherapy Department, Health Sciences Center, University of the State of Piauí, Teresina, Brazil., da Silva Cavalcanti PM; Pharmacological Sciences Department, Health Sciences Center, Federal University of Paraíba, Cidade Universitária - Campus I. Castelo Branco, João Pessoa, Brazil., Filho FC; Biophysics and Physiology Department, Health Sciences Center, Federal University of Piauí, Ininga, Teresina, Brazil.
المصدر:	The journal of obstetrics and gynaecology research [J Obstet Gynaecol Res] 2021 Dec; Vol. 47 (12), pp. 4306-4318. Date of Electronic Publication: 2021 Sep 27.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Wiley on behalf of the Japan Society of Obstetrics and Gynecology] Country of Publication: Australia NLM ID: 9612761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1447-0756 (Electronic) Linking ISSN: 13418076 NLM ISO Abbreviation: J Obstet Gynaecol Res Subsets: MEDLINE
أسماء مطبوعة:	Publication: [Melbourne, Vic. : Wiley on behalf of the Japan Society of Obstetrics and Gynecology] Original Publication: Tokyo : University of Tokyo Press, c1996-
مواضيع طبية MeSH:	Myometrium* , Uterine Contraction*, Animals ; Female ; Oxytocin ; Pregnancy ; Rats
مستخلص:	Aim: Both human and rat myometrium express stromal interaction molecule (STIM) and Orai/transient receptor potential canonical (TRPC) proteins, which are components of plasma membrane Ca ²⁺ store-operated channels. There are reports that these proteins mediate agonist-induced Ca ²⁺ influx in cultured myometrial cells. In this study, we aimed to determine the effects of Pyr6, an Orai channel blocker, on different agonist-induced contractions in isolated segments of rat uterus. Main Findings: In Ca ²⁺ -free Tyrode's solution, Pyr6 (3 μM) promoted a reduction in both the magnitude and frequency of Ca ²⁺ (1 mM)-induced uterine contractions after the addition of carbachol (CCh, 100 μM), but not after the addition of oxytocin (OT, 150 nM). In Ca ²⁺ (0.18 mM)-Tyrode's solution, Pyr6 completely relaxed uterine contractions induced by both CCh and cloprostenol (300 nM), but not those induced by either KCI (40-80 mM) or OT. The addition of Pyr6 abolished the oscillatory uterine contractions induced by Ca ²⁺ after the addition of cyclopiazonic acid (CPA, 10 μM). When pre-incubated (5 min), Pyr6 reduced the magnitude of both CCh-induced phasic and tonic contractions. The addition of Pyr2 (3 μM), an Orai and TRPC channel blocker, abolished uterine contractions induced by CCh or OT. Conclusion: Considering Pyr6 as an Orai channel blocker and its inhibitory effect on uterine contractions induced by CCh, CPA, and cloprostenol, we suggest that Orai channels are required for the maintenance of contractions induced by these agonists in rat uterus. (© 2021 Japan Society of Obstetrics and Gynecology.)
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فهرسة مساهمة:	Keywords: Orai channels; Pyr6; TRPC channels; rat uterus; uterine contractions
المشرفين على المادة:	50-56-6 (Oxytocin)
تواريخ الأحداث:	Date Created: 20210927 Date Completed: 20211203 Latest Revision: 20211214
رمز التحديث:	20240628
DOI:	10.1111/jog.15034
PMID:	34571573
قاعدة البيانات:	MEDLINE

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تدمد:	1447-0756
DOI:	10.1111/jog.15034