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Is It Possible to Create Antimicrobial Peptides Based on the Amyloidogenic Sequence of Ribosomal S1 Protein of P. aeruginosa ?

التفاصيل البيبلوغرافية
العنوان: Is It Possible to Create Antimicrobial Peptides Based on the Amyloidogenic Sequence of Ribosomal S1 Protein of P. aeruginosa ?
المؤلفون: Grishin SY; Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Russia., Domnin PA; Gamaleya Research Centre of Epidemiology and Microbiology, 123098 Moscow, Russia.; Biology Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia., Kravchenko SV; Institute of Environmental and Agricultural Biology (X-BIO), Tyumen State University, 625003 Tyumen, Russia., Azev VN; The Branch of the Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Russia., Mustaeva LG; The Branch of the Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Russia., Gorbunova EY; The Branch of the Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Russia., Kobyakova MI; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia., Surin AK; Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Russia.; The Branch of the Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Russia.; State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia., Makarova MA; State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia., Kurpe SR; Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Russia., Fadeev RS; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia., Vasilchenko AS; Institute of Environmental and Agricultural Biology (X-BIO), Tyumen State University, 625003 Tyumen, Russia., Firstova VV; State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia., Ermolaeva SA; Gamaleya Research Centre of Epidemiology and Microbiology, 123098 Moscow, Russia., Galzitskaya OV; Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Russia.; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2021 Sep 10; Vol. 22 (18). Date of Electronic Publication: 2021 Sep 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Amyloidogenic Proteins/*genetics , Pore Forming Cytotoxic Proteins/*genetics , Pseudomonas aeruginosa/*drug effects , Ribosomal Proteins/*genetics, Amyloidogenic Proteins/chemistry ; Amyloidogenic Proteins/pharmacology ; Amyloidogenic Proteins/ultrastructure ; Anti-Bacterial Agents/adverse effects ; Humans ; Microbial Sensitivity Tests ; Pore Forming Cytotoxic Proteins/chemistry ; Pore Forming Cytotoxic Proteins/pharmacology ; Protein Structure, Secondary ; Pseudomonas aeruginosa/pathogenicity ; Ribosomal Proteins/pharmacology ; Ribosomal Proteins/ultrastructure
مستخلص: The development and testing of new antimicrobial peptides (AMPs) represent an important milestone toward the development of new antimicrobial drugs that can inhibit the growth of pathogens and multidrug-resistant microorganisms such as Pseudomonas aeruginosa, Gram-negative bacteria. Most AMPs achieve these goals through mechanisms that disrupt the normal permeability of the cell membrane, which ultimately leads to the death of the pathogenic cell. Here, we developed a unique combination of a membrane penetrating peptide and peptides prone to amyloidogenesis to create hybrid peptide: "cell penetrating peptide + linker + amyloidogenic peptide". We evaluated the antimicrobial effects of two peptides that were developed from sequences with different propensities for amyloid formation. Among the two hybrid peptides, one was found with antibacterial activity comparable to antibiotic gentamicin sulfate. Our peptides showed no toxicity to eukaryotic cells. In addition, we evaluated the effect on the antimicrobial properties of amino acid substitutions in the non-amyloidogenic region of peptides. We compared the results with data on the predicted secondary structure, hydrophobicity, and antimicrobial properties of the original and modified peptides. In conclusion, our study demonstrates the promise of hybrid peptides based on amyloidogenic regions of the ribosomal S1 protein for the development of new antimicrobial drugs against P. aeruginosa .
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معلومات مُعتمدة: 18-14-00321 Russian Science Foundation
فهرسة مساهمة: Keywords: Pseudomonas aeruginosa; amyloid; antimicrobial peptides; cell penetrating peptide; ribosomal S1 protein
المشرفين على المادة: 0 (Amyloidogenic Proteins)
0 (Anti-Bacterial Agents)
0 (Pore Forming Cytotoxic Proteins)
0 (Ribosomal Proteins)
0 (ribosomal protein S1)
تواريخ الأحداث: Date Created: 20210928 Date Completed: 20211022 Latest Revision: 20211022
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8469417
DOI: 10.3390/ijms22189776
PMID: 34575940
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms22189776